Plant Micropropagation -101 「Orchid Tissue Culture」 

Thank you for sharing! I am amaze for such a young age and so passionate about your work. Please post the updates on tissue culture

Thank you for upload by the way, it's always good to learn from the mistage. I wish I could learn this stuff when I was young as you.

Hey I did this a while back when I did my training. We followed an exact protocol by a commercial grower and none of us had any success, even though we followed the instructions to the last detail. With sterilization you only kill of bacteria, fungi, viruses and viroids on the surface of the explant. If there are fungi and bacteria growing within the plant you have little chance of a successfull in vitro culture. All you can do is transfer the infected explants again and again, sterilizing in between. Also, the smaller your explant, the higher your chance of success, since there is less material for fungi and bacteria to hold onto. Where I work we often use shoot meristems, sometimes only a fraction of a millimeter in size. This can prevent contamination. You can also try to be more and more agressive with your sterilisation, use longer and longer sterilisation times and stronger solutions. Hope this helps, keep on trying!

Thank you very much ✌️😍

I congratulate you, although you are quite young, you have succeeded in a job that is quite difficult and requires knowledge. A big like from me.

Hello can you give a components of tissue culture?

That's a very practical video. Can you also please tell us the contents of the medium?

Plz make video on anthurium tissue culture

Your channel is great! Thanks for sharing this info. Regards from chile 👍

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I want to learn how to do that can you gave me the school

How are you sterilizing the jars. Also, I don’t see an IPA spray bottle in the enclosure. How are you sterilizing the enclosure after you seal it?

Hello, your medium what are,the thinngs you put inside how Manny gram agar, water,enz

So how can you take the gene that creates ultra violet colors and introduce that DNA into a plant so that it glows in the dark? Using at home plant tissue culture.

would love to know your media chemical composition

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Whats the name of the medium?

By 'dehydrated water' you presumably mean distilled?

At 3:45 are you saying "and *don't* use the bleach solution that ..."? The mumbling speech gets in the way a bit, I had to replay a few other sections over and over too before I could determine what you said :(

wow so inspiring

Super nice video! A few questions: 1. Which medium have you used? 2. Does boiling everything in the pressure cooker ruin something? 3. Did you use phyto hormones? I hope you continue to upload videos

You see the rip in the glove right? I bet you didnt have a big enough fan also

I m also a scientist .....doing job at tissue culture lab ...... contamination grow always how I removed it

What plants did you succeed with? None of them look like orchids.

4:53 Your knife is too blunt.

You shouldn't cut a scalpal towards your hand like that. Cut away from you.

kurang steril prosedurnya..tumbuh jamur

Not brand new tech, been used for 100 years

Tissue Culture was developed in 1895. Definitely not a new technology

You audio is not consistent, low and high. Put life to your voice.