yo' Bro. you don't know how much your videos have helped me to study for my molecular biology tests. I visit yoursite whenever I have a doubt. Thanks a lot.
Thank you so much Professor, it was fully packed with the clarity I was hunting for !! May God bless you abundantly for sharing your knowledge freely !!
Such an interesting and detailed video. I study Pharmacy and this will definately help me at Pharmaceutical Biotechnology's exam. I would like to give you an idea for another video: cosmids used as vectors, concatamer creation and the help of the enzyme terminase for the packaging at phage λ heads. Keep spreading your endless knowledge!
This was so amazing! Thanks a million for all the hard work you put in! Your explanation was precise and neat and perfect! i'm really grateful :) Sir do you have a video on lac operon? Please do share the link
Are we supposed to remove the genes involved in the lytic cycle of the phage for the purpose of cloning our DNA into E.coli cells? Is the vector then still viable for the gene to be inserted into E.coli DNA?
hello!, I have a question.. at the end you say that we insert the fragment of recombinant DNA into the lambda phage, but where does it come from (the lambda phage)? has there been previously transcription and translation of phage DNA to make the capsid, if yes, so we need the material necessary for transcription / translation in the medium, it would therefore be necessary to add DNA polymerase, dNTPs and ribosomes, right?
yes, but if I want to get the proteins which the inserted gene codes for, won't the viral dna have to be transcribed and translated within the host cell? In that case, does it really matter if the phage follows the lysogenic or the lytic cycle? (that is, if it gets incorporated OR replicates right away, in which case i suppose it will work much like a plasmid?). Are the terms lysogenic - lytic cycle even used in this case? Is there any danger for the host cell either way? OR is the phage used as a vector only in the case I want to create a genome library, not a cDNA library, so I only want it to go lysogenic? (genome library--> just study the genome, cDNA --> to receive polypeptide chains)
This is really helpful. may I ask, what is the advantage of lambda vector compare to viral vectors? Does it allow bigger DNA fragments (e.g. 1Mb) to be inserted?
The DNA inside the phages having 3 segments,if the segment 2 is taken out and our gene of interest is located in case of this the proteins which are produced by the segment can't produce inside the virus.Is this can't create any issues???
Yes, you need restriction enzyme digestion to cut between fragment 1 and 3, leaving 2 without attaching to the sequence, so how do you remove 2 before inserting the target DNA?
