what a beautiful demonstration (and honest with all the mess haha!) Thanks for making this video Professor Drew, looking forward to many more cell culturing vids!
What method would you use for bone marrow isolation from the femur of that small chicken embrio ? The femur is too small to use a needle to extract the bone marrow. What method are used ? Thanks
Can you do a video of growing normal edible egg from scratch in the lab. Like ivf you take the egg from the hen, but you growing the egg in the lab without fertilizing it.
@@ProfessorDrewCollop I know, but I thought scientist now have a way to grow edible eggs that is not fertilized. Guess it's my imagination, I was just so interested in most of your videos. That's why asked if it's possible to have a edible egg in a lab without a hen. But since it's not possible, it's fine. Thank for responding back, I really appreciate.
There is a process to grow edible meat in the lab now, without killing animals. Search for "Clean Meat" or "Cultured Meat" to get more inform. A process to replace the creation of an edible egg is not cost effective. Hens are cheap and not worth the effort.
Hi Jose. There is a lab manual that contains these protocol; however, it is published by my College. I do not believe I can just send you a PDF version of it. I am very clear in the videos about the protocol and would be happy to answer any questions you might have, if you try to create your own protocol while watching the videos. Sorry I cannot be more help.
Hi there, wondering how long you leave the heart tissue in the fridge with the trypsin? And what are the steps that follow for this method? Having no luck with the transplant method and want to try this out.
Hi Hannah. Thanks for the question. In the past we had our students only leave them in cold trypsin overnight. They needed to come back the following day to complete Day 2. As Lab is scheduled with 3 day break in between labs, we tried them for 3 days in the fridge. Still worked to make a monolayer of cells after incubation at 37'C for 30 min. No problems. I believe the trick is cut the tissue up into very small chunks to increase the surface area. Let me know how it goes.
