Making and Using a Spore Syringe (for mycology)

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To get access to ALL CONTENT or request a topic, please use patreon (my content is FREE I don't use paywalls, support only if you feel you have benefitted and can afford to do so), I also post and interact with people there: / gordotek
Before asking a question, please read the FAQ: / 22774475
Social media:
/ tekgordo
/ gordo.tek.33
[Note: If you use any of my Amazon links it helps support the channel! (“As an Amazon Associate I earn from qualifying purchases.”) I make zero from RU-vid. Thanks!]
amzn.to/3ABkdmP (Sterile Syringes, luer lock)
amzn.to/3g7wVQP (optional syringe tip caps, luer lock)
amzn.to/3IOdFUu (Lids)
amzn.to/35zcPgf (mycobags)
amzn.to/3IPr5Q7 (presto sterilizer)
midwestgrowkits.referralrock.... Look under “Substrate Jars”
Whole Oats: www.tractorsupply.com/tsc/pro...
Or on amazon for much higher price: amzn.to/3rT0NWk
amzn.to/34hnITg (Polyfill)
amzn.to/3KYHcN8 (16 oz mason jars)
amzn.to/3gaPYtt (hygrometer/thermometer)
I show how to make and use a still air box in this vid:
/ 29675383

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Опубликовано:

10 июл 2024

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Комментарии : 125

@filippoarlenghi6247 8 месяцев назад

The acquarium heater idea is super clever

@sherrypayton6871 Месяц назад

Great ideas! I’ve watched a lot of videos and I have been in this hobby for over a year, but I didn’t know about the horse grain. Thank you.

@xj11w2c7 Год назад

Yo dude thank you so much for letting me know about getting syringes from tractor supply. I'm working really hard not to support Amazon and just been looking for syringes all over

@shareeverar9420 Год назад

There are so many incredibly detailed steps, thank you so much for this informative video. Very thoughtful and thorough.

@kwahdomo9598 2 года назад

You are the man Gordo 🙂

@user-by4xy4ed6i Год назад

Glad I found you! I'm just getting started and you are full of great information

@smokedaddy773 10 месяцев назад

This video has been amazing g and so much help. Thank you for the detail

@toddwmac Год назад

Thanks man....really appreciate your contributions.

@starchylde4538 Год назад

Ty fir your knowledge!! Awesome channel!

@myotheraccount5947 Год назад

Tractor supply.... you must be nearby me! I'm new to mycology and it seems I've gotten way too much poor information from RU-vid channels. You're video here makes things much simpler than others and gives me hope. I'd pay you for an in person consultation if you're close enough and willing

@gordotek Год назад

Private message me on patreon.

@derby99ultra Год назад

I've been really struggling to find a reputable source for my first spore print in the uk. I'm gonna take a peep at that patreon now. Thanks gordo.

@gordotek Год назад

You are on the right path ;)

@profetarmageddon 2 года назад

I appreciate your information!!!

@nathanw5747 5 месяцев назад

instead of the acquarium heater . you can also use a plant seedling heating mat + a PID temperature controller . vivosun has the mat + temp controller for like 25$. i happen to have all this i grow superhot peppers as a hobby . so if you do not have fish this maybe a option with dual use .

@MyTube4Utoo Год назад

Fantastic! Thank You.

@GeeZOhio 2 года назад

Awesome video

@user-uc9ku2gj6o 7 месяцев назад

THANK YOU

@pieterplant926 Год назад

Awesome!

@stevenamgad2339 8 месяцев назад

Thanks

@timothykramer1856 Год назад

Thanks mush❤

@Lemonz1989 9 месяцев назад

A tip for sealing unicorn filter bags if you don't have an impulse sealer: I use the seal function (without vacuuming) on my cheap vacuum sealer machine. It works fine. :) I don't know how much an impulse sealer costs in the US, but here in Denmark they can be really expensive - some cost hundreds of dollars.

