Pouring agar plates, recipes and a few tips for sterile technique. 

It isn't too hard. Some will inevitably get contaminated. Don't worry. It's the door to all other advanced techniques.

That sounds great, but the agar wouldn't be sterile. Better to measure with accurate scales, a known recipe, and consistent ingredients.

It is very soothing until you pour over 100 plates :)

@@edwardgrand Ahhh The dilemma of turning a passion into a job , Trust me I get it. It’s a fine balance between the two. You still got it ! 👊🏻💯

I've got a latex sponsor ;) LOL Too bad I only need the gloves :(

Just getting into home mycology and it is incredible that soneone would EVER want to put gloveless hands on anything that is supposed to be sterile or sanitized!!

Thank you for watching. I still miss Michigan sometimes. Especially this time of year.

Personal, time and financial choices to consider. Different for everybody.

I have redundancies everywhere.

@@edwardgrand ahh ok

So many Michigan people in Mycology. What happened to us? Alexander Smith, Hesler. Great mycologists! Now we're carrying on the tradition in a slightly different way :)

Sorry to hear that. I don't understand the no glove faction. Some kind of machismo, ego thing?

@@edwardgrand Thanks but I knew better, but now I really, really no better. :) Great marathon stream the other day. 9 hrs., wow! Good thing for CoPilot's summary feature. It is great when you and D. Keys get together. In appreciation.

I absolutely believe in wearing gloves. I can't argue the use at all, definitely a good things to do. I can't stand gloves on my hands and never use them. I'm not trying to promote anything at all. I simply can't tolerate them for more than a minute or two. I frequently pour 100 plates a week and have done thousands in my life. No flow hood, just an oven with the rack sitting on top and the door of the oven open with the oven on so hot air is moving up. A lot of alcohol frequently. 2 to 5 out of the 100 batch get contaminated. Frequently only 1 to 2 but occasionally 3 to 5. Good work in this video.

I like to stack the deck in my favor.@@ZCRAIZED1

@@ZCRAIZED1I've had similar good luck with oven tek (hundreds not thousands for me) albeit with gloves. It is a simple approach more people could use, If I can ask, how do you store your poured plates? With no FFU, no way I can get a sterile bag over them so I roll the dice with the original bag with pretty good luck. Also, any thoughts on vented vs. non-vented plates? I'm seeing edge lately and was wondering if vents are contributing to it.

FOR 1X RECIPE (500 mL) 4g Gellan Gum (low acyl) 6g LME (light malt extract) 0.5g Nutritional Yeast 0.5g MgSO4*7H20 (Epsom Salt) 0.05g Dye

OMG. I don't even want to do that calculation. It is easily in the 10,000s, maybe 100,000s. I used to use 6 cm plates in grad school and I stored them on sheet baking trays and rollers like they use in bakeries. AHHHH. PTSD!

The sterile air is coming from above. No worries. Non-pathogenic fungi. Limited work area.

Lots of cool dye degrading enzymes in fungi.

Morons...you'd be surprised how much some people hate gloves...mostly dirty hippies. LOL

@@edwardgrand fuckin hippies, they are in no short supply in this space.. i just wish they would use deodorant and the chicks would shave... I think i would give them a pass on the gloves if we could just get those 2 boxes checked

Up to you ;) some people don't wash their hands after 💩 :)

The best!

Mostly I just do it for record keeping. And I like pretty colors. If one plate in that color batch is bad, I can check the others quickly that are the same color. I found out today yellow isn't the greatest though...not enough contrast.

Thank you for the lovely comment about starting your day. That makes my day!

GRYA recipe 2% Glutinous Rice/Whole wheat flour 2% Agar 0.1% Baker's or Nutritional Yeast Food Coloring (if desired) FOR 500 ml Total volume 10 g Glutinous Rice/Whole wheat flour 10 g Agar 0.5 g Baker's Yeast Food Coloring or 1g charcoal if desired. 500 ml tap water - Should make between 20-25 standard size plates.

@@edwardgrand the rice flour that’s just ground up flour? I’m definitely trying this? The rice says glutinous rice on the package?

@@edwardgrand what’s up edward I couldn’t find where it says glutinous rice at the store but can I use brown rice flour? Whole wheat flour is whole wheat rice?

@@edwardgrand does it hurt to add light corn syrup to the mix?

@@Houseofmycology I wouldn't.

The fungi don't really care what they look like :) Lucky for me too! I'm not the prettiest specimen.

I like aluminum foil 'lids'. Easy to get a 'seal' that still allows air in and out to equalize the pressure.

Yes. Skin flakes and other detritus are all over your body. I try to always wear gloves when handling plates. It is a simple/cheap way to prevent extra vectors of contamination.

No particular reason for using a flask. Sometimes, I use a media bottle or even a liquor bottle. Whatever you use, make sure it fits in your pressure cooker (PC). Vertical and horizontal hoods/FFU are the same idea. Doesn't matter which you use. Thanks for the watching :)

Denatured ethanol is cheaper here that isopropanol. It's not the kind of ethanol you want to add to a drink...trust me...me and a friend tried. Works the same as iso. Slightly more volatile and flammable.

@@edwardgrand how much you paying per gallon of ethanol?

@@jerodyoumans6002 $4

@@edwardgrand that’s pretty good. Right now I can get it for $1.00 until the current supply runs out.

@@jerodyoumans6002 You should start selling that as automobile fuel!

Do you feel better now?

@@edwardgrandDoubtful

Too funny! I do the exact same thing all the time. If you don't like what you are watching, change the channel! But either way, shut up!