How to study cells - Microscopes, magnification and calibrating the eyepiece graticule

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Learn the methods to study cells for AQA A-level biology. Learn the differences between optical and electron microscopes, how to calculate magnification and how to calibrate the eyepiece graticule.
This is a long video, so below are the time stamps for each section. It is one of the top requested topics in revision lessons, so if you have the time I highly recommend watching the entire video
Microscopes: 1.17mins
Magnification: 7.15mins
Eyepiece Graticule: 11.05mins
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11 июл 2024

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Комментарии : 64

@resurrectionstone9217 4 года назад

Thank you so much! I literally went through tonnes of videos but didn't understand the concept of the eyepiece graticule. You made it so much easier!

@MissEstruchBiology 4 года назад

Thank you, really glad to hear it helped you :)

@mohid0786 2 года назад

She acc did as well tho 😭 Legit helped me out so much, y did I not discover u in September or in the summer

@emmie_vukic4169 Год назад

I have my biology mock tomorrow and I've been watching your videos non stop so i really hope they've helped :)))

@MissEstruchBiology Год назад

Good luck!!!!

@doormat-wr9su Год назад

Good luck!

@lifeofluke7136 3 года назад

Just want to say thank you so much for your videos, they're so helpful, and good for revision for my exam tmrw!

@MissEstruchBiology 3 года назад

Thank you!!! So pleased they are helping you. Good luck for your exam tomorrow 🤞🤞

@s_mehmi28 2 года назад

big up miss Estruch for helping me out

@MissEstruchBiology 2 года назад

haha thanks! Glad the videos helped

@nayaa964 Год назад

Hi you are my new biology teacher 🙃

@MissEstruchBiology Год назад

😄😄 hello 👋🏻 welcome to the class

@sampadapoudel1989 3 года назад

thankyou, really helped me.

@MissEstruchBiology 3 года назад

Glad it helped!

@tahakarim1901 Год назад

Thank you so much for the help! Can I please ask, do you have all the information we need for AQA A-Level Biology covered in your videos or should we use the textbooks alongside these videos? Thanks again!

@MissEstruchBiology Год назад

they cover all the theory for AQA A level. 😊

@winlarter4235 4 года назад

Hi Miss Thank you for your video ! much appreciated I wanted to ask what does calibrate means?

@MissEstruchBiology 4 года назад

Calibrating equipment is when you adjust or mark the equipment so that it can be used to measure in an accurate way. In this instance, with microscopes, when we calibrate it means we are working out exact what each division distance represents at each magnification in the eye piece graticule (the ruler inside the eyepiece). This enables us to then accurate measure the structures we are viewing under the microscope.

@tommocymraeg 3 года назад

Diolch yn fawr! Really helpful for studies.

@MissEstruchBiology 3 года назад

thank you :) Really pleased it helped you.

@moa7651 Год назад

miss so does 1 division on a stage micrometre always = 10MicroMetre

@unimoon3862 2 года назад

Hi, one thing I don’t understand is how does a division on the eyepiece graticule equal 5micrometers and not 20micrometers? I’ve been thinking that as 2 divisions of the eyepiece go into 1 division of the stage micrometer, then we need to times by 10 to get the eyepiece division micrometer? 😅😅 I can’t seem to see where I’ve gone wrong

@akshitamodi8222 Год назад

I'm pretty sure she meant 1mm instead of 2mm

@user-vq4qy2hq5i 10 месяцев назад

In exams, when the diagram sometimes has labels (etc. A, B, C, D) on the organelles, but the organelle is not clear, or could be one of 2, how would you best figure out which one it is?

@renegades384 2 года назад

Hi ma'am what if we have an object which has 25divisions and 26 divisions on our eyepice fit into 10 divisions on the stage what would be the real size of the object

@UmarA2203 Год назад

Hi Miss, I just had a question about the methods of studying cells part. I`ve seen quite a few questions on how to set up a slide and just wanted to ask if we should know it or not because it`s not mentioned in your full topic video or separate one?

