The Sci Guys: Science at Home - SE2 - EP15: Extracting Strawberry DNA

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Welcome to the fifteenth episode of season 2 of The Sci Guys. In this episode we are extracting the DNA from a strawberry. We will show you how to mix a solution that will take apart the strawberry cells and release the DNA from inside them. Strawberries have a large amount of DNA compared to humans and we can easily extract it and pull it out of our solution with tweezers. Strawberry DNA looks like a cloudy ooze.
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Equipment and Ingredients:
Fresh Strawberries
Liquid Dish Soap
Salt
70% Rubbing Alcohol
Ziplock Bags
Mixing Bowl
Measuring Cups and Spoons
Large Glass or Beaker
Tweezers
Funnel
Strainer
Goggles
Gloves
Apron or Lab Coat
Previous Episode: Plasma Globes and Electric Fields - • The Sci Guys: Science ...
Next Episode: Newton's Disc - • The Sci Guys: Science ...
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Опубликовано:

10 июн 2024

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Комментарии : 330

@menace8672 4 года назад

anyone here from school

@mielnequinto5811 3 года назад

Void 0 not me

@yamete1729 3 года назад

Yes.

@nmasijika7557 3 года назад

⸻⸻⸻⸻⸻⸻⸻⸻⸻⸻⸻⸻⸻⸻⸻⸻⸻⸻⸻⸻⸻⸻⸻⸻⸻⸻⸻⸻⸻⸻⸻⸻⸻⸻⸻⸻⸻⸻⸻⸻⸻⸻⸻⸻⸻⸻ physiology

@jbauer21jb 3 года назад

Yup

@watermelon7215 3 года назад

Yeah

@tylerhyder-hobson2685 3 года назад

I typed out their method for people who need it for school: 1. Mix the water, salt and dish soap together in a bowl. 2. Place the rubbing alcohol into the freezer to chill. 3. Put 3 strawberries with the stems removed into a Ziplock bag, measure out 3 tablespoons of the previously made solution and pour that into the bag also. 4. Seal the Ziplock bag closed, remembering to remove the air and crush the strawberries until they are a fine, foamy paste. 5. Strain the strawberry pulp into the beaker, using a spoon to push it through. 6.Remove the rubbing alcohol from the freezer and measure out half a cup of it. 7. Gently pour your alcohol into the strawberry mixture, making sure that they don’t mix and that it just makes a fine layer on top of the pulp. This will help the DNA separate from the rest of the strawberry mixture. 8. Use your tweezers to remove the strands of DNA from your beaker.

@generalgusher9836 3 года назад

legend

@erinruffin9730 3 года назад

I can tell ur a cool guy

@samuelmusgrove7312 3 года назад

Absolute God

@Drexmss 3 года назад

I have 4 questions for school: 1. What do you think DNA looks like under a microscope? 2. Are strawberries the only fruit with DNA? 3. What does DNA look like in the experiment? 4. Where was the DNA in the strawberry?

@strkeys5923 3 года назад

Your a legend thanks

@VentMagic102 8 лет назад

Can I perform this experiment on my children? I don't have strawberries.

@TheSciGuys 8 лет назад

VentMagic102 preferably not ?

@VentMagic102 8 лет назад

The Sci Guys ...oh. okay. i'll be sure to avoid that minor mistake next time.

@the_weeb_lord1017 7 лет назад

The Sci Guys Can I eat the strawberry DNA and become the worlds first strawberry human hybrid

@antoniocheairs1810 7 лет назад

XboxMLGProGamer make sure its not male gametes

@E.GodLike 7 лет назад

XboxMLGProGamer do so

@ArtisticallySpoken 4 года назад

"Place your rubbing alcohol in the freezer to chill" Should it have sunglasses when I take it out the freezer?

@eliaspartain 3 года назад

sí

@anxietyatitsfinest286 3 года назад

absolutely

@squidward9600 3 года назад

did anyone else science teacher assign this to them

@yara-dj6ps 3 года назад

yep

@Cy1ord 3 года назад

Same

@roboman4729 7 лет назад

I once tried this on myself by using my saliva instead of the mashed strawberries, and it actually worked!

