HPLC Chromatography Basics Explained

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(You can also watch new HPLC video with demonstration here - 👉 • HPLC Chromatography De... )
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All you need to know about the HPLC Chromatography!
HPLC(High-performance liquid chromatography) also referred as high-pressure liquid chromatography, is a technique used in analytical labs to separate, identify, and quantify each component in a mixture.HPLC separates and purifies compounds according to their polarity.
I have tried to simplify the whole process of HPLC Chromatography and explain in simple terms. Let’s look at the main components involved in HPLC in this video.
Later in this video, I discussed about Normal Phase Chromatography, Reverse Phase Chromatography and their comparison and differences.
Watch this video for more details.
Other Video Links:
ER TO GOLGI Transport: • Endoplasmic Reticulum ...
KDEL and KKXX Sequence: • KDEL and KKXX Signal, ...
If you have any doubts: Please leave a comment in the comments section below.
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Опубликовано:

2 июн 2018

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Комментарии : 2,1 тыс.

@misbahmalik9752 5 лет назад

honestly no one can teach better than you. How perfectly you explain each n everything. Truly helpful and impressive

@saydfouz1942 3 года назад

thanks a lot for making us aware about the basics of HPLC. Before meeting you, I never thought that a person could be a teacher, support, mentor and philosopher all in one! Thank you for all your hard work and dedication!

@anweshadas9611 2 года назад

12mins and the whole concept of HPLC and Reverse Phase Chromatography were understood so beautifully ❤️

@machindrasonawane559 4 года назад

I think this is one of best explanation of HPLC on RU-vid...

@mohdashafaq8230 3 года назад

Good lecture mem

@bharatambhore110 3 года назад

@science_and_fun_ 2 месяца назад

Naa bhai 😊

@shoaibsheikh5206 5 лет назад

I did B.Pharmacy but no body taught me like this during my college years. It was very difficult to understand by reading only theory. You have great knowledge mam and the way you explain is excellent. I am subscribing your channel now. I really appreciate your hard work. Thank you.

@ranjanadevi7574 3 года назад

Hi even I completed B-Pharm are you still studying?

@manisharajak6898 3 года назад

yes....An lg rha h Man k pass hee bsc MSc Or liya hota ..University prof..srf syllabus complete krne m believe krte😃😃😃

@muskanmehra9649 3 года назад

Agreed 👍

@ankurpandey2713 3 года назад

@@ranjanadevi7574 how about u? Even m doing masters in pharmaceutics

@parthsharma4081 2 года назад

Heyy m also in my final year of bpharm

@ankitamewara2649 5 лет назад

Lives simplified by your simple way of explanation . Amazing.

@anukrittimehra5681 Год назад

This video is a boon for me because I was never able to understand biophysical techniques in class and eventually came across your channel and now everything seems sorted , thank you ❤️ Ma’am your teaching style is spectacular 👍

@princesharma6166 3 года назад

Most easiest and valuable information about HPLC on RU-vid... respect to you mam 👏🏻👏🏻

@avanraws77 5 лет назад

Your lectures are very detailed and informative; you are a very good lecturer...keep up the good work!

@bhawnaa_ 3 года назад

Absolutely amazing teaching. Very proper point to point knowledge. So much helpful and accurate. ❤️❤️❤️

@incognitoburrito8 3 года назад

You're the best, you teach exactly what students need to understand unlike the rest of videos available here where they just talk bookish language

@gavsraw7812 3 года назад

Perfectly explained, I searched this content first time. Thanks.🙏🙏

@varsharani9062 3 года назад

Thank you ma'am for explaining in such an amazing way , this clear all my doubts.!! 💓

@mdsadek1424 3 года назад

I don't find any reason to dislike such an wonderful lecture

@viswanathvittaladevaram4260 3 года назад

Simple and precise explanation.Just perfect what needs to be understood about HPLC.Good informative video.

@drmahejabeen3920 4 года назад

Good explanation.... loved the video... didn’t even go back to read the theory stuff...very simplified and well explained... keep up

@sauravanand1738 5 лет назад

So simple and much effective explanation... Thank you so much

@BaaYo 5 лет назад

Hi Guys, Please watch our new videos on Mass Spectrometry ru-vid.com/video/%D0%B2%D0%B8%D0%B4%D0%B5%D0%BE-w3LS22E10Zc.html UV- vis spectroscopy links.. ru-vid.com/video/%D0%B2%D0%B8%D0%B4%D0%B5%D0%BE-1mrUHrZ-0zk.html Gas Chromatography ru-vid.com/video/%D0%B2%D0%B8%D0%B4%D0%B5%D0%BE-0KqaIKHzHE8.html and please share your valuable feedback. Thanks.

@Kapil5462 5 лет назад

U r cool.. Keep it up

@prashantrajput4465 5 лет назад

Pls make a video to calculate peak area in HPLC and how to quantify?

