Trigonelline Increases NAD, Improves Muscle Function, And Extends Lifespan: Vincenzo Sorrentino, PhD 

Timestamps: 0:00 Introduction 1:25 Sarcopenia definition 3:45 NAD's links with health-related outcomes 5:05 NAD, trigonelline are low in sarcopenic patients 8:44 Trigonelline properties 10:35 Trigonelline is more stable than NMN, NR 12:26 Trigonelline increases NAD in human muscle cells, C. elegans, and in mice 14:40 Trigonelline is bioavailable in multiple tissues 16:40 Trigonelline increases NAD through the Preiss-Handler pathway 19:36 Trigonelline restores mitochondrial function during NAD depletion 22:05 Trigonelline benefits muscle morphology and fitness, and increases lifespan in C. elegans 23:50 Trigonelline improved muscle function in aged mice 27:157 Trigonelline is associated with muscle strength in people 28:37 Q&A 31:00 Trigonelline dose to increase NAD in people? 33:12 Trigonelline is produced by the gut microbiome? 35:25 Where does trigonelline's methyl group go upon conversion to nicotinic acid? 39:52 Trigonelline is increased in chronic kidney disease, which links with sarcopenia-would that suggest that trigonelline's doesn't positively impact muscle? 43:15 Trigonelline in centenarians: longevity marker or poor kidney function? 44:20 Does Vincenzo add trigonelline into his diet? Some sporadic video quality issues after 35:00, apologies for that!

More NAD increasers. There are a lot of them. Still waiting to see if high NAD in the elderly leads to higher lifespan.

Norway study (2018, Midttun, et al) showed plasma trig of about 3.6 micromol/L for 2 to 4 cups of coffee per day. zero cups per day was 0.3 micromol/L in plasma. 1 cup per day 1.6 micromol/L.

Great presentation and content from your guest

Great discussion - thanks. Keep us updated! :)

This is awesome content. Thanks for putting this together.

Great questions at the end! Very useful. Thanks!

It sounds very exciting! Perhaps it could be also beneficial for patients with chronic post viral conditions like ME/CFS and Long Covid

Thanks again.

thank you very much, that was very exciting!! On question left for me: the stability of trigo in plasma is quite high, and at the same time the NAD levels rise. Could we conclude that it is not directly converted into NAD via Preiss Handler, but rather has an upregulating effect on NAPRT? The stability is probably very important to reach all tissues, not just the blood compartment. Regarding plant sources I found an interesting paper: Hammel e al, 2013 ."N,N-Dimethylpiperidinium (Mepiquat) Part 1. Formation in model systems and relevance to roasted food products" ...which is not directly about Trigo . Yet they measured the Trig content of various seeds, and found that Trifolium incarnatum, Crimson Clover seeds contain even more Trigo than green coffee beans. Moreover, the whole plant is edible, and it is known that plants synthesize Trig for seeds and young tissue. ..and it is very cheap: 25kg cost 3$

Hi Michael, Have you found any data on methyl-niacin vs niacin? It's not really available to buy, and the little that is - very expensive! (Less that 3X of the effect vs niacin doesn't justify at the moment from a practical perspective IMO. )

That's very good stuff right here, I'm going to dig on this subject for sure! By the way, I know you made a video on the methyl pool in the body, do you recall which on was it please? I can't find it /:

Man I enjoy hummus with red pepper. Looks like I have an excuse to eat more hummus and drink more coffee ;) thanks

chatbot says beetroot got considerable amounts of trigonelline as well 🤔

"Trigonelline increases lifespan in C. elegans." I read a study that rosmarinic acid increased lifespan in C. elegans the most. The study title is "Plant and fungal products that extend lifespan in What are your thoughts on rosmarinic acid? I drink rosemary tea. It tastes very good with stevia.

