Drew, you are the best! I was a student of yours and I want to thank you so much for your amazing lectures and labs. They have made me so detail oriented that everyone at my workplace appreciates me for. I can never forget the pre labs for primary culture labs.You asked us to write every single step of this experiment which made this lab so more interesting and easier( other than cutting the the embryos head of course)Lol
Sir i want to perform some experiments on a muscle .... So i took out muscle from a chicken (not from embroy like after killing it) and i kept it in normal saline as a physiological solution and after adding a pinch of glucose ...will this setup of my will work and if it does how can i make muscles contract in vitro
This experiment is performed to make cell lines. You need fresh tissue that is not contaminated. Adult tissue does not work great, as the cells are bound tightly together and the cells are not in a state of constant cell division, like in the developing embryo. You will then need to disaggregate the tissue into individual cells, if you want to make a cell line. Without a circulatory system, the solid tissue will die as a result of a lack of oxygen and other nutrients. If you want to stimulate the muscle to contract, that is a completely different experiment. You need to harvest the muscle and find the nerve that stimulates it. Then you can apply an electrical current to activate the muscle fibers. I believe I performed an experiment like this on frogs back in third year Physiology. That was a course that changed my life.
You would have first of all egg punching with the help of egg punching machine then with the help of forcep first remove the punched egg shell,then remove the membrane and then with the help of thin pointed forcep remove the embryo it will be easily removed. Then put it in the petridish its head will be separated.
I have never heard of an Egg Punching Machine. How do you maintain sterility with this device? Removing the embryo directly with forceps can be a challenge, as the chorionic membrane and the yolk sac impede this. Finally, you state that the head will already be separated. What do you mean by this? I do not believe that any device can do this for you, as the embryo is floating inside the egg and be positioned in any orientation. What is your past experience with Primary Culture?
The majority of preclinical testing is performed on cell lines. To create a cell line students learn to extract tissue and disaggregate (break it down into individual cells). In addition, if you have a cancer diagnosis, you might have a biopsy taken to analysis the disease. Cell lines can be made from your cancer tissue and oncologists can test out different chemotherapeutic drugs on the cells in the lab. This way they can eliminate the drugs that will not work on your cells and they can focus on the best treatment for you before the cancer has a chance to spread. I hope this helps explain the importance of learning this technique.
I am currently doing a master's degree in the animal breeding department and I am also an agricultural engineer/zootechnician. And I am about to complete my master's degree by receiving a significant project scholarship in my country, which aims to manipulate the gender of chicks by injecting herbal and artificial materials with the in ovo injection method. I have provided long-term support to many projects using feed and animal materials on poultry. I am the first generation in my family to pursue higher education and I am looking for a PhD education with a scholarship. Due to my increasing interest in laboratory studies, I have also completed the laboratory veterinary health department and am in the internship phase. I am looking for an internship in a laboratory in the field of poultry nutrition or breeding for the summer term. I have a laboratory animals certificate and a B1 level English certificate. I followed some of your works on RU-vid with interest and reached you. I live in Turkey and I do not want to leave the field in which I work with interest due to financial difficulties. I would be grateful if you could guide me. Best regards
Sounds like you are passionate with what you do. That is so very important. Are you referring to the manipulation of the sex of the chicks? Gender is a social construct. Sounds like a very interesting project. What type of chemicals modify the sex of the chicks. They only gestate for 3 weeks. You must need to inject the eggs very early. How to you ensure sterility. I am unsure as to what kind of guidance you are looking for. I do not take PhD students, if that is what you are looking for. Happy to answer any more questions you might have.
