Great video, my college biochem professor is making us watch this video for lab today! I'm glad you're getting exposure and views!! Great explanation and visuals.
@@animatedbiologywitharpan You made an SDS gel and you forgot to add SDS in your gel mix. You realized this after you loaded your protein on the gel and began running it. What will your results look like? How will the protein migrate in the gel in the absence of SDS?
Excelent video. But I think there is a small mistake when you talk about Glycine. Glycine pI is 6.06, so at pH=6.8 it will have a weak negative charge and not a positive charge. If it had a positive charge it would not run into the gel, but go the opposite way and exit from above.
Thanks for this comment, makes this video somewhat comprehendable... Cant believe I pay for a lecturer to direct me to videos like this that aren't even fully correct!!
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Such an informative and amazing video. Such visuals will definitely help the learners to grasp the topics easily. Much grateful! Hope to see such amazing content in the future as well. 😍
In order to make high quality content consistently, we need support from you. Please support us by using super thanks option. Super thanks icon is present below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Your small contribution means a lot for us.
Could you please say that how can we experimentally analyse whether a single band represents a single protein or two different proteins with same molecular weight.?
@@animatedbiologywitharpan hey thank you so much. Btw, apart from 2D , can we use western blotting rather with the help of antibodies specific to my desired protein?
