Hey, your videos amazing and very helpful, please can you make a video about how we do the analysis to the result after the pcr done by the thermocycler (biorad)...🙂
so what goes in the wells, how is the layout if we have standards and a reaction mix? Is it, reaction mix in all wells, and then in respective wells: cDNA of target genes, cDNA of housekeeping gene and cDNA of standards? i am so confused
What’s the point of keeping the pcr plate on ice? Theres nothing in the mm that degrades at RT and most enzymes have hot start mechanisms to inhibit activity at RT
You would be right most of the time. Some enzymes out there function at lower temps like the NEAR enzyme. Also, there are thermodynamics to all reactions and colder temps limit activity even if it's small. It's a general good practice so you can maximize sensitivity and consistency of your assay.
@@Filtrous interesting! I did not know about NEAR PCR and it sounds interesting. There’s really 0 activity in hot-start enzymes mixes at RT but I get what you are trying to say