@MissEstruchBiology Год назад

The level of detail you need for this is in my mitosis root tip squash experiment video 😊

@R20039 3 года назад

Hello Miss, how can I solve this question Calculate the calibration factor for the eyepiece gratcule at x40 (x4 objective lens) thank you for the video

@MissEstruchBiology 3 года назад

I think this is asking you to calibrate as shown in the video, I imagine the question has a micrograph image with it and a scale to calculate this?

@madhouse6613 2 года назад

Hello, at 15:56 you are explaining that the nucleus is 13 divisions long, but isn't it 13 sub divisions long. In the previous slide we deduced each division of the eyepiece graticule is 5 micrometers, so I was just wondering if the answer for the question on 15:56 isn't 65 micrometers, but a value a lot smaller due to the nucleus being ~1.3 divisions across (instead of the sub divisions). A great video though

@warmz6735 Год назад

yes exactly

@tim3898 Год назад

Hi MadHouse, Miss Estruch is correct becuase when she mentions one division, she means 1/200 If the stage micrometer is 2mm long, therefore 2000 micrometers, each division is 10 micrometers. If two divisions on eyepiece graticule fit into one division on micrometer, one division on graticule is 5 micrometers. The nucleus is 13 divisions long there around 65 micrometers. Miss Estruch is correct. Thanks :")

@garethhughes2798 7 месяцев назад

@tim3898 stage micrometers are usually 1mm in length

@Sara-sc6nm 2 года назад

HI do we have to know the apparatus and how they are used for the exam? thanks

@MissEstruchBiology 2 года назад

Hello For AQA of you look at the practical part of the specification it tells you which apparatus and techniques you need to know for all of the practicals Hope that helps

@ardisrexha868 Год назад

do we need to know exactly how SEM's and TEM's work?

@mahamn 9 месяцев назад

Do we need to know about eyepiece graticule in AQA A-level biology as it is not mentioned in the specification ?

@MissEstruchBiology 9 месяцев назад

yeah, it's on the required practical techniques section of the spec

@elliemeredith5837 2 года назад

Hello, might I just ask what exam board spec you use?

@mohid0786 2 года назад

She's AQA

@MissEstruchBiology 2 года назад

Hey Ellie, These are AQA specific 😊

@elliemeredith5837 2 года назад

@@MissEstruchBiology Oh that is a shame I am OCR - thank you though - merry christmas

@musictheme6625 Месяц назад

15:42 How come it's not 1.3 division x 5 to get 6.5?

@rebecca9558 3 месяца назад

Is eyepiece gratitude in the AQA spec

@user-vy4ol2md6d 3 месяца назад

Yes

@Sam-wi2iq Год назад

Do we need to know about calibrating an eye piece for AqA ?

@Janzoo117 Год назад

That’s what I’m saying it doesn’t seem to be in the spec

@J.246 Год назад

I’ve seen qs on them tho?

@henrycasement9101 Год назад

@@Janzoo117 its in the practical assessment section of the spec

@JonnyMagwood 3 года назад

If the stage micrometer has 100 divisions and each division is 10um then the stage micrometer is 1mm long not 2?

@MissEstruchBiology 3 года назад

Hello, Yes, well spotted, my image isn't to scale. Stage micrometers are usually 2mm long, but I haven't got an image quite to scale, and it is only showing the first 1mm. It would extend another 100 divisions to make the full 2mm.

@lewisgilfillan8679 3 года назад

How do you work out the volume in diagrams on light microscopes?

@MissEstruchBiology 3 года назад

this would be testing your geometry skills. For example, if viewing a spherical cell and you need to work out the volume then you need to measure the radius and then use the formula for the volume of a sphere. This might help ru-vid.com/video/%D0%B2%D0%B8%D0%B4%D0%B5%D0%BE-qtEpAJ7NK_w.html

@lewisgilfillan8679 3 года назад

@@MissEstruchBiology I'll give it another attempt tomorrow. Thanks