@UltraHanisah 6 лет назад

How much saliva did you use? Probably up to a cup to make your DNA really visible, haha. I only get a dot of DNA from about 2mL of saliva

@shaynnelove7919 5 лет назад

do it on your blood!!!!!

@kneeger1948 5 лет назад

200 iq madlab boys

@your3171 4 года назад

I can’t tell if y’all serious or not 😭💀

@ariadnajalifegomez1567 3 года назад

@@your3171 Saaaaaame

@sylveraltima 3 года назад

shoutout to Mrs Schroeder's class

@LA4Tara 5 лет назад

I really appreciate this! So simple and neat! Also I really liked the animated explanation as well, helped a lot for visualizing the process, thanks! Subscribed!

@JacksonNorum 5 месяцев назад

AGREEEDDDD!

@billyboybliss 2 года назад

As a Science teacher, this is one of the more helpful videos for my university students, especially the animation. Keep up the great work.

@krislawson3569 7 лет назад

great diagrams and explanation of the reactions - thanks

@toastandtea347 2 года назад

Thanks for the reasoning part too. Nice lab presentation.

@albatra0z_ovaries282 7 лет назад

I did this experiment at school last year under standard curriculum, and now we're redoing it under honors; I've yet to see the changes implemented for this year in contrast last year, but last year's was pretty similar to this experiment. I don't think our teacher trusted us the tweezers ...

@Bathtub_Jim 7 лет назад

Thanks! Bill Nye does this on his new Netflix show but doesn't go into the details. I appreciate that you did.

@shaynnelove7919 5 лет назад

so, this helped a lot with my research paper for science class thanks!!!!!!!!!

@TheSciGuys 9 лет назад

Hey Justin, I couldn't reply to your comment directly, probably because your comment is linked. You can do this method with a lot of different fruits. The thing to remember is that if the fruit isn't octoploid you will require much higher quantities of it to get a significant amount of DNA. You might be able to spread the DNA out on a slide and look at it under a strong microscope. You may not see much, but you might see something.

@WiIIis 4 года назад

Hey Sci people! I'm doing a science report on this lab exercise and since we're in distance learning I'm using your video as the lab exercise. I was just wondering if you were able to tell me why it is necessary to chill the rubbing alcohol before adding it to the strawberry mixture. if you see this please reply!

@smilejianyi 7 лет назад

Hi Science Guys. Something to ask on. Is the extraction of this experiment can be further use in genome sequence reading? Is the DNA clean from impurity?

@shackledore 8 лет назад

Hey Guys! Great video but is only DNA extracted or is other cellular material also collected here like proteins?

@mr.johnzussino6217 2 года назад

Great video - thanks.

@justinmcgillivary3702 9 лет назад

Very cool. Will try this out. Is there anything that can be done with the DNA besides just looking at it? Can this method also be used for other fruits or plants?

@gabriellestalker1857 9 лет назад

I am doing this in the lab today. I liked how you explained the chemistry to us! Thanks for sharing!

@tsunamisquid300 3 года назад

Instructions unclear, I drank the dish soap.

@SynoAuRi 8 лет назад

I used 91% alcohol,instead of 70 and it STILL WORKED!!! THANKS AND I WAS SO HAPPY WHEN I SAW IT!!!

@TheSciGuys 8 лет назад

+Cool Swagman Awesome news!!

@ryanmackay35 4 года назад

you madlad

@mianajdovski5845 2 года назад

does anyone know how the greater amount of dna in a strawberry affects the way it functions? for instance, a raspberry has less dna and i’m trying to figure out the way it affects it as a whole.

@guzmy8068 8 лет назад

tanks i need to see this to make an inform about this LIKE!!

@AlejandraPerez-wz1zq 8 лет назад

Would it be an advantage or disadvantage that the strawberry DNA is protected by a two membrane system in the cell?

@Yousefmmohammed 3 года назад

Thank u that is so helpful and fun to watch😇💙💙💙💙💙💙💙💙💙💙

@mariumthekiya7646 5 месяцев назад

thank you

@anitaratnasari1492 5 лет назад

Thanks for this video.. from Indonesia..

