You are the man, Sir. The amount of work you must have put in to make all these videos is second to none. You are teaching (often times highly) complex matters in a way that makes it very easy to understand for your viewers, and you definitely contribute to a higher educational level among a lot of students, otherwise running their head against the wall, in badly written text books and badly executed lectures at university. Thanks a ton!!
I don't read my notes anymore. I juts watch your videos and I feel confident with upcoming exams. You're such a great mentor. From the Philippines here, Salamat :-)
You make the best biochem videos!! I watched your lectures for my biochem course and now studying for my MCAT! your explanations are x1000 clearer than text books and other resources. thank you so much!
I´m form germany, and I liked the explanation very much. Normaly it´s hard for me to follow english science videos, but you talk very clearly and explain the "stuff" often in differnet way, so that even I can understand these difficult processes. Thanks a lot!
I started med school years ago and then letf 1 year later and start over again this year 2022. Why am I saying this? Because now and then there are themes that its special difficult to me to understand and ALWAYS that I look for te solution on AK LECTURES I find it, but its not just a resolution, its a excellent and simple an clear explanation! I have no words enough to say thank you! You're just AMAZING! I wish one day to be as good teacher as you are! You're literally a inspiration for me! Thank you so much!
You take me back man! Been watching you since I first started at uni and now preparing for advanced upcoming exams you're still helping me and lots of students around the world, you're the best!
I really appreciate your work , such a complex topic I was able to understand after struggling over weeks in just 5 min after watching this in 2x, this is how I have written in my notes,. thanks
Your videos are really helpful with my molecular diagnostics class! Our teacher never explains things in detail and it makes it sooo hard to understand anything that she is saying! Thank you for making these! They're a life-saver lol
It was really awesome. you have taught such a hard topic with clear concepts. I have always feared from Genetics but It was very easy to understand. Thanks for this video😊😊😊
genius!!! thanks you speak very well, i´m a latin person and my english is normal jaja.. because the way you speak i could get every word you said!! bravo!
Hi there AK lectures, great video - you do a good job explaining the process. I'm wondering - what's the practical application of a southern blot versus a standard PCR amplification & gel electrophoresis genotyping method? Cheers! :)
The point is to isolate the sequence among a large numer of fragments. Maybe you have a sample of dna from a crime scene and you know just a small part of the killer dna, so southern blot can help you find that little fragment among the sample af dna you found in the scene.
Sir ur videos are really helpful, thank you for making them😊 but I would like to tell you one thing that some of ur videos have low voice , please correct it if possible.
Hey, great vid thank you ! I dont understand why we want to isolate the fragment in the first place if we already have the complementary strand !? Can someone help please :) ? i think im missing something
in step 3 after denaturing DNA, why does the denatured DNA attach to the radioactively labeled DNA and not the original complementary strand where it separated from?
I'm quite confused on what Southern blotting is used for. So just to repeat what you've said - In order for us to perform the southern blotting procedure we need to know the DNA sequence of our desired gene in advance (to make a complementary radio-labeled DNA strand). So two questions about that process: 1) So what exactly southern blotting is used for? Is it used for identifying if an organism has our gene or interest and that's it? What else could we possibly use it for other than that? 2) If indeed we use southern blotting for identifying if an organism has our gene or interest in its genome, why would we need to use the electrophoresis gel for? Can't we just denature the DNA strand and throw it with a radio-labeled probe to see if the DNA strand gets attached to the probe and then we can deduce the DNA strand does indeed have our gene of interest? Why would we need to cleave the DNA strand and run the restriction fragments through electrophoresis if all we search for is the gene of interest? Obviously I could be missing something but I'm quite confused on why exactly we need these additional steps as it seems redundant. Please help?
If anyone could answer that would be cool. How can we put complimentarty of strand of fragment of interest if we did not know which is our fragment of interest? And if we already know that? What's the point?
Regarding the DNA fragments that get transfer into the cellulose sheet, how does DNA denature? For my understanding the DNA in the gel is double-stranded? Maybe the the conditions during the transfer?
Hello sir. I have a query. Since northern bloating doesn't require denaturation (of ss-RNA), do we need to dip the gel inside alkali solution? Or is it necessary for knocking out certain Nitrogen bases for later radioactive labelling in membrane?
