After taking tons of Chemistry, Biology and Physics classes while attending college 50 years ago, I am drawn to your excellent lectures on a myriad of topics. You, sir, are an excellent instructor. Wish I had one like you those many years ago. I will watch every one of your videos just for the joy of it before dementia sets in.
I am SO GRATEFUL for this lesson. I'm an RN who recently switched to Oncology nursing a short while ago. I've scoured the internet and specifically RU-vid for an adequate explanation of monoclonal antibodies. I have been unable to find one until encountering yours. Thank you, and keep up the good work!
Thanks a lot for your videos! You are speaking quite clear so it is not really difficult to understand you for non-native speakers as I am) I hope I will learn a lot from your channel. I'm currently a 3rd year student of St.-Petersburg State Chemical-Pharmaceutical Academy, so your lectures are useful for my studies. Thanks again, keep going
Wow! I love this lecture about turning normal cells into cancer cells for study..wish you could come over to The University of Nairobi for some lectures on Immunology..! This is a blessed effort sir.
Even though I was majored in Biochemistry 20something years ago, I really forgot most of what I learned because I pursued organic chemistry in graduate school. Now I have to study biochem again because of my job and your lecture helped me alot. Thank u
Hi, so can the monoclonal antibody be purified directly after extracted from the body (not the cells), the same as with polyclonal antibodies as you explained in the previous one? if not, why? Thanks
It's an honor to write to you Cause you are really Awesome in teaching every single aspect and I gotta say if you wasn't I may have not been accepted to the Biology Olympiad So thank you soo much It will be Much more Awesome if We could have all (most)of ahat you said as a PDF or sth to print out and study and review.Every video takes me a lot to write down the most important things that need to be reviewed but all you say is to a large dgree important Thank you sooo much and I had a Question I'm a Biology Olympian and I wanted to know till this part of biochemistry is there any tests or Questions you suggest Cause I will only kearn and remember sth if I put it to work myself anything that I can somehow test my Understanding and See how much I remember Thank you sooooo much For your Awesome job
Wow fantastic job in simplifying this subject of Monoclonal treatment had no idea what great potential this treatment is this treatment will be a great revolution in medicine let’s hope it can help with Covid-19
Great !! thank you so much .I have a question .. do you know how scientist use monoclonal antibodies in Prostate-specific Antigen test (PSA) if you know can you just briefly explain to me how ! ( I mean how they're using the antibodies to measure this antigen )
Thank you - I still didn't understand one thing : after creating hybrid cancer-plasma cells, we still have a mixture of antibodies against different epitopes - how do you create monoclonal antibodies from the hybride cells ?
If we have the sequence of the protein(the antibody), using a virus or some other method, inject the genetic sequence of the protein into any bacteria. Then the bacteria will produce these anibodies for us.
Quick question, the hybridoma should have 4N chromosomes (2N from normal, 2N from tumor ), when these hybridoma mitosis, what will happen? Its progenitors will have 2N chromosomes? If so, if its progenitor split again, will it still have the ancestor all the hybridoma characteristics?
This video is not essential for learn to formation of mAbs becoz he did not explain the selective media and HGPRT knockout gene in Multiple Myloma cells. I think as per full understanding of hybridoma technology these things are very important for us.
sry what happens when we frezze a Cancer cell?! it is still alive continues dividing but slower?!So we can have it anytime we needed it right?That's why we Freeze those Cancerous plasma Cells
