Wonderful explanations. It is unbelievable how young you are and how well you know what you teach, apart from having excellent teaching skills. The world would be so different if teachers like you were teaching in our classrooms. Thanks for dedicating your life to this, thank you for helping us see things in science clearer, thank you !
Maybe you cover this in a future lecture but you have glossed over the main difference between pBR322 and pUC18, and that is how you determine which cells have taken up a recombinant plasmid (as apposed to taking up just the starting plasmid). In pBR322 you must use replica plating to determine which cells have taken up recombinant plasmid vs which cells have taken up the starting plasmid. For example, if you were cloning into the tetracycline gene, you would first select with ampicillin in order to identify which cells took up the pBR322 plasmid. You would then check the ampicillin colonies to determine which ones were no longer tetracycline resistant. Ampicillin resistant and tetracycline sensitive cells will have the recombinant plasmid. If the cells are resistant to both antibiotics, then they took up non recombinant pBR322. With pUC18 you can tell in a single step which colonies took up recombinant plasmid because these cells will be ampicillin resistant but will not turn blue because the inserted sequence has disrupted the B-gal. pUC18 has several other advantages over pBR322 but the ability to screen for recombinant plasmid in one step instead of two is a major advantage.
I just want you to know that you're awesome. Every time I happen to stumble upon one of your videos, my comprehension level immediately goes through the roof. Thank you.
I must say a big thank you. you've helped through med school, with your lectures that are so easy to understand. i can literally listen to you and enter into the hall with pride and the results are always very fruitful. thank you so much for your lectures. it has helped me a lot.
Do you know how much I love you, everytime I have a Bio test I come to you, and once the video is done, I understand that topic like the back of my hand. Thanks so much :)
I'm french and you really did help me with my homeworks. Your explanations are really easy to understand and it's clear enough in just one sentence. You saved me ! Thanks !
Your videos are always of such high quality and grade it amazes me how knowledgeable you are! What is your occupation and job? I'm dying to know. Are you a teacher? Or are you doing this as a hobby.
Really clear explanations. Constant repetitions makes it really easy to follow and to retain the information. Love this teaching method, keep it up! Was able to watch at 2.3x and the pronunciation was still crystal clear!
