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Plant Tissue Culturing in the Bathroom?! | Subculturing with Francisco

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Ever thought your bathroom could double as a lab? 💡Join Francisco, our talented Lab Director, as he takes science out of the conventional lab setting and brings it into... the bathroom! 🚽 Use this link to save 10% off your first purchase: www.plantcelltechnology.com/?...
In this one-of-a-kind experiment, Francisco demonstrates that you can achieve plant tissue culturing from virtually anywhere with a little innovation and some basic kitchen equipment.
It's not just about doing science-it's about breaking barriers and showing that you don't need a high-tech lab to dive deep into the wonders of plant biology. From your kitchen to your bathroom, the world is your experimental playground!
🔬 Missed our last video? If you want a comprehensive guide on how to prepare plant tissue culture media, no matter your experience level, make sure to check it out: • How To Make Plant Tiss...
Like, Share & Subscribe for more unconventional science adventures! And remember, think outside the lab. 🌱🛁
🔗 Timestamps:
0:01 Intro
0:10 Plant Tissue Culturing in The Bathroom?! Without a Laminar Flow Hood
1:33 HEPA Filter with UV Light
1:53 The Materials We Are Going to Use
6:01 Setting up my Bathroom Station to Plant Tissue Culture
13.50 Subculturing in the Bathroom Heliamphora minor
17.40 Pinguicula
20.30 Darlingtonia Californica
26.20 Venus flytrap
32.38 Conclusion
38:00 Outro
🛒 Products We Used in the Video:
- Supreme-Grade Agar: bit.ly/3T4nZy3
- MS Media w/ Vitamins: bit.ly/3YPAaAp
- PPM™: bit.ly/3ZLhXES
- Gellan Gum: bit.ly/421HWto
- IBA Solution: bit.ly/3ZysQKA
- NAA Solution: bit.ly/3LsMP9p
- BAP Solution: www.plantcelltechnology.com/b...
- TDZ Solution: www.plantcelltechnology.com/t...
- Kinetin Solution: www.plantcelltechnology.com/k...
- Square Media Vessels: bit.ly/3J7yigg
- Round Snap-Lock Containers: www.plantcelltechnology.com/r...
- Biocouplers™: bit.ly/3J3aD0r
- Biocoupler™ Glass Set: bit.ly/3mHm6v3
- Biocoupler™ Bundle: bit.ly/3Jui3eR
- Laminar Flow Hood: www.plantcelltechnology.com/2...
🎓 Exclusive Master Class Alert! After this tutorial, take your skills to the next level with our comprehensive Tissue Culture Master Class. Dive deeper into advanced techniques and hands-on sessions to become a tissue culture maestro! Register at plantcelltechnology.com/master-classes/
👍 If you found this tutorial helpful, give us a thumbs up, share with your fellow plant enthusiasts, and drop a comment with your experience and questions!
🔔 Subscribe to our Channel for more expert-guided plant tissue tutorials and insights!
🌱 Follow us on social media!
INSTAGRAM: / plant.cell.technology
FACEBOOK: / plantcelltechnology
LINKEDIN: / plant-cell-technology-...
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#PlantTissueCulture #tissueculture #PlantCellTechnology #tissueculturemedia

Опубликовано:

27 сен 2023

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Комментарии : 38

@rebekahstahl1018 10 месяцев назад

This is perfect timing!!! Thank you so much❤

@PlantCellTechnology 10 месяцев назад

Thank You!

@sweettooth5737 10 месяцев назад

This weekend will be the final setup of my home TC setup. 🎉 Pennsylvania winters are my biggest worry; last winter we had -40°F wind chill and everything in my house froze. 😕🥶 Pray for a warm winter, please! 🙏

@Lilyastacio 10 месяцев назад

Hi Francisco! Is the first time watching you! I am here to learn! Thanks for sharing your knowledge!!!

@PlantCellTechnology 10 месяцев назад

Hi Lily, Hope the shared information is useful for you. Thanks for your comment.

@briansplantlife 10 месяцев назад

Nice presentation!

@PlantCellTechnology 10 месяцев назад

Thank You!

@nadunrosh 5 месяцев назад

wow thats cool

@danieljheelan5256 10 месяцев назад

Thanks a lot for sharing Francisco. Take care while working with flame, cleaning and tools in alcohol. I put fire inside. Also the flame risk to melt the top of the plastic container. Daniel

@PlantCellTechnology 10 месяцев назад

Thank you for the concern, Daniel!

@mikearts6836 10 месяцев назад

How come the media jars you use is not hte one you sell on your shop?