@seijuroakashi5084 6 лет назад

How would I know if this is really the DNA? Because I don't have any tools to see if it is a real DNA. Thank you!

@vv-ky4bi 5 лет назад

What we can do with this dna??? Can we use in any metal...any synthesis of metals using this??

@petergoettler1385 5 лет назад

SCIENCE is FUN...Thxs.

@jimmythegreekone Год назад

Are there other extension activities that can be done after extracting the DNA?

@1babyisaac 3 года назад

What substance was used to get the DNA outside of the cell membrane?

@johnburns1351 9 лет назад

To make it easier for those trying to make a filter. A coffee filter will do as long as your strawberries are squished very thoroughly. H.S. Biology Teacher. Great video!

@TheSciGuys 9 лет назад

John Burns great idea :)

@shraddhapatil5940 6 лет назад

Like strawberry Is blueberry an octoploid??

@kiralft 7 лет назад

Hey please help, can I use ethyl alcohol instead of isopropyl alcohol??

@lady_ebao 4 года назад

Is there a certain brand of soap that I should use or is any dish soap applicable?

@Stefescope 9 лет назад

And the layers are like the layers of the earth! Denser material on the bottom! Cool! :D

@TheSciGuys 9 лет назад

Bubblegumpop396 If you haven't seen out liquid density tower experiment yet then you should!! You would probably like it, we layer 12 different liquids on top of each other.

@Stefescope 9 лет назад

I haven't seen that one yet, but i will definitely check it out!

@hiinsanity 4 года назад

What do you do after? Like how do you read it and test it?

@DoctorPickle 6 лет назад

Good video

@paulvusi4023 5 лет назад

chemistry experinments such as titrations

@haadiyahamjad7188 7 лет назад

+The Sci Guys I have a very very important question and i need to know the answer very quickly! Okay so i decided i will be doing this experiment for my science fair but when i tried it the cloudy thing is not forming. I dont know why. plz help me! Reply asap. and also how long did u wait till the cloud thing formed? plz reply quickly. i need this experiment

@samuelmusgrove7312 3 года назад

Did you end up doing the experiment anyway?? 😬😂

@saulbaeza4185 9 лет назад

I had a problem with making the layer of alcohol. It made it, but it formed beneath the strawberry mixture. Do you guys know why? (I did pour it gently)

@TheSciGuys 9 лет назад

Saul Baeza Hmm. I am not sure why that would have happened. Did you accidentally swirl or mix it?

@Yousefmmohammed 3 года назад

Does anybody knows where the organelles of the strawberries go after rupture of the cell wall by squishing it ????

@daniniai 7 лет назад

In my practice, I put the salt first to smash the strawberrys and then the detergent, actually, i used pineapple juice and i don't know why and i don't find any information of that :'v The result was a little bit of DNA. Could somebody explain that? (notice my terrible english)

@shadi.y 6 лет назад

can we use this flavor for e juice?

@jadencavan7247 8 лет назад

My class did the experiment without salt, but it still turned out good. My science teach said that my group extracted the most DNA than most he saw.

@TheSciGuys 8 лет назад

+Jaden Cavan That is awesome!! Congrats.

@nichelle2378 3 года назад

Can I use a mortar and pestle instead of a plastic bag?

@JacksonNorum 5 месяцев назад

AWESOME SAUCE!!!

@deeptisunil2756 7 лет назад

Can u specify what alcohol should be used....I need a quick reply pls

@TheSciGuys 7 лет назад

+deepti sunil rubbing alchohol. We found it at a pharmacy.

@mikkidoonan8696 7 лет назад

Silly question- but would this method work with other materials? Food wise, but also granulated substances such as sand, dust, crushed rock etc. I have to perform this experiment to prove that the provided samples that are not necessarily 'alive, may contain living organisms...not sure if this will work.

@anxietyatitsfinest286 3 года назад

There won't be enough living organisms to produce visible amounts of DNA

@andrianapatrick1343 5 лет назад

Does the alcohol have be to be cold ?

@ashley8659 Месяц назад

How long does it take for the dna to show ?

