Thank you so much!!! Your explanation really clarified a lot of tinny doubts that I was afraid to ask to any of my teachers! But you explained all so well and soo clear!!! Thank you so much!
I love the way she explains everything very simple and clear.I wish she was my professor.She inspires me alot! I just wish I was working as her assistant.
I was feeling dumb today at class ´cause I couldn´t imagine how the dna strands were located, now everything it´s crystal clear, thank you. You have beautiful eyes btw =)
Ma'am...the way you explained this is simply amazing, you have no idea how much insight I got watching this video. Please, any chance you could do another video on how to analyze the primers designed using DNAMANN or NCBI just to make sure all the primer design criteria are met?...I'll really appreciate that.
Thank you for your help. The beginning of your explanation is what made it clear for me , when you delineated the 5 to 3 directions of the primers, because from there, it is easy to see which complement strand is necessary. Brava
Dear teacher. I admire your scientific mind and the decency which you radiate. I seriously invite you in Greece for scientific discussion and vacation, the land where western science was born. I am involved in diseases and laboratories. Will be honor to meet you in person. I wish you the best and thank You so much for the informative excellent video.
@Amalia safiee - Only the sense strand or plus strand of DNA is shown in that example and the forward primer in this case is elongating the other strand .
PCR needs a double strand, which is why it requires DNA or cDNA. If you put a forward and reverse primer on the same strand if anything were produced it wouldn't be functional. Remember the overall goal of PCR is to get a lot of replications of your DNA.
This is a good video but it got me a little confused. Forward primer is compliment of template strand and Reverse Primer is reverse compliment of template strand right?. The reverse primer you showed at 7:45 makes perfect sense but the forward primer you should earlier for the same example is identical to the sequence. How does that make sense? The strand that is always shown, sometimes only, is 5 to 3 so isn't the primer annealing to that strand AND therefore must be compliment? Could someone tell me what I am overlooking.
Monsterchief300 Hi, the forward primer binds to the strand which usually isn't shown (3' to 5'), and because it is complimentary to the strand it binds to, it is the same as the top 5' to 3' section. The reverse primer binds to the strand usually shown (5' to 3'), so it is complimentary to that strand, but backwards.
I finally get it now. I was focusing too much on the top or 5' to 3' strand, thinking the primer pair annealed only to this. Knowing this now il try to clear some confusion I had with coding direction given on NCBI and designing primers on Ape.
One thing when designing primers, what if you already have start and stop codons at the ends of the templates? I start and ATG AND TGA IS already there. Do i disregard it?
If I got your question correctly, the forward primer which goes from 5' to the 3' direction, bind to the single strand DNA that goes from the 3' to the 5' direction.
