I have passed my biochemistry exams thanks to your videos. It would be fantastic to have you cloned and placed in to our lecture halls :) Thank you for all your effort!
AH man, I was so stressed about the lecture before I watch your video. what my prof. try to explain in 50 minutes you did just in 10 minutes. very very thankful.
"AK LECTURES" are wonderful videos. These lectures are continuously helping us to understand the most complex processes of Biology and Biochemistry. We really appreciate this great professor of Biology/Biochemistry. And we are really grateful to him.
thank you 😊 tomorrow is my test and I was running out of time to prepare it but you explained the topic so well that I am quite confident for tomorrow:)
Hat off to you sir. I bet many people will find in these videos the enthusiasm and skill to teach that many times is kacking in their classrooms. Brilliant performance, :)
3:30 There is a mistake in the video on the picture there is not a DNA template strand but CODING strand because mRNA is made in 5'-3' end direction and all the names on the DNA strand are given as are on codding strand of DNA not template.
Super,thanks! A stupid question but how does enhancer sequence just bind to the site as a promoter? Does the DNA just bind to itself? How does it get there?
+AK LECTURES I think you should distribute this video into two, one for prokaryotes while another for eukaryotes. Thank you very much for your lecture! I really appreciated it! #bigfanfromyou
On the whiteboard is a diagram of 5’ to 3’ DNA with the pribnow promoter box shown to the right of the 5’ end of a DNA single strand. Yet you say it is the compliment of this strand that is read by the RNA polymerase starting at the 3’ end of the not shown “template” strand. If so, then what is the nucleotide sequence for our pribnow promoter site? Shouldn’t the nucleotide sequence of pribnow be the compliment of the shown TATAAT? Which is really ATATTA? Can you see the dilemma here?
I was watching your video on eukaryotic gene structure and there you said the RNA Pol II binds to the transcription start site. Now in this video you said it binds to the promoter. im confused....
Really cool video! But I'd like to mention, that when you describe CAAT box for eucariotic cells, it is written on the board, that it is GGNCAATCT, which can not be true, because there is no nucliotide N. According to wikipedia, it starts as GGCCAATCT (N is changed to C).
Sir i follow Biochemistry Book by Donald Voet and Judith Voet . I found lot many details in this book do you have videous that talks about these details ... I found your videous very helpfull .. Thanks for nice lectures.. :)
Great video! Thanks! Quick question. Is 35 and 25 distance from transcription in prokaryotes and 1000, 75 and 25 in eukaryotes hard distances, are they an exact average, or are they rough approximations?
They are only rough approximations because it might differs from different individual not every individual having the same amount of nucleotides in a certain strand of DNA hence when it was being transcript then the amount differs too. For approximations of -35 and -10 nucleotides upstream sequence are for prokaryotes while in eukaryotes are -25 to -35 nucleotides upstream sequence ( these are all just rough approximations ) nobody can give you the exact place for every individual as I mentioned it differs. :)
Says DNA template, however isn't the DNA template strand the complement of RNA transcript? It should be the coding strand, because it is not the strand that is read.
+Jonathan Weiss i guess so yes.. and its promoter sequence ranges from -25 and -75. and its somewhat obvious if there wont be any transcription process then how ur body would be able to produce proteins, as we know that RNA helps in protein synthesis. I hope m clear to you.
hello sir, actually i didn't get what do u mean by bacterial gene>? i mean i know what is gene but how transcription can start from -10 units or -35 units from bacterial gene? i just didn't get that point. :D @aklectures thanks for delivering worthy lectures!
+Misbah Ashraf - I think the answer here is that RNA polymerase spans a large region of DNA before the gene. The core enzyme has a mass of hundreds of kDa... So the complex is large enough, and has enough active sites, to recognise promoter sites. Transcription does not start at these sites. Transcription starts at 0. -10 and -35 are in relation to transcription start.
+Misbah Ashraf The promoter region is just where RNA Polymerase will bind to the template. It does not actually start the transcription process until it gets to the start codon nearby it.