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Spore to Agar update: Was it successful? 

Real Simple Mushrooms
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Come and join us as we provide you with the latest update on our spore germination plates! It has been a week since we inoculated 6 agar plates from the 3 most common spore collection methods: spore syringes, spore prints, and spore swabs. We inoculated two plates for each method, using a very simple potato agar recipe, which in our testing over the years has proven to be the most consistent at germinating spores of all types. If you are already a pro at agar making or even a beginner, we highly recommend giving our recipe a try it's below this description - trust us, it is incredibly straightforward and budget-friendly. Oh, and don't forget to hit that subscribe button so you don't miss out on any of our future mushroom-growing adventures!
Potato Agar Recipe: Makes 1000ml of agar
100-150g peeled potato (white/yellow preferred)
1500ML water
20g agar-agar powder
cheesecloth
2x 1 quart mason jars or laboratory glass
Boil the potatoes for 20 min, just a slow rolling boil....not raging.
Strain the potatoes, reserving the water. Run the potato water through a cheesecloth a few times to get any potato pieces out. Pour 500ml into each quart jar and mix in 10g of agar-agar in each until dissolved. Pressure cook at 15psi for 25 minutes.
Chapters:
0:00 - Intro
0:34 - Spore Syringe Plates
1:09 - Spore Print Plates
1:40 - Spore Swab Plates
1:49 - Bacterial Contamination
2:20 - Next Step in Process
3:15 - Conclusion

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30 июл 2024

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Комментарии : 11   
@neverwinterfarms
@neverwinterfarms 7 месяцев назад
Bouncing through all your videos. Really good breakdown and explanation of the task.
@RealSimpleMushrooms
@RealSimpleMushrooms 7 месяцев назад
I appreciate that!
@toddwmac
@toddwmac 7 месяцев назад
As said below, some great content. Kinda hard to see through the frosted plates. Was wondering if you could also describe a bit of what you are seeing when checking plates. For example, the "good" plates had some dark spots at the center of the colonies but I'm guessing those were just spore clumps. Learning to Identifying tam early on plates would be super helpful. Mush Appreciation!
@RealSimpleMushrooms
@RealSimpleMushrooms 7 месяцев назад
Yes, those are just visible spores. Bacteria looks completely different than mycelium, it's almost like a booger on the plate. Contams will also generally grow faster than mycelium sometimes to if you have something that looks a little on the fuzzy side and is moving and growing EXTREMELY fast it's more than likely contam
@paraglider1000
@paraglider1000 3 месяца назад
Thank you… i’m just beginner but you have changed my mind…I can’t wait for next one 😊
@RealSimpleMushrooms
@RealSimpleMushrooms 2 месяца назад
You’re welcome 😊
@D3ltaLabs
@D3ltaLabs 3 месяца назад
Looking forward to more of your videos. Great content as always. Ive watched all your videos atleast a few times each, some more.
@RealSimpleMushrooms
@RealSimpleMushrooms 3 месяца назад
I’ve been extremely busy with my full-time job, but I will have some new content very soon
@D3ltaLabs
@D3ltaLabs 3 месяца назад
@@RealSimpleMushrooms awesome, I understand completely irl is way more important. 😊
@evangelinedean
@evangelinedean 7 месяцев назад
Searching Amazon; do we need the “nutrient agar” or will the “vegan gelatin replacement agar” work? Thank you
@RealSimpleMushrooms
@RealSimpleMushrooms 7 месяцев назад
a.co/d/b25EgJ0
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