The Future of Houseplants: Tissue Culture (TC)

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TIMESTAMPS:
0:00 Intro to Tissue Culture
0:07 What is Plant Tissue Culture?
0:22 Micropropagation is a style of Plant Tissue Culture
0:30 Tissue Culture Process
0:51 What is MS Nutrient Media (Murashige and Skoog )
0:55 Plant Tissue Culture Multiplication Recipe
1:01 What is Subculturing
1:05 Multiplication Phase of Tissue Culture
1:07 Rooting Phase of Tissue Culture
1:18 Acclimation of Tissue Culture Plants
1:22 Tissue Culture Lab Setup
1:26 Laminar Flow Hood for Tissue Culture
1:31 What is the purpose of a Laminar Flow Hood for Tissue Culture
1:40 Tools for Tissue Culture
1:51 Thai Constellation Monstera in Tissue Culture
2:23 HEPA filter for Tissue Culture Lab
2:35 Tissue Culture Demonstration
2:47 Remove unneeded biomass from the explant
3:00 Subculturing for micropropagation
3:50 Sterilizing epipremnum explants
4:35 Sterilizing tools for tissue culture
4:52 Placing explants in multiplication media
5:13 Multiplication of Thai Constellation Monstera
5:40 What is Subculturing
5:52 What finished tissue culture looks like
6:00 Acclimation process for tissue culture
6:20 Plants that are grown from Tissue Culture (TC)
6:40 Advantages of Tissue Culture
6:50 Disadvantages of Tissue Culture
6:55 Rot on Tissue Culture Monstera Thai Constellation
7:05 Advantages of Tissue Culture with Genetic Modification
7:23 How Tissue Culture helps preserve genetics for future generations
7:52 Please Subscribe to the Channel :)
In this video we go over:
What is Tissue Culture
Tissue Culture Explained
The History of Tissue Culture
Micropropagation
Tissue Culture in Houseplants
What is TC
What does TC Mean?
#TissueCulture #ThaiConstellation #RarePlants
Music from Pond5
Stock footage provided by Freepik, downloaded from videvo.net
Other stock footage by from videvo.net
I do earn a small amount money from amazon affiliate links if purchases are made, but with NO extra cost to you. This does not create incentive to make videos like this. My goal is to make reliable, honest, plant content viewers can trust. -Chris

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Опубликовано:

30 июл 2024

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Комментарии : 89

@alimartinez1298 Год назад

This is fascinating! Thank you for the information!!

@rj6940 5 месяцев назад

Thank you for sharing the knowledge. Blessings to you 🙏🏻

@northernplantuckyplantcinc2630 Год назад

I think this may be my favorite video you've done so far❤🎉

@prettyingreen Год назад

Aww thank you!!! I tried something a little different this week -- glad to hear you enjoyed :)

@anushaudayakumar7929 4 месяца назад

I'm A/l agri student so this video is a very important for me.And i like this video .thank u so much❤❤

@prettyingreen 4 месяца назад

Glad to hear that 🙌

@rosemontez3674 Год назад

Thank you for sharing! What an amazing concept. Good job on this video and detailed explanation!

@prettyingreen Год назад

Ahhhh thank you Rose!!

@renatecormick7399 Год назад

Amazing, thank you so much for sharing this information, I enjoy learning about TC and the future of using it.

@prettyingreen Год назад

Absolutely! I enjoyed making this video 😄 More like it to come!

@THOTHvii Год назад

Wow, so I have watched about 8 videos pertaining to this subject and I wish I would have just started with this one. This is a very well put together video. I really like your brevity and concise instruction. I think TC is going to be my new hobby. Thanks for sharing the video.

@prettyingreen Год назад

Glad you enjoyed it! TC is really fun and challenging :)

@karenfreedman9180 Год назад

I’ve always wondered… Thanks for sharing!❤️

@prettyingreen Год назад

Absolutely! 😄

@jamiedelgado1484 Год назад

What a great video 👍

@queeniefelixjoiedomantay1913 Год назад

Hello . I love your videos. Im really interested to learn more about tissue culture. All the tools and compounds needed. I really want to try it at home.

