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Gas chromatography | GC 

Quick Biochemistry Basics
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Gas chromatography is a chromatographic technique used for the separation of
volatile compounds. The volatile compounds are the compounds that easily gets
vaporised at room temperature. Separation occurs based on the interaction of molecules with the mobile phase and the stationary phase. The less volatile molecules interact more with the stationary phase and moves slowly
where as more volatile molecules interact more with the mobile phase and moves fast. When the sample is detected the computer gives a peak with respect to the retention time of the sample.
The area under the peak gives information about the concentration of the sample.

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21 авг 2019

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Комментарии : 181   
@prangyadas5127
@prangyadas5127 Год назад
A detailed information in a very shorter period of time and was explained in a crystal clear manner. Excellent.
@sequeira7330
@sequeira7330 6 месяцев назад
summary of the video: GC is used for the separation of volatile compounds( compounds which are easily vapourized at room temp) Components of GC: 1) SAMPLE is mixed withe a highly volatile compound like Hexane, cyclohexane, etc. • Long coiled column: can either be a packed column(made of glass/stainless steel) or Capillary column (made of fused quartz). this column is placed in a CHAMBER in order to facilitate a uniform temperature. (HIGH TEMP) During analysis, placed at 150-300°C • the SP is packed in the column. it is usually made of silicon grease or wax as it can withstand high temperature • Presence of a SEPTUM to inject sample just before the chamber - the temperature of the injection region is 20-50° higher than chamber. this is to facilitate the rapid vapourization of the molecules. 2) Mp: usually helium / nitrogen (inert/unreactive gases are used) are placed in a cylinder connected to the Column via the Molecular sieve. 3) Molecular sieve is used to filter out unwanted HCs, Water vapour, oxygen etc that may interfere with the sample during experimentation. 4) Detector
@sequeira7330
@sequeira7330 6 месяцев назад
Detector - common: FID ( FLAME IONISATION DETECTOR ) which has 3 inlets: • carrier gas from the column • hydrogen • oxygen ignitor ignited the H2 and O2 to produce a flame. when sample molecules reach the flame, they get ionized and electrons are released. across the flame, there are 2 electrodes ( cathode for cations, and anode for anions) the electrons generated are detected by the electrodes in the form of current and it is amplified by the amplifier/Computer.
@sequeira7330
@sequeira7330 6 месяцев назад
the computer then generates a graph: X axis- time y axis- current based on known samples and comparing their output with the output of the unknown samples, the retention time/concentration can be deduced
@nh-vt5ws
@nh-vt5ws 5 месяцев назад
​@@sequeira7330 seems like you're a technician of GLC... Excellent explaination bruh🎉
@pepe6666
@pepe6666 2 года назад
oh dude thank you. finally a way to understand this. the terminology of a gas chromatograph is used so much with mass spectropy that its difficult to figure out whats what. i had always thought those peaks on the chart were speaks on a spectrum! this has cleared a lot up for me, thanks.
@prayoga4419
@prayoga4419 3 года назад
Very brief and to the point. Love it. Thank you so much.
@tuffel03
@tuffel03 2 года назад
Thank you this was the exact format and presentation I needed
@sayurirathnayake48
@sayurirathnayake48 4 года назад
Thanks a very lot.i studied A to Z clearly from this video.it's amazing how you explain them simply.please make more & more videos.Good Luck
@sarahholland5980
@sarahholland5980 3 года назад
this is amazing. so simple and clear. thank you!!!!
@trevorndhlovu7834
@trevorndhlovu7834 3 года назад
COOLSOME data.It's awesome staff you arranged here.
@sarahkamau421
@sarahkamau421 4 года назад
Very well done! You're really helping us beginners! Thank you!
@mustafeabdiazizismail3771
@mustafeabdiazizismail3771 2 года назад
Thanks 🙏 to your efforts, we finally understand the different types of chromatography
@henrycalhoun1119
@henrycalhoun1119 4 года назад
thanks dude, very clear and helpful!
@sydneym3251
@sydneym3251 2 года назад
great video, great explanations, and i love your drawings/ animations!
@aga4966
@aga4966 3 года назад
Really good video, made me understand GC!
@table2790
@table2790 3 года назад
You are absolutely amazing! Thank you So much :))
@rimm2592
@rimm2592 Год назад
I like these videos. They just make my life easier. Thank you so much for the explanation!!!!!!
@Boooommerang
@Boooommerang 4 года назад
Excellent video! Thanks
@softvibes4811
@softvibes4811 Год назад
This video totally cleared everything up, thank you so much
@Carmelo12320
@Carmelo12320 2 года назад
Thank you exactly what I needed.
