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DPPH Assay: Radical Scavenging Activity Assay - Principle, Procedure, Advantages and Limitations 

Bio-Resource
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This video explains about DPPH Assay: Radical Scavenging Activity Assay - Principle, Procedure, Advantages and Limitations.
Calculation of Total Antioxidant Capacity [TAC] and the percentage Radical Scavenging Activity.
DPPH assay was discovered by Goldschmidt and Renn in the 1920s. DPPH stands for 2, 2-diphenyl-1-picrylhydrazyl.It is a stable free radical with purple colour which turn yellow when scavenged. DPPH is used for measuring the Total Antioxidant Capacity (TAC).Antioxidant capacity is an overall ability of organisms or food to catch free radicals and prevent their harmful effect.
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5 окт 2024

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Комментарии : 24   
@janettreezajoseph851
@janettreezajoseph851 7 месяцев назад
Sir, if the solution that gives yellow color gives higher absorbance value than the one with pale yellow, the activity % for the sample with brighter yellow shud have greater activity theoretically. But after substituting the values, the brighter yellow sample shows gives lesser activity value. Can you explain this?
@fyfauzie4029
@fyfauzie4029 2 года назад
What different between 1.1-diphenyl-1picrylhydrazyl than 2.2-diphenyl-1-picrihydrazyl?
@ceeblue392
@ceeblue392 2 года назад
Did you get?
@fyfauzie4029
@fyfauzie4029 2 года назад
@@ceeblue392 thats synonym in IUPAC
@416suchismitasutar5
@416suchismitasutar5 Год назад
What is the result of this method please mention it
@napoleonarmugam8391
@napoleonarmugam8391 2 года назад
good
@lakykhatun7119
@lakykhatun7119 2 года назад
How to prepare standard solution.plz and
@almche8978
@almche8978 6 месяцев назад
Yes please how can prepare it?
@grsbiosciences
@grsbiosciences 2 года назад
Please sir make video on superoxide dismutase and catalase
@amitpalai4654
@amitpalai4654 2 года назад
Why DMSO is used and can we take any other solvent.
@BioResource
@BioResource 2 года назад
Yes. Other solvents can be taken. But DMSO gives the best result. Ref article: dx.doi.org/10.13005/ojc/360316 Hope this helps.
@SanchariBhattacharyya
@SanchariBhattacharyya 6 месяцев назад
can you tell me what to use for autozero? on the spectrophotometer?
@yuu-chan6663
@yuu-chan6663 5 месяцев назад
Methanol or ethanol depends on what you chose to mix dpph in
@chandnipatel9236
@chandnipatel9236 2 года назад
How to calculate p value
@alexisbarnes1425
@alexisbarnes1425 10 дней назад
145 Rico Mission
@dpruthvikumar2689
@dpruthvikumar2689 2 года назад
DPPH is not cheap 😂man It is more costly than gold 😂
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