@BaaYo 5 лет назад

Noted. surely will make one. thanks

@avaneetyadav2066 3 года назад

Am seeing after 2 year... explained easy more than expecting .. its so simple after seeing this video .....🔥🔥

@madhurigupta8494 4 года назад

It was a very well put and precise in concept explanation, otherwise I have to run around searching for separate topics including the mechanism, principle and instrument layout

@GuptaGVlogs 4 года назад

thanks mam,,, i have not comletely understand in 8 yrs in pharma industry and by ur video i just understand in 10 min ..... now i will not forgot this in my whole life....great job..

@BaaYo 4 года назад

Thanks a lot Ashwani for kind feedback 😊

@farhanarashid3094 5 лет назад

thank you so much mam for explaining in a good way

@abusaleh9528 2 года назад

Thank You mam.I think I just want to thank you and show my gratitude because your lectures help me a lot to crack my very first interview that I faced after the completion of my post-graduation.Your tutorials are extraordinary to understand.Things would become harder without your videos.Wish your success in every steps of your life ❤️❤️❤️

@fatimarao793 4 года назад

Mene kbhi itni achi explanation nhi li kisi female teachers se u r the 1st one.... Well thanks ❤

@sonalirp21 3 года назад

The what and why for each step explained thoroughly Thank you for this amazing lecture

@brijbushantiwari5754 2 года назад

Edible.oil.testing.method

@user-pb6bc5rn9u 2 месяца назад

I am a researcher, your video helped me to understand basics of HPLC. VERY GOOD TEACHING STYLE ! KEEP IT UP.

@BaaYo 2 месяца назад

Thank You :) Happy to help

@VidhiKachadiya 2 года назад

Very good and nicely explained in a simple manner... I found HPLC difficult, but after watching this my all doubts are cleared 👍👍

@chikoogupta899 2 года назад

zindagi me pehli bar koi cheej itne ache se clear hui hai

@himanshumishra1720 4 года назад

Thank you so much ma'am 😇😇😇 it is really beneficial for me & all those students who are looking for good carrier in biochemistry.

@Bhanuprakash-pe9so 5 лет назад

Thank u so much ma'am 😊.. I was searching for this topic from long time and no one explained it in this cool manner . Firstly i thought u will not explain well but no other video explained as good as u ❤️❤️🙏. Thank u so much for your valuable time for us(students)

@BaaYo 5 лет назад

Thanks Prakash 😊

@ghulammuhammad5391 Год назад

Thank you ma'am .your teaching methods is amazing.l understood only in one seen .no one saw in my uni like you .

@dharmendrachaudharydc5909 3 года назад

You are the best discriber of instruments in my knowledge

@rameezah9404 6 лет назад

It was woow lecture simple way n easy. stay blessed mam.

@kidsofkhans6336 4 года назад

As tomorrow is my exam. . . . . But m still watching ur video as my revision. . . . . . Coz u r a best teacher. . . . . Thanks a lot for ur work. . . . . 😘😘😘😘😘

@sandeepkalita7125 2 года назад

Thank you Arti Ma'am, It's very easy to understand if the teacher taught it in the way they themself understand.

@akiakritigupta 3 года назад

Thankyou ma'am, you have explained the topic very clearly.. this video has made my concept more clearer... 😇

@BaaYo 9 месяцев назад

To access notes and practice questions, please join our Telegram channel t.me/baayo_official

@63-sayalimane7 Год назад

Mam you are saviour for us before theory exam 👍

@user-vr6ul8mk1y 7 месяцев назад

Hello Ma'am! Yesterday, I saw your HPLC video for my job interview. To be honest, I swear on the name of God, for the first time in my life I have properly learned the concept of HPLC through your video. Lots of Love from Pakistan.

@AnasKhan-up9ic 2 года назад

Your explanation is very easy and to the point, thanks for explaining

@drkiranpharmacy5114 5 лет назад

Excellent & Very Simple Clear & Nice Explanation Madam. Which is Very helped me to Understand clearly. I am Preparing for State Level M.Pharmacy Admission Entrance Exam i am also preparing for GPAT - Graduate Pharmacy Admission Test. Madam thank you very much for Preparation and Upload your Excellent Videos. These are very much useful to B.Pharmacy Students and M.Pharmacy Students who are Preparing Pharmaceutical Analysis Subject (Chemical Analysis).

@harleenkaur436 5 лет назад

Thank you ma'am Tommorow is my environmental chemistry paper and I am going to write the same .....after watching chromatography videos and this one I feel it's simpler now

@BaaYo 5 лет назад

Thanks Harleen...and all d best for tomorrow..👍

@chemistrybymirha4480 2 года назад

@@BaaYo mam aik question hai answer kijya ga How hplc differ form column chromatography

@bint-e-hawa4389 3 года назад

you are a great great teacher i learned from this video more than I did in university

@manalisingne6551 Год назад

Thank you so much for making this vid ma'am... Best ever explanation on RU-vid 😃

@arpittyagi6404 5 лет назад

Thank You so much Ma'am for such nice explanation..