@hysysplusunisimandchemcad4499
@hysysplusunisimandchemcad4499 2 года назад
Excellent Work, Thumbs Up.
@fantasticcreativity6136
@fantasticcreativity6136 Год назад
O my God,,,such an amazing explanation Love it,,, clear my all doubts and confusion
@JERKINSRICE
@JERKINSRICE 3 месяца назад
Amazing! Very well explained. Thank you very much.
@bankthanadorn4598
@bankthanadorn4598 3 года назад
Well explained, better than my lecturer tbh thankss
@temmietemmieson6756
@temmietemmieson6756 2 года назад
Really well explained thank you!
@Rootfresh_channel
@Rootfresh_channel 3 года назад
Last sem exams I prepared some of difficult topics from your channel and got a 86/87 marks..
@thatvid.6628
@thatvid.6628 3 года назад
WOW! I am still repeating.
@daredevil6117
@daredevil6117 2 года назад
Wow online me itne marks.😜
@palanirajramalingam1131
@palanirajramalingam1131 4 года назад
WELL EXPLANATION EASILY UNDER STAND THANK YOU
@divya.t.george6030
@divya.t.george6030 3 года назад
very well explained understood it greatly
@shynosalexander9200
@shynosalexander9200 3 года назад
This was very helpful. Thank you :)
@triciajones6923
@triciajones6923 2 года назад
So very good.Thank you!
@hajrabarool6044
@hajrabarool6044 3 года назад
This video made my day today
@sibghatkhan3986
@sibghatkhan3986 4 месяца назад
Stupendous!! It is great effort You have make it clear as crystal 🔮 mirror
@chandrashekarn5376
@chandrashekarn5376 4 года назад
Good explanation sir with animations 🙏🙏
@wen6519
@wen6519 3 года назад
That was helpful, thank you for sharing
@AntonioAdame1
@AntonioAdame1 4 года назад
Great vid. Thank you
@rabiaameer8588
@rabiaameer8588 2 года назад
Amazing lecture clear my all ambiguous points about GC
@esterindahkusumaputr
@esterindahkusumaputr 8 месяцев назад
Thank you for your video, it's clear
@themoreyouknow585
@themoreyouknow585 3 года назад
Amazing I really appreciate it
@faizakainat9427
@faizakainat9427 3 года назад
impressive..beautifully explained
@arbazchaudhary5752
@arbazchaudhary5752 Год назад
thanks for this video you explained very well
@stellalesirite4059
@stellalesirite4059 3 года назад
Thank you for this🙏
@zahidhussainzaigham9223
@zahidhussainzaigham9223 Год назад
Well explained briefly. Thanks
@mdraselmiah3403
@mdraselmiah3403 4 года назад
good explanation and it will help me more..thanks
@jono2167
@jono2167 3 года назад
Excellent video. Very clear and informative. Good job mate!
@satyasurya8923
@satyasurya8923 2 года назад
Thank uuu sooo much for this wonderful explanation 👏👏👏
@abrarrakin1757
@abrarrakin1757 4 года назад
superb man, thanks a lot
@ianedmonds9191
@ianedmonds9191 3 года назад
Excellent. Thanks for that. Luv and Peace.
@Ara2061
@Ara2061 4 года назад
thank you.. .thank you... thank you
@imtme
@imtme 2 года назад
thank u, u are so helpful, more helpful than my lecturer
@mustakim2144
@mustakim2144 3 года назад
Thank you!
@user-yt2yl3fn4z
@user-yt2yl3fn4z 8 месяцев назад
Respect 🙏 from Africa Zambia better than my lecturer for a year thanks I know love this course❤
@sabahedayati4474
@sabahedayati4474 22 дня назад
Thank you🌱💙
@niazali5861
@niazali5861 Год назад
Respect from Pakistan 🇵🇰🇵🇰🇵🇰
@MrKpy86
@MrKpy86 2 года назад
I work for a company in the Chemistry industry that manufactures instrument and that was pretty accurate, good job. Only thing is inlet is at 20-50C higher than the max temperature reached by the GC and there is also an inlet of Makeup Gas for the FID which is usually to compensate the fact that nitrogen or helium coming from the column is usually much lower than the other gases. Other than that, great video
@kamnabharati2970
@kamnabharati2970 2 года назад
Thank you Sir.❤️🙏🏻
@shourovhossain8498
@shourovhossain8498 2 года назад
Amazing.....the video clear the concept of chromatography.
@mariofelipequevedolozano3659
@mariofelipequevedolozano3659 3 года назад
Thanks a lot!!!
@_sri_702
@_sri_702 2 года назад
Thank you 👍
@carmempacificodasilva4799
@carmempacificodasilva4799 Год назад
thank you so much!!!
