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How to prepare hormonal stock solution//Plant tissue culture//Phytohormones//Plant growth regulators

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#Auxins #Cytokinins #Gibberellins
Watch this video completely to know what are hormones, how do we use them in what concentrations and how to prepare their stock solutions.
Click to watch:
-- Filter sterilization of hormones: • Filter sterilization//...
--MS Media preparation: • How to prepare MS medi...
--Part-1 of Hairy Root culture: • Hairy root culture//Bi...
--Part-2 of Hairy root culture: • Hairy root culture - P...
--Cell suspension culture: • Cell Suspension Cultur...
--Embryo culture: • Plant EMBRYO Culture//...
--Callus induction: • Callus induction proce...
--Callus induction from seed: • Callus induction from ...
--The stages of in-vitro seed germination: • Stages of In-vitro see...
--To learn the process of in-vitro seed germination watch this video: • Invitro seed germinati...
-- To learn basics of sterilization techniques watch this video: • Types of Sterilization...
-- To learn all about the Tissue culture lab, the instruments used watch this video: • Plant Tissue culture L...
-- Learn how to fumigate the lab by watching this video : • Fumigation of Lab
-- Visual tour of microbiology lab: • Microbiology lab and t...
-- Visual tour of molecular biology lab: • Molecular biology lab
-- Visual tour of a general life science laboratory: • Life science research ...

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5 июл 2021

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Комментарии : 76

@jabakarphilipson9571 2 года назад

Dont lose hope by low amount of viewers, you are doing a great job, we need you, king 👑 .

@Dr.Rakesh.B 2 года назад

Thank you. Much needed words :)

@iTz_ROLEX 4 месяца назад

Thank you so much sir.. Your videos are amazing...!! 🙏❤️

@beepboopbs98 9 месяцев назад

Thankyou for sharing your lab tips and wisdom.

@takshanayakpraveena3771 Год назад

Thank you so much very very useful... We would expect more like this basic level of tissue culture work s... Thank you so much bro...🎉🎉🎉🎉

@aikrathod165 9 месяцев назад

Clarity level 💯 sir, thank you

@analesaskeete9343 2 года назад

Thank you for your very informative videos.

@zenlasmo 8 месяцев назад

Thank you sir please keep on making videos we need your guidance and teaching

@arpitamandalkhan6080 2 года назад

Very nice video... very informative and detailed.. thanks a lot

@Dr.Rakesh.B 2 года назад

Thank you:)

@amtd3808 3 года назад

Thank you sir..👍...Very informative video..👌👌

@Dr.Rakesh.B 3 года назад

Your welcome. I have made it specifically for you as you had requested many times

@anyadevine4094 Год назад

Thank you ❤

@sachinlimaye9103 2 года назад

Thanks sir God bless you

@Dr.Rakesh.B 2 года назад

🙏

@shivramfarmnursery2325 4 месяца назад

i seen your all videos i like it i want to made papaya tissue culter for trail basics can you help me

@namarajasekhar 3 года назад

Thank you very much sir

@namarajasekhar 3 года назад

could you drop your mail pls

@prasodhanniraula3356 Месяц назад

How can we download the PGR reference from either sigma or photo tech? i dint found

@yashikanalla299 6 месяцев назад

Sir can you make a video on how to make stock solutions of Mineral salt Media how to prepare it.

@RusticRaver Год назад

Hi man, great video! One question about PPM plant preservative mixture, do you use it?; I see some companies selling this to avoid contamination, very expensive, can we make some ourselves using fungicid and antibacterial solution? I did find Miconazole nitrate in Daktarin powder(medical talk fungal infection) any ideas?

@Dr.Rakesh.B Год назад

No I don't use PPM. Without adding any antimicrobial agents it is possible to get contamination free cultures with practice and maintaining proper sterile ambience. If you want you can add, but it might affect the culture.

@janvi2283 5 месяцев назад

How can we store stock solution of bavistin?

@navnathlokhande-v9t 23 часа назад

very nice sir ,sir can you make this vidio in matathi or hindi langvage for farmer

@mohmmedbahi 2 года назад

Thanks alot

@mohmmedbahi 2 года назад

If I have information about growth hormones send me

@Dr.Rakesh.B 2 года назад

It's available in internet. I don't have any currently.

@iiiiiieye 8 месяцев назад

Whats the name of that dropper plastic bottle???? cant find it online anywhere

@Dr.Rakesh.B 8 месяцев назад

We call it Reagent bottle or dropper bottle. Try from Vasa Scientific and co

@arohishermale515 Год назад

Sir MG Te selective medium composition milel ka

@subalbairagi1379 Месяц назад

Sir Iss hormone ko kebal or kebal ethanol may dilute korkey 1 sal Tak room temperature may store kor saktey hai Distal water mix Nehi korkey

@savitasandur2037 2 года назад

Hi sir, very informative vedios. Sir I need one clarification, for the purification of Auxin which is synthesized by Actinomycetes, need to do GC-MS. So how should I sent the cultures for the GC-MS.? Plz clarify sir.

@Dr.Rakesh.B 2 года назад

Hi, it depends on the type of instrument. So better to ask the person who is incharge of that instrument.