@KimSol90 Год назад

This is truly incredible indeed.

@prettyingreen Год назад

It is! Big fan of TC

@donnawest-newman956 Год назад

This is great information, thanks for dumbing this down. So interesting

@prettyingreen Год назад

So glad you enjoyed!! 😄

@Lisa-gs7wj 7 месяцев назад

It's nice to see how you explain everything about TC. I myself working on Plant tissue culture where I have to induce callus. I have used reported PGR for callusing but it seems like any hormone and its concentration has no effect on my leaf explant which is concerning. Also I'm using both young and mature leaves. What could be a reason for my leaf explant to not give any response on any callus inducing PGR? If you could answer my query?

@nelsonmayor9300 7 месяцев назад

excellent video, thanks for sharing. What antioxidant do you use for monstera? I have had serious problems with oxidation. Thanks

@ChadGardenSinLA Год назад

I can't express enough how much into the science I am with this hobby! Thank you for making this video. I'm curious to know if the protocols you used are generally available online for "common" plants like pothos. I understand why they are a well guarded secret, but I've seen a lot of small nurseries here in California (as well as instagrammers) performing tissue culture in their back rooms. So it makes me wonder how readily available (or shared) certain protocols are seeing so many hoods outside of a hospital or lab setting.

@prettyingreen Год назад

I'm so with you on this, Chad! TC is what really tickles my brain in the plant world. I find it fascinating! I think there are tons of protocols that are good starting points available online. In my four years of experience playing with TC, I've found each species, and potentially sub-species, needs its own curated recipe for that EXACT plant. This can be tough because I usually make 1 Liter of Multiplication media at a time, and that fills 20-30 vessels. So I usually just put Monstera, Philo, Epi, all in the generic Multiplication Media I've made for that day. But I would say I change the mix almost every time I make it!

@ChadGardenSinLA Год назад

@@prettyingreen Thanks for the tips! I'm not jumping into tissue culture just yet, but if I do I may want to experiment with a Pink Pineapple, or other patented plants like the Philo Gabby.

@alienperfection973 Год назад

I can understand your concerns.. I am trained for TC, I work in the biotechnology field, and yes protocols are well guarded because it's intellectual property. But if you understand how it works an learn the basics, it's not that complicated :) Personnaly I prefer not to do this at home because the quality of the materials and equipements can cause issues... and of course knowing how to manipulate under a BSC requires some training. I think it's best to leave this kind of stuff to people that really know what they are doing and have real lab conditions 😅

@alienperfection973 Год назад

@@aroidaddiction I have Biotechnology diploma 🙂 bio tech is a very wide field and used for much things TC is biotechnology !

@Encrypt- 7 месяцев назад

@@alienperfection973 i'll do what i want thanks, easy to find studies with protocols

@saraanderson4029 Год назад

When in the process do you usually transfer your tissue cultures into semi-hydro environment vrs soil?

@Mario23558 Год назад

Very informative .🎉

@prettyingreen Год назад

🤗

@greenhut1642 11 месяцев назад

Is it possible to make plant media at home, what ingredients are in growing medium. Possible to assist me sir

@user-xv2sr5jo4l Год назад

I'm a pharmacy technician by trade and I specifically work in a sterile admixtures lab where I utilize aseptic technique to prepare drugs for intravenous use. I find the process of tissue culturing really fascinating and I kind of want to try it myself since it seems to fall in my lane. I'm a little curious about your setup, though, if you don't mind some questions. In a typical laminar flow hood, the air flow is in sheets (hence "laminar flow") from a singular direction. I could be totally wrong, but I don't think that can be achieved with the type of filter you're using. We also utilize PPE including (but definitely not limited to) sterile gloves to avoid introducing potential pathogens. I know I'm working with more sensitive mixtures, considering their administration, but do these practices not impact your process? I'm legitimately curious because I'd like to see if this is something I could set up at home and while a fully sterile environment would be impractical, I could probably rig something up if it doesn't need to be 100%!

