Excellent description of the two methods. It would be useful to compare images of the same specimen using the two methods to see what difference there are in resolving power for the respective methods. When do you use phase contrast in preference to DIC? Thanks
Something doesn’t seem correct with that Bright Field demonstration 🤔 I’m fairly new to this, but if you’re using a 10x objective, then it seems like there should still have been SOMETHING that showed up in an unstained image (someone correct me if I’m wrong). It looked almost as if the image wasn’t adjusted for bright field during the condenser change , but if the condenser was changed, wouldn’t the lighting, camera exposure settings, and possibly even the focus need to be adjusted as well?
What do you use to clean your microscope? I just purchased an old Zeiss Axio Vert 100 and Objectives for my first ever microscope and love it 😊, but have found it quite difficult to get it clean and keep it clean. Also, tried cleaning the mirrors under the eyepieces and not sure if I did more harm or good... I put it all together from 2-3 RU-vid videos and no history using a microscope, with DIC and phase contrasting... I also purchased a Varel condenser/objective that I haven’t tried yet, and fluorescence DAPI/TRITC filters that I haven’t installed, which is when I’ll probably finally read the manual for safety reasons lol