Been alternating between niacinamide (NAM) and niacin (NA)

So ... eat lots of legumes and vegetables. Basically what was implied in the fiber video a few days ago. Good to know!

on amazon there is green unrocasted decaffeinated coffee beans available how much trigo do they contain tho? without messing with one's caffeine intake, considering trigo is solvable in alcohol, one could toss those beans into a wine bottle and just wait until one had trigo-wine this trigo wine then could be used as a (pre-meal) apéritif/digestive bitter to increase bile acid flow, its like a win-win-win combination of things

Im getting some made

Trigonelline for the win! I would have liked if they compared it to NA besides NR, since NA is cheaper and readily available.

Thanks Michael. Appreciate these presentations/discussions with scientists reviewing recent work. I have 1 cup of coffee plus 1 cup of decaf coffee in the morning before exercise and walk. Wonder whether there is benefit to exercising with a transient increase in NAD+ , beyond that provided by exercise, assuming my coffee increases NAD+ (although data shown here seems to show coffee, via caffeine marker, does not increase trig).

If methyl-niacin is 3x more effective, bro science tells me to triple my flushing niacin NA dosage to match methyl-niacin. Sticking with that until further notice. And take TMG to protect my methyl groups

OT: Suggestion for a topic to explore - the relationship between seasons, RHR & HRV, and mortality. Does RHR increase / HRV decrease in winter just because of temperature? Or is there something else involved, like sun exposure or geomagnetic flux? Are people better off in terms of health and longevity by moving to a warm, sunny climate (typically lower latitude) in retirement - or do they just need to stay active? I'm sure this topic would be of interest to many people. Personally, I've noticed a reduction in chronic pain when I can get out in the sun - I can feel it almost right away. In the summer I was able to do 20 reps of 25 lb Romanian deadlifts - now I can only do 6 reps and stop because of hip pain.

I started taking fenugreek seeds to get trigonellin.

Did he say that decaffeinated coffee also contains the same amount of trigger Nelly?

I drink coffee, and its trigonelline properties seem promising, however i feel like the undiscussed issue with coffee is the fact is excretes sodium, which in turn causes u to need more sodium --> and then consume more sodium. i LOVE coffee but i am slightly skeptic😖

What about green coffee bean extract as a source of trigonelline? Would that be an option? Any way to guesstimate the trigonelline content? What was a reasonable(guesstimated) dosage for humans? I had a hard time understanding the audio.

Can't find any reasonable way to get this in my diet. Highest source is Japanese radish which is only available in Japan. Second highest is coffee, but I refuse to add any amount of caffine to my body even in decaf amounts of 2mg. Fenugreek seeds in high amounts would not be good news, as the interviewee suggests they may have adverse effects.

I use a 1/4 theespoon fenugreek in my soups ..... but the problem is that my smell is going strange and my pee is smelling strange. I was advised 10 years ago to take fenugreek by a Chinese Traditional Acupuncturist .

i wonder how much trigonelline is in (unroasted) green coffee extract on average 🤔

Is trimethylglycine TMG with nicotinic acid combo the same as methyl-niacin?

Exercise, coffee and ketosis are cheapest options.

What do you think about the Nature article published today - 'A terminal metabolite of niacin promotes vascular inflammation and contributes to cardiovascular disease risk'? Unfortunately, it's paywalled so I can only read the abstract.

I can't understand the audio.

PS Vincenzo said (@ 46 min) that 3 to 4 cups of coffee increase plasma trigonelline to about 8 to 10 micromolar and was likely not enough to significantly impact NAD+. My Iollo level was 1.8 micromolar, much less than 8, yet I am a coffee drinker though not right before blood draw. So now I'm wondering whether foods can affect NAD+ via trigonelline, and if not why not. Is this just a freak thing where you need very high levels of trigonelline, outside of dietary ranges, to move NAD+?

to be continued...

what are the richest source of trigonelline pls?

OK, Vincenzo Sorrentino, let's go into business together and provide Trigonelline to the people as a supplement. How much would this cost as a startup?

Drink coffee. Haha that coffee breath will do wonders for your oral microbiome 😉.

Get a new mic and camera. Sound like you are in a tunnel.