@chitsu1388 8 месяцев назад

You can make an environment sterile enough by using a still air box, I'm sure his flow hood is fine :D

@lindsey4178 Год назад

could someone answer a question for me? I understand the concept of tissue culture BUT how is it any different from propagation aside from the sterile environment and the more nutrient rich growth medium. I just wonder, if the cutting and everything is all the same, is the only difference a more controlled environment? This may be a stupid question. Lol. I'm just trying to understand exactly how it works and why it's preferred to regular propagation? Especially with plants that aren't too difficult to propagate to begin with. EDIT....maybe I should have finished the video first. Lol. I think I understand a bit better. This was a much more in depth explaination than other videos I've seen. Very interesting

@ChadGardenSinLA Год назад

Undifferentiated cells AKA stem cells, exists in the plants structure, like the stem. "Cuttings" typically have one node from which one plant can grow out from. But with the activation of stem cells, multiple plants can grow from one stem. The protocols (aka the growth medium mix) can activate stem cells to grow whatever we want. The initial phase are leaves (to create new clones) second phase is the activation of the roots.

@lindsey4178 Год назад

@@ChadGardenSinLA thank you! I think I wasn't realizing that the cutting wasn't only growing normally from the node in a perfect environment but also multiplying quickly due to the stem cells. This video and your comment explained all of this much better than other videos I have watched about it.

@carolstuff Год назад

This is so interesting. It’s not easy, but hopefully more TC will enable me to get a Thai less expensive. Thanks so much for your work on TC & for sharing!

@prettyingreen Год назад

This is exactly the beauty and speed TC provides over typical stem propagation!

@lindsey4178 Год назад

@@prettyingreenOK....ive come back to this comment because I was looking for someone who knows about tissue culture and can maybe help me with a plant problem I'm having. Basically, I had an apparently healthy plant that rotted quickly and I'm wondering if it could be related to TC. I'llstry to keep it short but give the important details. About a month ago I ordered an N'joy pothos. I got it the next week and it looked beautiful. I let it just sit for a couple days but then repotted it because it was just in a soggy coir mix. The roots looked good....white, maybe a bit thin, but healthy for sure. Each individual plant (I think there were 6) were all crowded in the very center of the pot and there were a LOT of little sprouts, too small to identify as pothos or not. I separated, repotted, and after about a week I noticed they weren't perking up the way I expected. I tugged on the plants and almost ALL of them just came right out and were black and rotted under the soil. Could this possibly be because it wasn't hardened off after TC? And could those sprouts have been because it was still a bit exposed to the hormones and making babies? I don't think it could be the soil because I potted the pothos with another plant and it is just as happy as ever. It also couldn't be over watering. If it were pests or already rotted, I would have seen the evidence when removing the old soil....thanks so much if you see this LONG comment! I'm just trying to figure it out and I'm hoping I don't need to worry about my adansonii (the plant it was potted with) . Could it be because it wasn't hardened off properly? Also, if it's important I got it on amazon from California tropicals.

@miciglaric Год назад

Is plant also cleaned from viruses and diseases which are inside plant and does't allow plant to give full potential? Does tissue cultured plant have bigger yield?

@greenhut1642 2 месяца назад

Possible to helpe in tissue culture, my medium get contaminated every time

@mohan9670 5 месяцев назад

Is that possible to do tissue culture on elephant foot yam?

@user-bc1fx4qu1q Год назад

What kind of jelly did you use inside the bottle

@beanerb822 Год назад

Agar agar? I think it's a type of gelatin like seaweed

@queeniefelixjoiedomantay1913 Год назад

Same question here

@mahadihasanrahul9848 4 месяца назад

It’s agar agar @@queeniefelixjoiedomantay1913

@amberfrazier575 3 месяца назад

@@beanerb822yes but with plant food and other hormones. This process would be hard to replicate in a home due to the lack of a sterile environment.