@sailajamaddali3337 5 лет назад

Thank you very much for your presentation. Would like to know about the buffer you mentioned to equilibrate before the sample run (getting the base line). can you explain more about the equilibration and the buffers to use thank you!

@sindhuvelpula678 3 года назад

I have learnt so many things through ur video I got placed after watching ur videos❤️my dream has come true. Thanks alot🥳

@sagorikagogoi89 Год назад

You took my heart..M soooooo happpppy. That I found your videossss...... sooooo Thankful to you. Maam

@PrinciThapak_scienceelite 3 года назад

Your lectures helped a lot in my studies... Please keep making these videos...😊👍

@manishverma1816 2 года назад

Where are you from

@nirajsalvi9786 5 лет назад

Thank mam U are great I want to know about more vedio Like kf, gc, HPLC, and their tribal shooting In industrial use

@vishalkumarsingh6703 3 года назад

So amazing techniques of teaching Very simply delivers all concept of HPLC , Thanks 🙏

@chemtopesp.mattapally6907 2 года назад

Thank you ! you explained in a very easy understanding way.

@ferozahmedshaikh4206 5 лет назад

Thanks for making simpler I have subject about analysis in Pharm D course

@sannisaimon 5 лет назад

Thank You MAM very nice explanation.

@dnrfam8688 3 года назад

Im a big fan of you mam.u r explaining so simply each nd every topic.Ur lectures helping lot me to cover all topics

@anjalachaware742 3 года назад

Hey amazing video, explained in a very easier way. Thank you so much.

@pujakumari8787 5 лет назад

madam, in many cases multiport valve , buffer volume, pulse damper, n guard volumes are used . why?

@sushreepriyadarshanimahapa1766 2 года назад

Tq mam ...tq so much.because of uh i can cover..... my +3 final year DSE-1 papper successfully 😍love from Odisha 😊

@adarshdora475 2 месяца назад

Jai jagannath 😊

@rabiyamaqbool8649 3 года назад

you are a lifesaver, I have an exam in the morning and now I am all clear about every single detail. THANK YOU MAM

@meeraamir4423 3 года назад

Which exam?

@nitikasharma2448 3 года назад

Thank you so much Ma'am .. finally many of my doubts clear now.. 🙏

@kiransg938 3 года назад

Hi mam, I want to quantify triphenylantimony from my sample, for that which mobile phase n which column is needed?

@moni_upadhyay 5 лет назад

Thank u ma'am for giving better explanation & plz make a video on diff.types of electrophoresis ..

@udayagirimadhu7505 5 лет назад

@sagarambaliya3531 4 года назад

@SheikhLateef 2 года назад

I m watching this video again after 3 yrs. And Thanks mam. i wish i could attend ur class in front of uh ..and. i deeply love with ur style of teaching

@oliviamukherjee9901 4 года назад

Thank you very much mam. What you have taught will remain permanently in my memory.

@BaaYo 4 года назад

Thanks Olivia

@muhammadnaseer5016 5 лет назад

I'm from Pakistan your lectures are very helpful thanks Madame.

@BaaYo 5 лет назад

Thank You Naseer 😊, glad to hear that my videos are being watched in neighbouring countries 🙏🏻😊

@Impromptu21 5 лет назад

@mirhusamuddinwasif929 5 лет назад

Me also

@somalnisar6295 5 лет назад

@@BaaYo yes mam your way of teaching is very good and easy....everyone can understand it....mam kindly also mention tge name of buffer solution and mqke video on chiral chromatography 😊 respect from pak

@omprakash3456 5 лет назад

Make Vedio on Isothermal titration calorimetry (ITC) and Differential scanning calorimetry (DSC)

@zainabzara399 2 года назад

Very effective lecture ma’am thanks a lot for providing such a great info♥️

@RohitYadav-em8gf 3 года назад

Hats off to u.... You r leaving your child crying for making videos for us

@maninderrana3664 5 лет назад

Thanks mam very informative and clear plz make a video on how to standerdization hplc machine for to check diffre t component in a blood sample thanking you

@AtulKumar-gf4ve 5 лет назад

Nice

@BilalAhmad-sr5uw 5 лет назад

Mam.u r best teacher..u teach very well.and u r very pretty

@sunalipatyal9823 3 месяца назад

One of the best Explanation Ever✨

@venuy2836 3 года назад

So simply and more effective explanation

@hasanshawon1421 5 лет назад

I got my job...they asked me about HPLC ...love from bangladesh 💓💓💓

@BaaYo 5 лет назад

wow congratulations !!