@savitasandur2037 2 года назад

@@Dr.Rakesh.B k sir thank you

@plantscienceswithprasanthi1834 Год назад

Nice explanation bro, I am doing PTC research work with medicinal orchids, my doubt is when we have to add plant hormones stock to media? Before checking pH or after checking pH ....?

@Dr.Rakesh.B Год назад

Before checking pH

@subalbairagi1379 8 месяцев назад

Sir Poiley to upko bahut bahut dhanyawad Q ki up savi comments ka jabab dete ho Sir Mera pass 4 Chloro phenoxy acitic acid (4-cpa) powder hay A ak auxin hormone Mai is powder ko liquid form ma banakor 1 sal tak room temperature may rakna chata huu To iske liye muzey kya korna porega

@Dr.Rakesh.B 8 месяцев назад

You cannot keep it until a year Store as powder form only in deep freezer make stock and use it within 2-3 months

@nutube6291 3 года назад

KOH is better as NaOH (dissociated sodium ion) is not favorable for plant growth. What are your thoughts on this?

@Dr.Rakesh.B 3 года назад

A few drops of NaOH is added to dissolve so that wont affect the plants. And sodium is a macro element required for plant growth and development. So naoh is better than koh bcoz high concentration of potassium is more damaging than sodium

@nutube6291 3 года назад

@@Dr.Rakesh.B Thanks for getting back.

@ahlamabdulaziz6513 Год назад

can we do similar preparation method to 2,4-D?..

@Dr.Rakesh.B Год назад

Yes

@rushikeshsalunkhe2179 3 года назад

Hello sir Is there need govt. Permission to study & experiment tissue culture at home or setup a small lab.

@Dr.Rakesh.B 3 года назад

No not required you can set up a home lab also to experiment and study

@amtd3808 3 года назад

The only problem is Laminar Air Flow unit coz it is very costly..

@daniinolte5486 2 года назад

Good day sir! I have a question: 1. can you sterilize PGR Stock solution in microwave instead of in autoclave? 2. How does synringe sterilization of PGR stock solution work?do you have a video of that? Kind regards

@Dr.Rakesh.B 2 года назад

Yes I have made a separate video on filter sterilization which is there in my channel do watch it. In microwave the temp and pressure is not controlled.

@daniinolte5486 2 года назад

@@Dr.Rakesh.B Thank you for your speedy assistance! Kind regards

@asitbasu7657 Год назад

how to use growth retardent in bonsai? its doses also

@prajwalaprakash6590 3 года назад

Sir please make video on anther culture

@Dr.Rakesh.B 3 года назад

Sure:)

@subalbairagi1379 6 месяцев назад

CA = coautoclavable with other media components. F = filter sterlize. CA/F = coautoclavable with other media components, however, some loss of activity may occur. This can be compensated for by increasing component concentration. Component may be filter sterilized. Sir CA = ka matalab kya hay

@danieljheelan5256 3 года назад

Hi, I am interested in TC of orchids and this link is very useful. Never prepared any stock solution and have bought ANA and BAP to clone orchids stems. I will accept all help. Can you share the link to download the pdf file from Sigma Aldrich. Thanks Shiam Daniel Jheelan

@Dr.Rakesh.B 3 года назад

www.sigmaaldrich.com/IN/en/technical-documents/technical-article/cell-culture-and-cell-culture-analysis/plant-tissue-culture/growth-regulators There are many more from different websites. You can browse and check out to select which ever is comfortable for you.

@danieljheelan5256 3 года назад

@@Dr.Rakesh.B Hi, Thanks a lot for your reply. Shiam

@deepbhushan2050 Год назад

Can we mix cutokinin and auxin

@Dr.Rakesh.B Год назад

Yes

@deepakjawale2941 2 года назад

sir how to germinate banana plant with hormone

@Dr.Rakesh.B 2 года назад

Download papers from Google Scholar

@kalpanarautela320 2 года назад

Sir, do we need to add vitamins and hormones(BAP, NAA, 2 4-D, Tdz) to the media before autoclaving or after autoclave ?

@Dr.Rakesh.B 2 года назад

Yes you can add them before. I always add before only

@Pk12344 3 года назад

How to know that hormonal conformation?

@Dr.Rakesh.B 3 года назад

What do you mean by hormonal confirmation?

@Pk12344 3 года назад

Conformation means whether the hormone is in cis or trans position.

@cl7max 9 месяцев назад

Here In Germany NaOh is called Natronlauge. But now Im calling it Änaewitch myself 😂😂

@cl7max 9 месяцев назад

Also really good Video learned a Lot of things

@bonekliarpadangan1527 Год назад

How many % Naoh DR ?

@Dr.Rakesh.B Год назад

Prepare 0.5M or 1.0 M solution. And use

@parasbohara2276 2 года назад

Dear sir, I have been watching your video and found it very informative. If possible can you please provide me your email address so that I can ask for more detailed information about plant tissue culture. Thank you in advance.

@Dr.Rakesh.B 2 года назад

rakeshb96@yahoo.com

@nishanth6373 2 года назад

Hiii bro....Please send me a required media composition completely and I will derive the steps how to get required concentration and send you. Then you check it whether I have calculated correctly.

@Dr.Rakesh.B 2 года назад

The composition is available in the net from many sources. Also in video i have shown. Pls check