Тёмный
No video :(

Teaching a Beginner How to Make Plant Tissue Culture Media 

Plant Cell Technology
Подписаться 18 тыс.
Просмотров 2,4 тыс.
50% 1

In our "liquid" version of "Teaching a Beginner How to Make Liquid Plant Tissue Culture Media," your enthusiasm was clear - you wanted more! So, we're thrilled to introduce a series with Francisco and Shay that promises to be your go-to guide for all things tissue culture.
Today, we're diving into the foundational steps of creating the ideal liquid media for houseplants. Shay, with his expertise in fungi, is now venturing into the world of plants, and we’re documenting every step of his learning process. This first installment is all about crafting a nourishing base to kickstart healthy plant growth.
Here is that video, if you have not yet watched it: • Teaching a Beginner Ho...
If you want to get your hands on the Biocoupler™, here is the link: plantcelltechnology.com/produ...
🌿 In this Video, You Will Discover:
0:01 Intro
0:48 How to make plant tissue culture media
4:40 Calibrating the pH
6:46 Pouring the media into the vessels
8:51 Autoclave tips
9:15 Conclusion
10:14 Outro
🧤 Follow along as we measure, mix, and calibrate - all while sharing insights and best practices to ensure your tissue culture journey starts on the right foot.
📢 Join the conversation by subscribing to our channel. Whether you're a newbie or looking to refresh your skills, this series will provide a steady stream of accessible, entry-level content.
👩‍🎓 Plus, don't miss out on our Online Live Tissue Culture Master Class on May 11 and 12 - perfect for those ready to take their tissue culture knowledge to the next level. Register here: plantcelltechnology.com/master-classes/
Thanks for watching! Stay connected with us on social media for more educational content, and visit plantcelltechnology.com to get all the necessary products for your tissue culture endeavors. Use the code "FP10" for a 10% discount on your first order. Together, let's cultivate a greener future!
Don't miss out on all the fun and learning. Follow us on our social media channels for more tips, tricks, and plant science magic:
🌿 INSTAGRAM: / plantcelltech
🌿 FACEBOOK: / plantcelltechnology
🌿 LINKEDIN: / plant-cell-technology-...
🌿 TIKTOK: / plantcelltech
🌿 TWITTER: / plantcellnews
🛒 Products We Used in the Video:
- Supreme-Grade Agar: bit.ly/3T4nZy3
- MS Media w/ Vitamins: bit.ly/3YPAaAp
- PPM™: bit.ly/3ZLhXES
- Gellan Gum: bit.ly/421HWto
- IBA Solution: bit.ly/3ZysQKA
- BAP Solution: www.plantcelltechnology.com/b...
- Round Snap-Lock Containers: www.plantcelltechnology.com/r...
- Laminar Flow Hood: www.plantcelltechnology.com/2...
#planttissueculture #plantcelltechnology #tissueculture #micropropagation #plantscience #tissuecultureplants #tissueculturemedia #nepenthes #nepenthestissueculture

Опубликовано:

 

19 апр 2024

Поделиться:

Ссылка:

Скачать:

Готовим ссылку...

Добавить в:

