This video explains about the Radical Scavenging Activity Measurement and IC50 Calculation from DPPH Assay in excel
DPPH Assay: Radical Scavenging Activity Assay - Principle, Procedure, Advantages and Limitations
• DPPH Assay: Radical Sc...
DPPH Assay:
DPPH assay was discovered by Goldschmidt and Renn in the 1920s. DPPH stands for 2, 2-diphenyl-1-picrylhydrazyl. It is a stable free radical with purple colour which turn yellow when scavenged. DPPH is used for measuring the Total Antioxidant Capacity (TAC).
Antioxidant capacity is an overall ability of organisms or food to catch free radicals and prevent their harmful effect.
The % radical scavenging activity of the sample can be calculated using the following formula,
%𝑹𝑺𝑨=(𝑨𝒃𝒔 𝒐𝒇 𝒄𝒐𝒏𝒕𝒓𝒐𝒍 −𝑨𝒃𝒔 𝒐𝒇 𝒔𝒂𝒎𝒑𝒍𝒆)/(𝑨𝒃𝒔 𝒐𝒇 𝒄𝒐𝒏𝒕𝒓𝒐𝒍)∗ 𝟏𝟎𝟎
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3 окт 2024
