RECOMBINANT DNA TECHNOLOGY - AQA A LEVEL BIOLOGY + EXAM QUESTIONS RUN THROUGH

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In this video, I explain ALL of the content required for the "Recombinant DNA Technology" section for AQA A Level Biology. This includes: creating DNA fragment by reverse transcriptases, restriction endonucleases + the gene machine, amplifying DNA fragments by PCR + transformation using plasmids, marker genes and gene therapy. Time stamps are in the comments.
I also go through a few exam questions and explain their mark schemes.
The content in this video crosses over with other exam boards too.
Please leave a comment if you have any questions, I'll be more than happy to answer them!!! :)

Опубликовано:

5 сен 2020

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Комментарии : 34

@AlevelBiologyHelp 3 года назад

00:28 = What is recombinant DNA technology? 01:16 = Producing DNA fragments: reverse transcriptase 04:19 = Producing DNA fragments: restriction endonucleases 07:05 = Producing DNA fragments: the gene machine 08:58 = Amplifying DNA fragments: PCR 11:48 = Transformation using plasmids 14:51 = Marker genes 17:28 = Gene therapy 19:45 = Exam questions and mark schemes

@bogdanmocanu6600 Месяц назад

watching this 2 hours before my a level bio paper 2, thanks for your help.

@Theeliteworld3 Месяц назад

Same omg and I hate this topic 😢

@eaph-rr3re Месяц назад

here too 💀

@Emily-su2dh Месяц назад

Loll same

@unstablepanda1996 Год назад

Hi, do you need to know the advantages of PCR and the comparisons between Invitro an invivo, do you also need to know the advantages of isolating techniques like the use of reverse transcriptase? I have looked at the specification and it does not say specificly say you need to know the "advantages" or to compare them

@wombatcom Год назад

Can you explain what you meant when you said oligonucleotides are "overlapping" DNA sections? Thank you

@mattharris1930 3 года назад

wow, thank you so much! does a whole plasmid actual cross through the cell membrane (during transformation), is it not to big?

@AlevelBiologyHelp 3 года назад

Yes! Heat shock causes the membrane to become more permeable, so the plasmid can pass through ☺️.

@evajpaul205 3 года назад

thanks for the video! just have a quick question - why won't the recombinant plasmids survive in agar plates? i thought they have antibiotic resistant genes? its shown in your slide at 17:22

@AlevelBiologyHelp 3 года назад

Yes, but this is testing if the plasmids that have been taken up by bacteria are recombinant or not, not if the bacteria have taken up plasmids at all. A gene of interest is usually inserted into a marker gene (antibiotic resistance gene), disrupting the function of the antibiotic resistance gene, so if the plasmids are recombinant, the bacteria can't survive on the agar plate with antibiotic :)

@evajpaul205 3 года назад

@@AlevelBiologyHelp thank you so much, that makes sense! ☺️

@xlxmxh378 3 года назад

Hi, I really love your videos and find them useful so I was wondering if you do any other subjects, especially aqa a level chemistry?

@AlevelBiologyHelp 3 года назад

I don't at the minute, but I'm planning on making a Chemistry channel soon :)

@miinziiiiiii 3 года назад

If you’re looking for chemistry I’d recommend Allery Chemistry as they’re very specification specific and have separate videos on exam question practice too.

@RAHMANISHMIL 2 года назад

Get SNAPREVISE SERIOUSLY THEY BASICALLY TEACH YOU EVERYTHING AND PLUS YOU CAN ASK QUESTIONS AND YOU REVISION GUIDES LIKE FROM PMT

@relionabaq-harkenburg6306 2 года назад

@@RAHMANISHMIL no

@mattharris1930 3 года назад

how does using the GFP help u identify the recombinant plasmid when the flouresene only happens once the gfp is made, so this would be the same as identifying the bacteria that have taken up recom plasmids?

@bashredies8127 2 года назад

@@AlevelBiologyHelp would this not mean that bacterial cells that have taken up recombinant plasmids will be identifiable by the fact that they do not glow as the DNA fragment has been inserted into the GFP gene, so recombinant plasmids will have a disrupted GFP gene so wont glow

@AlevelBiologyHelp 2 года назад

@@bashredies8127 Yes, sorry for the mistake 😵‍💫

@barbod5096 Год назад

@@AlevelBiologyHelp bestie what does he mean im so lost lmao

@ShahbazPanezai 3 года назад

I feel good, I knew that I would, now

@miinziiiiiii 3 года назад

How is it converted from cDNA to double stranded DNA when cDNA is double stranded already?

@AlevelBiologyHelp 3 года назад

cDNA is originally only single-stranded since it's formed from RNA (which is single-stranded). The second stand is then formed by DNA polymerase. Hope this makes it more clear :)

@chifumnanyauzoma-awunor 4 месяца назад

@@AlevelBiologyHelp When you said reverse transcriptase can align the complementary DNA bases to mRNA bases, which DNA bases were you referring to? Thank you!

@rubiksworld2170 3 месяца назад

@@chifumnanyauzoma-awunorthere are free floating nucleotides just existing in the cytoplasm. Those are the ones used for dna replication to create a new strand as well!

@s.yusuf01 6 месяцев назад

at 23:53 i dont understand the answer you put in how does promotor DNA mean the sheep isn't going to be killed?

@AlevelBiologyHelp 6 месяцев назад

Because the DNA is obtained from milk so the sheep doesn't need to be killed to get the DNA :)

@miinziiiiiii 3 года назад

How does a calcium ion solution increase membrane permeability?

@AlevelBiologyHelp 3 года назад

Hi, as calcium ions are positively charged, they form an electrostatic bond with the phosphate groups of the phospholipid bilayer of the cell membrane, "neutralising" the charge. This means that the plasmid DNA is able to enter the cell (it wouldn't be able to do this without the calcium solution because DNA's negative charge would repel the charge on the phosphate groups). It's also a calcium chloride solution, may I add!! I hope this makes sense :)