@gordotek 8 месяцев назад

Good idea. FYI you can get an impulse sealer on Temu for $20

@lanthanumlanthanium6373 7 месяцев назад

@@gordotek Don't support that country

@csucsucsucsuka8356 2 года назад

Love you men! Superb.content

@bruhmomente7324 Год назад

Hi Gordo!, quick question, I have multi spore syringes, & was wondering what the best course of action to duplicate them would be?, should I just transfer to agar & isolate, then inoculate to grain to get a spore print?, or just use the isolated agar to make liquid culture? Much thanks

@bruhmomente7324 Год назад

Also, would CVG with calcium hydroxide work for panaeolus cyanescens, and do they require special agar, or would malt agar work?. furthermore will a liquid culture comprised of 500ml purified distilled water, 20gs 1:1 dextrose, golden malt, & 5gs peptone be a good liquid culture mix for panaeolus cyanescens?

@gordotek Год назад

You can either direct inject to sterile grains or put the spores to agar, let it grow out, then transfer that to grains. Then apply the grains to substrate and grow it out to obtain more spores from mature fruit bodies. You can also use liquid cultures if you want, I don't like LC personally, easily contaminates and you can't always see that it is contaminated.

@recyclingnik 2 года назад

Thank you for the good and informative video! How long would you cook de hulled polished barley grains? I have those at hand..

@gordotek 2 года назад

Sometimes the hulled versions don't work very well, they become mushy fast. But you can give it a try. Maybe just pressure cook them for 30-40minutes.

@recyclingnik 2 года назад

@@gordotek yeah that`s what happend ! it worked somehow and the growth was more aggressiv in the jar but breaking it appart was a pain because it was mushy and sticky..won`t recommend hulled grains

@Eddielongan 7 месяцев назад

Question,I'm having trouble understanding or answering by watching videos...... after you boil your grain, strain then sterilize, is the grain completely dry when you put it in jars and add your spores,or is it wet?

@gordotek 7 месяцев назад

So, the grain itself absorbs water, it will look all "puffed up", there is lots of water inside, but not that much water on the outside. It shouldn't look very wet, for example there shouldn't be a puddle of water on the bottom of the jar. When you inject the spore water that will provide just enough extra water to get the spores germinating.

@vincentwu2848 Год назад

Thanks for sharing such an informative video! Out of curiosity, is it a bad idea to wash the spores off of the foil instead of scraping it off with the scalpel?

@gordotek Год назад

You can do it that way or even form the foil print into a cup and add water directly to the print, swirling and scraping it up with the syringe needle if you want (don't poke through though!)

@vincentwu2848 Год назад

@@gordotek Oooh thanks for the advice, I've never done prints before so I have no idea what to expect. I figured if its powder on a sheet of foil, squirting water from a syringe would wash it right off but based on your feedback it sounds like it will take some agitation with the needle to get the spores off. I use 2oz condiment cups for agar, do you think it would be a good idea to try to take spore prints with a condiment cup instead of foil? Then I can place a lid on the cup after the spores drop, and then inject water through the lid and draw up spore solution..?

@420Bill 2 года назад

10:40 you might be able to use a hair straightener to seal the bag as well

@gordotek 2 года назад

I've heard of people using a hot iron, or even heating up a metal coat hanger on a stove and using that to seal it (probably not a great idea).

@jimmcdees1341 11 месяцев назад

Bro I wish I could afford to become a patron atm! I'd help support a few of our family! I'm just a single dad with custody of my youngest daughter and do it on my own! It's why it's taking me so long to get into the mycology... Everything is expensive 😂 mush love man! I'd love to know how to get a spore print without being a patron

@gordotek 11 месяцев назад

Well its only $2 to join and I give spores to all patrons so that would be one way to get spores for next to nothing ;) I wish you well in your situation, I imagine that is quite challenging 🙏❤

@dustinkeller4595 Год назад

I just have to say ty.

@ricardokjf 2 месяца назад

Hey loving you videos an way of explaining this all, first class. I'm very new to this, so was wondering if that free spore sample is still available??