@hbee368 3 месяца назад

Your bathroom bigger than my apartment 😂😂❤

@PlantCellTechnology 3 месяца назад

Haha!!

@pangalang 10 месяцев назад

thanks for your great work. i have 2 grams of meta toplin and need to know how to make a stock concentration and the best solvent to use can meta topolin be autoclaved? Can meta topolin concentration be stored over time?

@PlantCellTechnology 10 месяцев назад

You can learn about preparing stock solutions for plant hormones here: www.youtube.com/@PlantCellTechnology/search?query=plant%20hormone Add meta topolin in the media after it's cooled down using the syringe filter technique.

@buckbeaksgarden761 6 месяцев назад

great questions! I want to know now too :)

@Unkn0wn1133 10 месяцев назад

Do you also do flasking orchid seeds or just tissue culture plants

@PlantCellTechnology 10 месяцев назад

Currently, we are just doing tissue culturing of plants. Is there anything specific in which you are interested?

@Unkn0wn1133 10 месяцев назад

@@PlantCellTechnology orchid tissue culture?

@PlantCellTechnology 10 месяцев назад

We didn't try orchids in our lab. But, you can easily find the protocol for the plant on the internet as it's one of the extensive tissue-cultured plants. @@Unkn0wn1133

@labeenausmani9696 10 месяцев назад

Please tell me the Hardening process

@sweettooth5737 10 месяцев назад

There are a bunch of vids on YT that teach acclimatization for TC. In my opinion, having a good humidity dome with vents is the best way to start.

@sweettooth5737 10 месяцев назад

This is what I use: ru-vid.com/video/%D0%B2%D0%B8%D0%B4%D0%B5%D0%BE-L97ZFZ56pg4.html

@PlantCellTechnology 10 месяцев назад

Here are some videos to learn about acclimation process: www.youtube.com/@PlantCellTechnology/search?query=acclimation Hope you find them useful.

@stonheng 4 месяца назад

Im courious if they got contaminated or not

@PlantCellTechnology 4 месяца назад

We will update you soon!

@geoffgeoff143 15 часов назад

Tip. Do not use purple metho in a spirit lamp.

@nikkilynick9606 10 месяцев назад

Is there a difference between 70% and 90% alcohol

@PlantCellTechnology 10 месяцев назад

70% alcohol is more diluted having 30% of water, whereas the 90% is more concentrated with 10% of water.

@febeomnibeepboop6367 7 месяцев назад

the higher % decreases the time it sits unevaporated which reduces the time its killing bacteria, it also helps getting in to the cells to have some water in it. be aware tho as alcohol isnt all that good at killing mold spores, unlike bleach solutions

@antoniosanford4675 10 месяцев назад

The next step is for you to build a gene gun!

@febeomnibeepboop6367 7 месяцев назад

bathrooms are wet, wet is good for molds, molds are a contam risk. probably better not in the bathroom. kitchens have a lot of bacteria so also not the best. a good procedure with stil airbox etc however allows you to work in absolute filth :D the size of the space and thus draft matters a lot in this. watch out with stil air boxes SAB using open flames and cleaning products that are flammable ;), lol okay im still watching and kudos on that demonstration . i did see a procedure mistake here, when you use a S.A.B you want to organize it in such a way that you never have to reach over your work. i seen that hand hovering over the medium going for the burner, it would be better to set it in the center ( the burner also cleans the air btw by burning off any contam, bunsenburners are sometimes used to create a clean air bubble). this hovering over things can cause things to fall from your hands or arms. so always plan out the steps and set up things so you dont have to reach over things. work front to back instead of left to right. i always imagine the center being the place of work and the sides to be the hand moving area and the back corners are storage

@W4lkthroughWizard 2 месяца назад

Dont u have a window in your bathroom? Mine is dry af. You got to improvise if youre doing TC at home

@sweettooth5737 10 месяцев назад

I'm going to try growing some Japanese red maple seeds in TC this winter. Is the protocol different for starting seeds? I read that tree TC usually uses 1/2 MS amount but no mention of seed protocol. 🫤

@PlantCellTechnology 10 месяцев назад

Yes, generally protocols differ based on plant species and even the explant used in the process. We haven't tried the species yet in our lab. But, would love to get some update from you if you are going for it. Good luck!

@sweettooth5737 10 месяцев назад

@@PlantCellTechnology Thank you! I just got three cuttings from my neighbor. I know that I should take them in the spring but he wasn't sure he still be living there next spring. Will give updates. 👍💚

@PlantCellTechnology 10 месяцев назад

Amazing!! @@sweettooth5737

@PlantCellTechnology 10 месяцев назад

amazing!@@sweettooth5737