@plantypittsburgh Год назад

I am really looking forward to the future tissue culture videos. I harbor delusions of my own little lab some day. Another video topic that you might want to cover is light & variagation. I see conflicting advice and in my experience when increasing light should improve variagation, it does not. (I'm looking at you, cebu blue ghost). I've never had a plant revert. How does variagation happen? How is it replicated in tc and when can it not be? So many questions!

@prettyingreen Год назад

I think it's very much possible to create an "affordable" tissue lab for

@plantypittsburgh Год назад

@@prettyingreen I have a lot of questions. 😆 Thank you for the informative videos!

@khalidsultan7641 Год назад

A beautiful and wonderful channel, but I have a question. How long does plant tissue culture take until the seedlings are ready for sale? Thank you

@markeastridge9649 Год назад

Can be a long time if one is proving their clones as stable, healthy and hardy for long life in normal conditions before distributing to the public. Short answer is maybe 3 months from plant cutting to sell question mark plants in dirt with minimal acclimatization.

@celgenbiotech 5 месяцев назад

Do tou sell culture bottle of Albo variegated

@manjuk1708 Год назад

I think economically it makes sense to use tc for producing more monstera albo or Thai plants rather than golden pothos.

@prettyingreen Год назад

Correct! I was using a Golden Pothos in the video bc it likely will have contamination due to me talking through the entire video without the laminar flow hood on, and handling it incorrectly

@greenhut1642 4 месяца назад

How many hours light and AC must be on for tissue culture plants

@prettyingreen 4 месяца назад

Lights are usually 16H on. Temps around 76f

@BikramchaudharyB 10 месяцев назад

When we select part of plant for tissue culture it's called explant not mother plant

@prettyingreen 10 месяцев назад

Correct. I guess once it’s severed from the mother plant, it becomes an “explant”

@pennymayo2332 Год назад

Interesting

@prettyingreen Год назад

😄

@jackfang58 Год назад

What is your sterile solution? I'm trying to replicate

@madisontaff7379 Год назад

How do I get into this for a possible future job?

@LarsLarsen77 6 месяцев назад

Move to a country where labor is basically worthless and apply for the job.

@user-lf7gl8ob8u 4 месяца назад

That is not producing laminar flow my good sir other then that great video

@jack_irl 3 месяца назад

would you not wear a mask and gloves to avoid breathing on the TC?

@prettyingreen 3 месяца назад

Absolutely wear mask. Gloves are dependent on style of technician

@bikexploration 8 месяцев назад

The only reason I Don't do TC is of weak plants. It's more like quantity vs quality. And I'm more into quality.

@prettyingreen 8 месяцев назад

I’ve found TC doesn’t necessarily breed weak plants. It can at times due to somalclonal variation from repeated sub culturing, but that can also result in positive benefits too.

@LarsLarsen77 6 месяцев назад

TC makes plants more vigorous, at least temporarily.

@jacobhicks7319 Год назад

6:14 those roots are JUICY

@prettyingreen Год назад

Yessssss 😄🤗

@wazif4464 Год назад

wow very nice video👍 from my fren farhan safuddin

@Strong_Tomato_ 5 месяцев назад

i think calling it the future of houseplants is a bit misleading because its been around for quite a while now(like 30+ years i think), nice video tho

@prettyingreen 5 месяцев назад

True!

@notgojo491 Год назад

so, basically the ,,you can make a new plant from every part of the plant, even from a piece of leaf" part is bs and you literaly propagate the plant by node, just on a fancy way

@LarsLarsen77 6 месяцев назад

You can make another plant out of ANY cell of a plant. Even a single cell. It's just harder.

@LarsLarsen77 Год назад

That's not a laminar flow hood.

@prettyingreen Год назад

It's MY laminar flow hood: en.wikipedia.org/wiki/Laminar_flow

@jordough4495 Год назад

What he's saying is, your filter is not producing a laminar flow of air (which it's not), which is the sole purpose of a "laminar flow" hood

@LarsLarsen77 8 месяцев назад

It's not laminar flow. Also, you shouldn't use a magnetic stirrer for sterilizing explants, the magnet can damage cells and cause somatic mutations.@@prettyingreen