@gordotek 2 месяца назад

I used to give prints to anyone that asked but I've been overwhelmed and can't keep up anymore, sent out more than 2,000 prints, so I had to cut back, but I STILL give free prints to all patrons at any tier (as little as $2 this basically just covers postage). I'm actually sending out two prints now to every patron, and they are arguably the best genetics in the known world. You can't be that. I just pay it forward and hope enough people will appreciate the content and stick around to make it worth doing. Also note there is a community chat now on both the patreon app and website and the GordoTEK chat has been super active with everyone helping each other out, great vibe.

@gordotek 2 месяца назад

www.patreon.com/GordoTEK is the link for patreon

@jessicastacy211 2 года назад

Do you have a video on how to make Changa? I prefer Change to crystals. Thanks in advance!

@gordotek 2 года назад

Its very harsh on the lungs and even the guy that invented it says he no longer uses it, haha. I don't recommend it but its very simple, just combine the DMT and MAOI containing plants and smoke.

@dentaldickwad Год назад

Tha ks again Gordo!! Why can't we transfer direct from the print into agar??

@gordotek Год назад

You certainly CAN I have a separate vid showing how to make and use agar. That is actually my preferred method generally unless my goal is speed.

@sydneywalker2966 Год назад

What gauge needle is recommended if you don't buy spore syringes specifically?

@gordotek Год назад

Pretty much any guage will work, even big blunt tips work. The spores are tiny and can pass through any size needle. The tractor supply syringes are 22 guage.

@christoffergustafsson3057 Год назад

I would like a spore print 😊

@plamenconev3387 6 месяцев назад

hello friend, one print in how many milliliters of water is added?Also how many milliliters are injected in a liter jar? thanks in advance

@gordotek 5 месяцев назад

You can get away with as little as 0.5cc of spore solution if you shake it up good and ensure there are spores flying around just before injecting, but most of the time you want to do at least 2 or 3 injections so that would be 1 to 1.5cc minimum per jar (doesn't matter the jar size). You should see growth in the jar after putting in incubation temps for about 3-5 days, once it colonizes about 1/3 of the jar you can shake it up and spread the mycelium everywhere, then it will rapidly colonize all the grain (only do the shake up one time).

@plamenconev3387 6 месяцев назад

Hello, I have a very important question. I am considering using the following ingredients: Rye grain, parrot seed mix, brown rice flour, vermiculite. My question is this: Will it be successful after colonizing my jar (possibly, I would hope: ) then directly transfer the colonized substrate to the fruiting chamber and add only - first layer of perlite, middle layer of mycelium, upper layer of vemiculite. Are these nutrients enough? thanks in advance

@gordotek Месяц назад

That might work, you should check out my patreon page for lots more info and Q&A plus an active community chat where people help each other out, its all FREE.

@IvanLopez-ed4ti 8 месяцев назад

Do you have a video of what to do once you begin the mushroom colonization?

@gordotek 8 месяцев назад

Yes seem my FAQ link is in the description.

@gordotek 8 месяцев назад

www.patreon.com/posts/22774475

@Clyffcarlock1 Год назад

How much water in the pressure cooker? Where would you put the cup of water to be sterilized?

@gordotek Год назад

Follow the directions for your cooker, the quantity depends on the size, but generally there will be some removable platform that you put the items you want to sterilyze on top of, and the water fills up to just below that platform.

@UGSETH2 6 месяцев назад

Could the aquarium heater be used throughout the entire growing period? I loved the idea and can't see why it couldn't be :) Peace

@gordotek 5 месяцев назад

Yes it can.

@benink5690 Год назад

So the print isn't contamed? Anyway to take a sterile spore print?

@gordotek Год назад

If the print was prepared in front of a flow hood, its likely very clean, I rarely see contams coming from prints although its possible. If the person who made the print didn't use a flow hood, its very likely full of contamination.

@SconnerStudios Год назад

My only criticism is you are a little quiet. Your videos are really helpful.

@gordotek Год назад

Noted! ;)

@nickfranco962 Год назад

Is it dangerous to put that bag in on the top? Say if it breaks or opens up and clogs the steam outlet hole.. Can't that make a bomb?

@gordotek Год назад

Most pressure cookers have backup relief valves, but you do not want anything touching the steam vent. Some people put metal rings or other things on top to ensure nothing can clog it.