Мой плейлист
Посмотреть позже
Комментарии : 31   
@aviationcompilation455
@aviationcompilation455 3 месяца назад
LOVE THE NEW SERIES. I WANT TO SEE A MUSA TC video!
@Alexander_Evans
@Alexander_Evans 3 месяца назад
So when you add the gellan gum to the deli containers, you don't have to mix it before autoclaving? Does it just mix evenly when boiling during the sterilizing process?
@PlantCellTechnology
@PlantCellTechnology 3 месяца назад
It would be best to mix it before and even after autoclaving (by swirling the media before purring it into culture vessels). This will ensure that the gelling agent is homogenously mixed into the media.
@zigzagdaPIMP
@zigzagdaPIMP 2 месяца назад
i also recently came to plant culture from fungi culture, and i as well never bothered with the PH for the fungi. But once i started learning more about PH i got curious about applying similar growing methods with fungi.. so far ive made too few experiments with too broad results,, so still hard to say about the need for controlled PH with fungi :)
@zigzagdaPIMP
@zigzagdaPIMP 2 месяца назад
great video by the way!
@PlantCellTechnology
@PlantCellTechnology 2 месяца назад
@@zigzagdaPIMP Thanks! It's great to hear about your experiences. Keep sharing your significant updates with us. We would love to know about them.
@theflaca
@theflaca Месяц назад
I'm all in. Been watching every video since it all began. Great to see Shay and fungi. I've never seen this cultivated. Best wishes to you all from Perth Australia
@PlantCellTechnology
@PlantCellTechnology Месяц назад
Thank you so much for all your love and support for our videos!
@ashleyjpahls
@ashleyjpahls 3 месяца назад
Is your class live? I’m out of town this weekend but would still love to take it.
@PlantCellTechnology
@PlantCellTechnology 3 месяца назад
Yes, we have an online class coming up that you can join: plantcelltechnology.com/pages/master-classes
@alfred282
@alfred282 3 месяца назад
Thanks for sharing.. Just wondering, can we keep the un-autoclaved liquid MS media (without agar) for more than 24 hours? When we are dealing with some different types of media, it will be easier if we have the MS liquid stocks ready beforehand
@PlantCellTechnology
@PlantCellTechnology 3 месяца назад
Generally, there are chances of contamination due to sugar, so we don't recommend that.
@davesharp8994
@davesharp8994 3 месяца назад
If it dosen't gell completely can you add more agar and re run in autoclave?
@PlantCellTechnology
@PlantCellTechnology 3 месяца назад
Adding more agar makes the media hard and does not allow the diffusion of nutrients into the tissues. You need to add just the right amount of agar and mix it well.
@davesharp8994
@davesharp8994 3 месяца назад
Thank you, so basically start over if media dosen't come out? I added 1/4 tsp to 83ml cups. I made 12 instead of 10 as that is how many I can put in autoclave. Thanks for your help
@PlantCellTechnology
@PlantCellTechnology 3 месяца назад
@@davesharp8994 You need 8 grams of agar for 1 liter of the media, to be exact.
@johnharding2912
@johnharding2912 2 месяца назад
Please please please do a video on embryo extraction/rescue for us. Would happily pay for private video as the litterature is very broad on this topic. Pretty please 🙏 😅
@PlantCellTechnology
@PlantCellTechnology 2 месяца назад
Absolutely! We will add it to our list.
@subhankarroy577
@subhankarroy577 2 месяца назад
Sir can you please do TC video on aquatic plant please 🙏
@PlantCellTechnology
@PlantCellTechnology 2 месяца назад
We do have some videos on our channel. Did you check them out?
@subhankarroy577
@subhankarroy577 2 месяца назад
@@PlantCellTechnology yes i did Sir. but i really need a specific video on aquatic plant as like as Montecarlo/ Ludwigia glandulosa/Anubias/rotala/Hygrophila🙏
@MDHTelevizion
@MDHTelevizion 3 месяца назад
What if were using dwk media
@PlantCellTechnology
@PlantCellTechnology 3 месяца назад
You need to be sure that the plant you want to culture has that requirement. It is better to follow a protocol.
@MDHTelevizion
@MDHTelevizion 3 месяца назад
@@PlantCellTechnology im trying to storage tc samples of my cannabis mother's
@PlantCellTechnology
@PlantCellTechnology 3 месяца назад
​@@MDHTelevizion You can try MS media supplemented with suitable hormones. That has worked in our class so far. Didn't try DKW.
@ozmalo472mtz7
@ozmalo472mtz7 2 месяца назад
Do yo have protocols in pdf or video for Aroids??? Such a great job, crongrats!!!
@PlantCellTechnology
@PlantCellTechnology 2 месяца назад
@@ozmalo472mtz7 Currently, we don't have any, but you can find a lot of literature online.
@subhankarroy577
@subhankarroy577 2 месяца назад
Please sir
@MrAlphaboubacar
@MrAlphaboubacar 3 месяца назад
Great Francisco repeating is pedagogy, but how to make media is have been visited too many times.
@mycrodexmushrooms
@mycrodexmushrooms 3 месяца назад
It's new to me! 😂
@PlantCellTechnology
@PlantCellTechnology 3 месяца назад
Many new members join the community regularly, so we need to revisit the basics to help them with that. :)
Далее
Smart Sigma Kid #funny #sigma #memes
00:26
Просмотров 12 млн
Tissue Culture Beginner's Guide - Learn ALL the Basics
10:53
Teaching a Beginner Aseptic Technique
24:14
Просмотров 1 тыс.
Can Tissue Culture Remove Hop Latent Viroid (HLVd)
4:48