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Measuring Microbial Mutation Rates with the Fluctuation Assay - a 2 minute Preview of the Experimental Protocol
Rok Krašovec, Huw Richards, Guillaume Gomez, Danna R. Gifford, Adrien Mazoyer, Christopher G. Knight
The University of Manchester, School of Biological Sciences; University of Waikato, School of Science; The University of Manchester, School of Natural Sciences; Université du Québec à Montréal, Department of Mathematics;
Here, a protocol is presented to perform a fluctuation assay and estimate microbial mutation rate using phenotypic markers. This protocol will enable researchers to assay mutations in diverse microbes and environments, determining how genotype and ecological context affect spontaneous mutation rates.
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26 авг 2024