@zacm4740 2 года назад

I remember Gordo one of the 1st if not the 1st to show how to grow mushroom in bulk like 5 years ago then Joe Rogan started talking about dmt then Gordo did tell using wood chips then Cris cantelmo did a video and mentioned Gordo's tek Gordo even left him a message in his comment section then Cris went crazy and capped himself poor guy. I remember though Gordo I remember I was watching it as it all went down you taught me a lot what happened to your videos on mush and dmt?

@gordotek 2 года назад

Greetings, the good news is that all of my original content is still available, follow the link in my vid description to the FAQ, at the top there are links to the old content that was banned. Cantelmo was a sad story of mental illness, some actually think he was murdered but I doubt that. The craziest thing about his story is that he had been trying to contact the FBI about a conspiracy to infect the world with a dangerous pathogen, this was literally just a couple of months BEFORE the covid pandemic began! Ah well, wish he could have found the mental health help he needed. For what its worth, I advised him to STOP USING ALL psychedelics and to seek some help but I could not get through to him. He was clearly abusing DMT which I do not condone in any way.

@gordotek 2 года назад

www.patreon.com/posts/22774475

@thederpguidetolife3014 10 месяцев назад

If I don’t have a pressure cooker what can I use? And how?

@gordotek 8 месяцев назад

You can pasteurize things in a pot of boiling water, just requires much more time and is less reliable.

@Aqua1111-sm3qx Год назад

"Wouldn't there be any contamination issues for a spore print to pass from the United States to Japan? I'm curious if it's possible to make a syringe from a spore print that comes in a zipper bag and is shipped in a box...

@Aqua1111-sm3qx Год назад

"All preparations have been made, but it seems impossible for me to bring this to Japan..."

@gordotek Год назад

No, I've actually sent several prints to Japan (and all over the world to more than 70 countries) with no problems. Mail to Japan is even very fast too. If you want prints contact me on patreon.

@Aqua1111-sm3qx Год назад

@@gordotekgod's grace...

@Aqua1111-sm3qx Год назад

@@gordotek "How long can foil-wrapped spores remain contaminated and unusable?"

@girththeogre 3 месяца назад

Thank you for a great video! Very informative!! PS: Subbed!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!

@lbar2458 Год назад

Uhh i would like a spore print. I ordered a syringe but it seems to have more contams than spores.

@gordotek Год назад

I used to give them to anyone that asked but I've been overwhelmed and can't keep up anymore, sent out more than 1000 prints, so I had to cut back, but I STILL give free prints to all patrons at any tier (as little as $2 this basically just covers postage). Giving out the prints I set a world record with and won the Cultivar Cup with).

@anthonyroman218 7 месяцев назад

May I please chime in regarding your laminar flow hood, so I noticed you have the inoculation set to Hight correctly within the correct parameters of your laminar flow except for one thing is that you have your inoculation site on a upside down Pyrex plate, which does not allow proper air pass through through the bottom and underneath of the Pyrex plate , you have placed upside down, which may result in a upward draft of your laminar flow. may I suggest a wired basket turned upside down instead of using a solid glass Pyrex, which may introduce a upward draft of turbulent air from the bottom part of your laminar flow setup remember turbulence is always your worst enemy when it comes to laminar flow I also noticed you have Foil that has been stuffed between the filter itself and your inoculation site on the upside down Pyrex plate to reduce this updraft so it is apparent that you are attempting to address this to a certain extent, but just to be on the safe side, I would rather use a wire basket and adhesive glued to a piece of glass or even some acrylic or something that is flat that you can glue on top of the wire, upside down basket to create a solid platform to work on and to remain free of worry of any updraft because of the wire basket will allow air pass through and even better no need to worry about stuffing the foil between the pyrex plate and your filter anymore🙃🙂🙃😎

@gordotek 7 месяцев назад

You are very observant, and absolutely correct! I actually have a separate video on building your own laminar flow box where I do a better job, and use a proper wire rack, haha. But that laminar flow box in this vid is a massive commercial unit that is 2x4 feet in size, the sheer size and flowrate pretty much ensure no matter how silly my platform is, its unlikely to have a problem. But still, you are correct, I should be a better example.

