You are welcome. Here are videos for IHC staining ru-vid.com/video/%D0%B2%D0%B8%D0%B4%D0%B5%D0%BE-K1WKcbKUOMY.html ru-vid.com/video/%D0%B2%D0%B8%D0%B4%D0%B5%D0%BE-NxNEf1XSPTk.html
Hi thanks for the video. I need to count the budding yeast cells. The watershed feature won't nicely distinguish the cells that undergo the budding. Another question is that my cell looks no bigger than yours in this videol, but the area of my cells is 100 times larger
@@nrttaye4033 Thank you very much. I realize that to achieve a good watersheding, I have to have a nice thresholded pictures. I took some DIC pictures for budding yeast cells. The issue is that the interion of the cells are similar in color to the background, so when I do thresholding, the program regarded the cell interion and background the same thing, but the good thing is that the outline of the cells are clear, which can be singled out to form binary picture with cells' outline. But the issue is that some of the outline is open circled as opposed to being a closed circle. Could you please give me some suggestion to work around this issue?
Hello, sure. This might help you out to some extent ru-vid.com/video/%D0%B2%D0%B8%D0%B4%D0%B5%D0%BE-Tu9UlDspsZM.html In this video, i am measuring the cross section area, however some circles are incomplete. It can be fixed manually. Have a look between 1:30 to 2:15 minutes in the video. Select the same color of your cell outline and close it using the brush tool. All the best
Hi, please forgive me if I'm wrong but in this example you have calculated the number of cells and the area of nuclei, right? But what if I need to calculate stained area (for a given cytoplasmatic marker) and refer it to the number of cells (number of nuclei stained with DAPI)? Thank you.
Hello. The color of the nuclei can be converted either into red or B&M. This is the threshold step. Thresholding is the process of converting a color or grayscale image into a binary image. This stage generates a binary image and directs inspection to regions of interest for analysis.
Hello, i am not yet aware of any macros as of now that can measure the length of slightly round/irregular objects (nucleus shape). the closest to any automatic length measure I know is of filaments (ru-vid.com/video/%D0%B2%D0%B8%D0%B4%D0%B5%D0%BE-CP2A-Jt279c.html) and objects that are strainght and placed in either X or Y axis (ru-vid.com/video/%D0%B2%D0%B8%D0%B4%D0%B5%D0%BE-Jr2ehzIzoYQ.html). I will get back to you if I come across any macros to measure the cell length automatically.
Hello I have a question I want to count hair graft with imageJ ( i have numbers of grafts but when I use this app the results is not correct ) Would you please help me ?
hello, thanks for reaching out. Does the graft look like hairs with certain length or just transplanted with round shape structures? I may need to know how the transplant images looks like before coming to a conclusion. If they appear like cilia/filament i have video tutorials on how to count these ru-vid.com/video/%D0%B2%D0%B8%D0%B4%D0%B5%D0%BE-b3Z2t62i9Ds.html if you have overlaps then try "ridge detection" plugin github.com/thorstenwagner/ij-ridgedetection let me know if these helps. Sure happy to help.
