No-pour Agar Method 

Build a still air box. I've done hundreds of transfers with very low contam rate.

What Kyle said. SAB is the way to go.

You could do unmodified SAB like 90second mycology thats what I use

I'm trying it with half pint mason jars atm with that thought in mind. I've never used a port to inject liquid onto agar. To your other point, as far as i know, agar is generally not air tight when using parafilm or Glad wrap. And it's definitely not being kept airtight in ketchup cups. I've heard Saran wrap does not let agar breathe enough, and therefore you should avoid it as a potential wrap. With that in mind, I'm probably going to leave the lids slightly less than 100% tight.

It is really the agar that benefits from cold water...it lets it hydrate and decreases clumping and clouding. split your water, cool 1/2 and add agar, stir on and off for 15 minutes. Then add the rest of your water and dry ingredients, stir and cool

I assume you mean the 2 piece tops that are for canning? If you don't have a hole in the top of the metal, you should flip the lid and leave the screw top just slightly looser than hand tight. This however will defeat the purpose of the no pour technique because you'll have to take the lid off and flip it again, thus contaminating your sterile agar.

ge not necessary with such small cultures.

@@apex.amatuer You definitely want some gas exchange. Even regular petri dishes are vented. It also appears he has a small hole in the top of these lids.

It appears that he has a small hole in these lids, but I may be wrong. What you should do instead is make a hole in the lid and cover it with micropore tape or an adhesive filter disc.

@@DangerRussDayZ6533They don’t need any. Not sure where you’re getting this info from.

Only if the petri dishes are made out of borosilicate glass that can be autoclaved, or made out of PP5 (polypropylene). Regular petri dishes are typically made out of plastic that cannot withstand high temperatures and will melt.

Wally world sells em in packs of 8. Go to canning supply isle. 👍🍄 Don't get the gray ones stick to the white ones. Gray deforms easy

He did boil it

You bring it up to a boil to help mix the ingredients together. Agar needs higher temperatures to dissolve in the water completely.

I'm trying this today, and was wondering how difficult it might be to see condensation on the white lids. My agar is usually pretty clear, so I'm hoping to see what's going on through the bottom of the jar. They're going at 15 psi right now

@@ariannaclearday did your agar come out good?