@smokedaddy773 10 месяцев назад

Is it to late to get the free spore print sir?

@gordotek 10 месяцев назад

No I'm still sending out prints. Just message me on patreon: patreon.com/GordoTEK

@juliansullivan102 Год назад

Would love a spore print

@gordotek Год назад

Send mailing address by private message.

@juliansullivan102 Год назад

@@gordotek where will I find your email address?

@lucaripoli2717 Год назад

If you leave it in jar it will produce sclerotia?

@gordotek Год назад

Only certain cultivars will do that, I am not a fan of this type of grow as the sclerotia are slow to grow and "dirty" when harvested, usually harder to grow, just seems annoying to me with no particular benefit other than some weird legal loophole in the Netherlands where sclerotia get special protected legal status for inexplicable reasons, haha.

@nickfranco2259 11 месяцев назад

Do you sterilize those Luer locks?

@gordotek 10 месяцев назад

You can indeed if you need to. They should come sterile at least for the first time use, but yea I sterilize and reuse them.

@dustinkeller4595 Год назад

Roseburg

@justinturnbull5466 Год назад

would love a spore print how do i message you

@gordotek Год назад

Best way is on patreon, private message.

@Bob-gn8ph Год назад

You are awesome Gordo.. Jesus God bless you:-) Luke 21:36 KJV:-)

@urbanforager90 Год назад

I want a spore print please

@gordotek Год назад

contact me on patreon

@amarmeriout7707 10 месяцев назад

Hi man your content helped me very mach after lining about psychedelic I desided to start growing them but unfortunately I live in Algeria were there are no spors around I would really appreciate if you helped me to get some I believe in psychedelics and Im rady to do whatever it takes to make this work kan you help me to get some spors in exchange I'm ready to do what ever it takes

@gordotek 10 месяцев назад

Just contact me on patreon: patreon.com/GordoTEK

@ninjashrine0292 7 месяцев назад

is it too late for a free spore print???🙃

@gordotek 7 месяцев назад

I am still giving away free prints to all patrons, its as little as $2 to join and that barely covers my costs. There should be a message icon on the patreon app and on their website, put GordoTEK in the to box and I should get it. Or click on my name and then there should be a message link.

@urbanforager90 Год назад

How to

@serdarguler2340 Год назад

Sory 🙏 Do you sell Psilocybin mushroom spores?

@gordotek Год назад

No, but I give away free prints to all patrons at any tier level.

@obasi_12 Год назад

I will refer you to an online psychedelic store where you will get Psilocybin mushroom spores,dmt,lsd chocolate bars edibles!!

@obasi_12 Год назад

sam_trippz!!

@obasi_12 Год назад

On insta

@serdarguler2340 Год назад

@@gordotek Thank you for your interest

@docphillips3291 Год назад

🍄🍄🍄

@yt7927 10 месяцев назад

Only 3 things in life I want. A Lamborghini, A beautiful woman with a spectacular ass, and a spore-print from Gordo!

@aldovarela3234 2 года назад

Print?

@gordotek 2 года назад

Contact me on patreon, FB, IG, Reddit, or Twitter

@duggdrealer Год назад

Whole Oat is the worst grain to use. And n it takes rhe longest .

@gordotek Год назад

I seem to get about the same colonization times regardless of species of grain. Maybe you just had weak spores or some other problem (too wet or too dry) in the instance that gave you that conclusion?

@Anonymous-ru2wk Год назад

Gordo means fatty in Spanish FYI

@jpinksntc 2 года назад

@gordotek Год назад

contact me for free prints

@johnbauby6612 Год назад

WHere are all these low prices coming from? I bought a bag of oats at TS last week for double the price you published. Could they have doubled in a year or is it the state you live in?

@gordotek Год назад

You can search for a graph of the price of oats, what happened was actually pretty amazing, yes the price actually went up 200% in a year but has since fallen back down quite a lot this year. But with inflation in general, prices of everything will probably keep creeping up every year (hopefully not as much as oats have!)