@amitavsen8193 4 года назад

which company? where u come from?

@hirendevani551 5 лет назад

Mem , please make Electrometric method Videos.... Thank you.🙏

@ayeshasiddique9656 4 года назад

thank you so much.. tomorrow is my presentation , this video is very very helpful for me

@himanshupathak5501 3 года назад

The concepts about instrumentations explained by ma'am help me a lot in my examination

@Impromptu21 5 лет назад

Excellent mam

@Impromptu21 5 лет назад

@muhammadumer6571 5 лет назад

Good v good

@laxmikantvharte5628 5 лет назад

Super mam. ELISA per Video banav

@BaaYo 5 лет назад

Thanks laxmikant.. Jaldi bnaugi..:)

@jamyyy9523 3 года назад

Crisp explanation of hplc. Extremely helpful.Thank you Mam

@riteshmohanty5493 Месяц назад

What u have explained in ur vdos are best to best ....

@Impromptu21 5 лет назад

Jo ki hydrophobic the

@dr.azazahemadkureshi9848 5 лет назад

Wonderfully delivered ma'am,, I am on the edge of completion of my Doctrol studies at NIT Surat...and my entire work is on natural products chemistry... These lecture series may play a good role to improve my basics... Please share your email or linkedIn id maam!

@dr.azazahemadkureshi9848 5 лет назад

@@BaaYo thank you ma'am

@namanlohani7868 2 года назад

You are doing great job mam , you have our respect Thanking you from the bottom of my heart 🙏🙏🙏🙏

@sushantpople 2 года назад

I was very worried about my MSc Bioanalytical Science's , due to online studies i never understood the any chromatography techniques, but today only i found this channel i saw hplc video and now i am not having doubt..i will going to watch more videos, Requesting to please add Bioanalytical related topics..thankx

@biorainbows4313 5 лет назад

I wish this had english subtitles :(

@amorestperpe 5 лет назад

LMAO. I wasn't sure if she was jumping in and out of English or her accent was just that heavy. I thought she just had a heavy accent

@lovepreetkaur6746 6 лет назад

Maa'm plzzzz make A video On UV spectroscopy 🙏🙏🙏🙏🙏🙏 plz itsss request maa'm

@BaaYo 5 лет назад

Hi Lovepreet, Sorry for the delay. I was on break for two weeks, I will make video on this soon and upload it. Thanks for follow up.

@prashantjagdishkhedekar2895 5 лет назад

Ha mam uv pe banaiye jaldi

@BaaYo 5 лет назад

Hi Prashant, UV Visible Sepctroscopy video is live now. Watch it on below link: ru-vid.com/video/%D0%B2%D0%B8%D0%B4%D0%B5%D0%BE-1mrUHrZ-0zk.html

@BaaYo 5 лет назад

Hi Lovepreet, Based on your request, UV Visible Spectroscopy video is upload now :). Watch it at below link: ru-vid.com/video/%D0%B2%D0%B8%D0%B4%D0%B5%D0%BE-1mrUHrZ-0zk.html

@amuandeep1240 5 лет назад

@@BaaYo thanks a lot maa'm 🙏🙏🙏🙏🙏🙏🙏🙏🙏🙏🙏🙏🙏

@nishanttiwari8029 3 года назад

Thank you ma'am because I got job in ion health care baddi of himachal Pradesh, aap ne itna simple ho kr btaya Qa department m join kiya hun, mere pas thoda sa speak m problem h.... Mein Msc microbiology se 2015 kiya tha.... Thank alot ab mein sar utha kr chaluga thanks alot apka hr video interview prepared krne m bhut help kiya yeh meri pehli job h

@saurabhsharma5403 4 месяца назад

Wow mam ,i was in so much stress like there's so many things to study but now i have so much clearity thank you so much

@nabanitamallick9049 Год назад

Thank you so much ma'am for explaining HPLC so well.. you are great ❤

@mukeshsoni593 4 года назад

Excellent explanation, thank you for sharing the knowledge

@005abdulbasit8 2 года назад

Thanku so much ma'am I just listen you're lectures chromatography & types of chromatography before last day of my exam I gain too much point easily & my concept is clear. Thanku soooo much

@shubhangimogal8577 2 года назад

Wow mam really ap Jaise teachers ki bohot jarooorat he

@lucienearth 3 года назад

Ma'am your teaching style is Unique and very much convincing.. I became die hard fan of your videos.. Please accept my regards, Ma'am..

@aishwaryashetmandrekar6537 4 года назад

Explained very well..better than our college teachers...must needed explanation for me...thnkq ma'am

@vishwapanchal3101 Год назад

Amazing explanation mam...simply amazing of u ...u make the topic easy n short to understand. Less time is consumed for understanding a particular topic. thank you mam