LGC's KASP Genotyping Chemistry Explained: The KASP reaction 

KASP is a PCR-based genotyping chemistry suitable for genotyping SNPs and InDels. KASP has been used to genotype millions of samples worldwide. If you have you ever wondered “How does KASP work?”, this series of three videos explains the KASP reagents, the genotyping reaction and how the data is analysed. This is the second video and shows what happens when you run a KASP genotyping reaction.
The example shown in this animation is a SNP genotyping KASP reaction. It gives a step-by-step explanation of the early rounds of the PCR and then goes on to explain how the alleles present in the DNA sample will result in a fluorescent single from the completed KASP reaction.
This video demonstrates how the KASP genotyping reaction would report DNA samples that are homozygous or heterozygous for the target SNP.
After the reaction is completed, and the fluorescence has been measured, it is necessary to analyse the date. Please watch the next video to see how this is done.
Learn more about KASP genotyping at www.lgcgroup.co...
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