@bijiteshchoudhury8274
@bijiteshchoudhury8274 2 года назад
Well explained Thank you
@BrainKhan
@BrainKhan 3 года назад
Nice one explanation i always found there
@nargesmaqsudi62
@nargesmaqsudi62 4 года назад
Thanks a lot
@dimitrikolotauri6622
@dimitrikolotauri6622 4 года назад
thank you sir.!
@ValeriaGobjila
@ValeriaGobjila Год назад
thank you for this informational video on behalf of all science students :)
@halimzaini1796
@halimzaini1796 4 года назад
Thank you
@9madhumitha928
@9madhumitha928 2 года назад
Very useful ☺️👍 thank you
@user-dq9ty5tq1z
@user-dq9ty5tq1z 2 года назад
thank you sir for your good explaination.
@dinosanchez8528
@dinosanchez8528 3 года назад
Good video, thanks
@user-wt9gv3kd3q
@user-wt9gv3kd3q 6 месяцев назад
Thank you!!!!!
@bhaveshthakor4219
@bhaveshthakor4219 6 месяцев назад
Amazing explanation
@user-jd3bk2wq6m
@user-jd3bk2wq6m 2 года назад
In bad need for applications of gas chromatography 🥺💔
@yasminarijah1646
@yasminarijah1646 Год назад
Thank you. Very helpful
@mumizashahid6047
@mumizashahid6047 2 года назад
Very very very very helpful Thanks alot for this
@EnglishExcel2208
@EnglishExcel2208 4 года назад
Super xplanation
@baochau4877
@baochau4877 Год назад
thsnk you so much, very helpful
@sujikumar7831
@sujikumar7831 4 года назад
Super..very useful.. simple
@jignasachiramana8269
@jignasachiramana8269 2 года назад
really helpful !
@juliana_jesus
@juliana_jesus 2 года назад
Thank you very much!! May I have the references for further reading please?
@hisanathasni5499
@hisanathasni5499 3 года назад
Thanks bro ❤
@nihalanasrin9598
@nihalanasrin9598 4 года назад
Highly useful
@jayliu7786
@jayliu7786 2 года назад
i have a organic chemistry lab tonight and hoping that it goes well. thanks for the video, it's very insightful :)
@muhammadammar6262
@muhammadammar6262 3 года назад
very well done video very good keep it brother
@seeker8906
@seeker8906 4 года назад
Thank you Sir
@Matteo-mx1sz
@Matteo-mx1sz 3 года назад
thanks
@ankitagaonkar4298
@ankitagaonkar4298 4 года назад
Wow explained well
@CaulderChristian
@CaulderChristian 2 месяца назад
the tography part of the word is pronounced like photography hope that helps
@aminechahid2004
@aminechahid2004 4 года назад
THANK YOU
@j.d.tgaming7199
@j.d.tgaming7199 3 года назад
Hiee
@mairaseiilgazy7510
@mairaseiilgazy7510 3 года назад
Thanks
@laylachemist5245
@laylachemist5245 4 года назад
Well done sir
@user-kt3xu3ml1q
@user-kt3xu3ml1q 2 года назад
It's really helpful ...thanks 💜
@praveenkumarkp4084
@praveenkumarkp4084 3 года назад
Very helpful 👍 plz do classes of HPLC and spectrophotometer
@seripahnyambura2782
@seripahnyambura2782 3 года назад
Thankyou
@venkatk669
@venkatk669 3 месяца назад
Good information
@muskanagarwal1173
@muskanagarwal1173 3 года назад
Amazing sirr
@ianmatthewkline8279
@ianmatthewkline8279 2 года назад
Okay but what about when two samples have similar or even the same retention time? I use LC-MS/MS as a main tool for my PhD work but I was curious about how this other technique works.
@1987Wulf
@1987Wulf 2 года назад
You can use a different phase to achieve different RTs. There are some that will never work on GC. For instance, M,P-xylenes are not chromatographically separable. However, using a HRMS will differentiate the identifications.
@pharmasolutionpakistan7668
@pharmasolutionpakistan7668 2 года назад
Good explaination
@b4baigan989
@b4baigan989 10 месяцев назад
Wow... Just wow
@w.a6369
@w.a6369 2 года назад
Well explaination
@kooky434
@kooky434 8 месяцев назад
Amazing :D
@amitgupta-yr5pl
@amitgupta-yr5pl Год назад
Great present
@sanjayg114
@sanjayg114 Год назад
In fid carrier gas was nitrogen/ helium which carries the sample to burning area?
@khushbujha4957
@khushbujha4957 4 года назад
As we can say in 6min our separate sample is there??
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