@secretzrevealed 5 лет назад

Do we need a funnel?

@coffeyjo9988 6 лет назад

That was awesome. Ive isolated animal DNA in my genetics lab and it was significantly more complicated!

@vv-ky4bi 5 лет назад

Do u know how to isolate virus from animals??

@user-bd2cf7kr4h 4 года назад

@@vv-ky4bi LiTtLe DiD yOu KnOw...

@ryanmonaghan436 2 года назад

@@user-bd2cf7kr4h SDKFJGFD NAUR

@amikkororo 3 года назад

That laugh tho- 'Mwah hah hah' Swapfell!Sans is that you?-

@Cy1ord 3 года назад

Lol

@samuelmusgrove7312 3 года назад

Does anyone know if this works without cooling the rubbing alcohol before hand?

@mianajdovski5845 2 года назад

yea i did this without freezing it beforehand and it worked

@panzerdegree9984 8 лет назад

Hey dudes, for how long should I put the alcohol in the freezer

@med8615 7 лет назад

it just has to be cold.

@TheJoewachsjr 6 лет назад

Hello, I'm teaching overseas. So i have to rely on locals to buy my supplies. They got ethyl alcohol instead of isopropyl alcohol? Will this still work or should I send them back out for iso? Thanks

@BIOL1306 6 лет назад

Ethyl alcohol will work fine.

@shaynnelove7919 5 лет назад

yes, it will

@thegamegeekcosplayandmore5159 5 лет назад

I know that I'm like 5 years late but I was wondering if sea salt would work

@your3171 4 года назад

I know I’m like a year late but no 😵😪

@semaankaram67 4 года назад

why put the alcohol in the fridge?

@sammie3641 3 года назад

The alcohol needs to be cold because it helps increase the yield of DNA. Cells contain enzymes in the cytoplasm that help destroy viruses but protect the DNA. Hot water has the potential to damage DNA by accelerating enzymes that break it apart. DNA is typically protected from such enzymes by the nuclear membrane, but the soap added previously destroys it. Cold alcohol helps the DNA precipitate faster.

@mattacquaviva2196 3 года назад

What is the purpose of salt in this experiment?

@RuskiVixen 4 года назад

I was always under the impression one can see a DNA only under an electronic microscope, is it not the case?

@ylcbffl1999 7 лет назад

Is there anyway i can store this ? My science fair project is next week and I would like to make this today ,how should i store this ?

@jamesrobinson949 7 лет назад

Yesenia Lopez Just do it twice! it's good to do labs more than once, usually each time you complete a lab you get a little better at it!

@tone-qd1ng 4 года назад

I realize your science fair was two years ago... anyway... since I'm quarantined, why not reply. Just pour it in a small container with alcohol.

@BusinessGenius1 5 лет назад

I Want To know. can I eat the DNA? What will happened if I eat daily. Will I get younger

@anxietyatitsfinest286 3 года назад

That DNA was in dish soap, I don't think it's a very good idea to eat it

@rajeshjagtiani1055 8 лет назад

Is it compulsory to use rubbing alcohol

@TheSciGuys 8 лет назад

+Rajesh Jagtiani yup

@Drexmss 3 года назад

@kelli7329 8 лет назад

What would happen if you did drink the solution made in the experiment?

@TheSciGuys 8 лет назад

+leila jade You would get very very sick

@richfiles 8 лет назад

You can use a high proof alcohol (ethanol) and fresh pineapple juice to do this as well. Skip the salt and soap, and instead freeze the strawberries and then let them thaw. Ice crystals will break up the cell membranes (much like the soap). Put about a cup of fresh pineapple juice (not pasteurized) in a baggie with about three strawberries, and squish. Strain the strawberries, and then pour the adult beverage the same way that the rubbing alcohol was done here. The DNA will separate, AND the entire concoction is now made of 100% human consumable ingredients. The Pineapple juice replaces the salt, and the freezing of the strawberries eliminates the need for soap.

@nataliaderylo 7 лет назад

this video was made when i most needed it

@nataliaderylo 7 лет назад

never mind, wrong year XD lol

@cyber4370 4 года назад

Nice

@petermedina7024 6 лет назад

Cool man

@C1418OS 4 года назад

Can I send this to 23andMe and get results?

