Want to package recombinant lentiviruses? What packaging cell line should you use? How do you achieve higher titers? How do you harvest your lentiviruses after they’ve been packaged?
Lentiviruses are a key tool in today’s field of biology as they provide a reliable way to achieve stable over-expression of a gene in your cells of interest. However, many scientists are confused about how to produce these genetic engineering marvels in their own labs.
In this video, we'll outline the steps/protocol for packaging lentiviruses, including:
• Considerations before you start [1:02]
• Basic packaging protocol [01:33]
• Storing your lentiviruses [4:19]
• Infection test experiment [4:56]
• Additional tips and tricks for lentivirus production
After watching this video, you’ll be well on your way to becoming a lentivirus production champion!
[4:05] Note: An error in pronunciation. It is a 0.45 µm (micron) filter (not micromolar) that is recommended for use.
You can read more about how to package lentiviruses and get a hard copy of the protocol here:
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Background music credit: "Back To Portland" by TrackTribe
16 окт 2024