@karan.kk.h 3 года назад

What would happen if we combine that dna with human dna

@forevertheeprincess 6 лет назад

Sooo cooolllllllllllllllllll!

@Chelssums 9 лет назад

What do you do with the DNA? I did the lab last year with peas. But we didn't do anything with the DNA! What can we do?

@WolfieSkye 9 лет назад

Chelssums You can keep it, or observe it under a microscope. Put it in a test tube filled with the remaining alcohol and keep it, or you can let it dry out.

@TheSciGuys 9 лет назад

Chelssums Wolfie Skye I agree with Wilfie with what you can do with the dna.

@mielnequinto5811 3 года назад

What if i put it in a seed

@princess_elviana_21 Год назад

Is the DNA you extracted pure?

@CrazyChicken306 8 лет назад

Yay

@sorsorscience0787 8 лет назад

can i use methanol instead of rubbing alcohol

@TheSciGuys 8 лет назад

+Sor Sor Science07 not to my knowledge

@sorsorscience0787 8 лет назад

The Sci Guys ok thank oyu

@misyagi 7 лет назад

I did this at school

@shafirachiesyaulayya4847 7 лет назад

so hows the result? bc me and my friends going to presentation this at school

@jong8271 6 лет назад

no one cares

@BerserkBird 8 лет назад

about how much dna is extracted per strawberry?

@TheSciGuys 8 лет назад

+BerserkBird Not sure on an exact volume or weight. It depends on the size of the strawberry as well.

@morgannelson8784 6 лет назад

does anyone know why the Isoprophyl Alcohol needs to be in the freezer????

@sammie3641 3 года назад

@marynichols9857 3 года назад

hi you guys are cool

@snabbott 9 лет назад

Gee, Brain, what do you want to do tonight? The same thing we do every night, Pinky: try to take over the world with strawberry DNA.

@jasonlast7091 6 лет назад

Can someone please tell me how to site this as a source with Harvard referencing? My paper is due tonight at 11:59pm

@N0TZEN 4 года назад

I'm so sorry no one responded...🙄 Hope your paper went well.

@fitswho 4 года назад

lol

@iteachpsychology 5 лет назад

hi sci guys, check out my class' activity based on this experiment: ru-vid.com/video/%D0%B2%D0%B8%D0%B4%D0%B5%D0%BE-LVyxOEbFKok.html we also did the pineapple enzymes breaking the gelatin experiment. i'll upload it the soonest the kids finished their videos.

@T4r4ng0 7 лет назад

can I use any fruit like bananas. need quick reply

@fahdafaisal1717 6 лет назад

yes

@afroacademy6254 7 лет назад

hey sci guy can u use the same method for men

@joplinjohnson3042 3 года назад

You said I couldn't drink it but can I eat it

@CoachFlores23 7 лет назад

Austin Evans brother?

@nickcalabrese7312 2 года назад

That was absolutely not three tablespoons of the salt-soap solution

@aidanlucas9110 5 лет назад

This has been the Children's Fun Fact Science Corner (Comment if you're a Night Vale fan)

@jackd5181 6 лет назад

Is it possible to genetically combine that with another fruit similar to that? ( other berries)

@jaa5097 7 лет назад

Извлечение ДНК из клубники. Ингредиенты: соль, жидкое мыло для мытья посуды, вода, спирт. Мыло разрушает клеточную мембрану (барьер), которая состоит из липида (жира), - там в ядре находится ДНК. Соль помогает выпасть в осадок все ненужное - оболочки клеток, ДНК будет в верхних слоях раствора. В воду добавляют соль и жидкое мыло, этот раствор выливают в полиэтиленовый пакет с клубникой и давят ее. Через 10 мин. процеживают раствор. В сок клубники добавляют охлаждённый спирт. При добавлении спирта происходит отделение ДНК и затвердевает как полимер (как сопли). Затем вам нужен будет сканирующий электронный микроскоп (Scanning electron microscope), чтобы рассматривать ДНК.