Western Blot (WB) Visual Protocol 

This... this is an amazingly beautiful blot. Hard, black bars, no background noise, no unnecessary ladders. I think I'll just print it out and hang it on the whiteboard. You have my respect.

Thank you for the tutorial. It was very helpful and informative and has given me a better understanding of the protocol. I will be running my first ever Western Blot in the next few days!!

THANK YOU!! Very clear, concise and articulate.

This is a great video. Very informative to collaborate with my presentation on western blotting. Thanks!!!!

Absolutely brilliant and very descriptive...thanks a million

Outstanding. From someone who tested hiv negative using eclia and just found out if positive it is recommended to use this technique. I am stunned, amazing work

Very detailed clear exhaustive you have taken away all my doubts . Spectacular step by step. Amazing

Really thank you. This video is really easy to understand. Thank you again.

Thank you so much for uploading this video 😊🙏

Simply amazing..!! Brilliant

A very beautiful blot.

Thanks for posting this. Helps me with my exam! :)

Thank you so much. It is very clear and helpful WB video for me

Very well explained, helped me understand the protocol much better !! Thanks for the video !!

awesome. now im ready for my experiment next week

Great, thank you!

Very helpful tutorial video, thanks

thank you, it was helpful

Thank you. So amazing!!

Thank you 😊

the video was very helpful and explained each steps very clearly.Thanks a lot.

Very well explained

very well explained.... thanks a lot....

it was perfect i really appreciate

Thank you!! Page bookmarked.

We want more biological techniques vedioes in this manner.. please upload more videos of other techniques in detail 🎉😊🙏

This is cool

amazzzzzzzing

Great tutorial! I like the two chamber tray used in phase 3, membrane transfer. where did u get it from?

GOOD

when we incubate with more then 1 Antibody do we have to block for 1 hour before every incubation

Great video! Amazingly helpful and very well explained. One little question: In this case, what was the final ECL volume used on the last incubation? I know that it depends on the surface of the membrane (e.g. 0,1mL ECL/cm^2 aprox.) In my case I have a 7 X 8.5 cm membrane, so I just need 6 mL of ECL (following the manufacturer's equation). The problem is that I'm not very sure if 6 mL would cover the membrane in that type of Incubating box that you use (mine is extremely similar). What would you do in my case? You would increase the volume of ECL even is not needed or you would incubate just with 6 mL ECL. Thank you again for uploading this great video. It help us out a lot!

Premade gel!? I have to make mine.

when and where do you put copper stain?

Bicocca gang

How i manage to do all that at full concentration working inside a lab which is monitored the whole time with a obnoxious lab lady?

hmm why is she putting her hand outside the hood and then back again without cleaning them ? if everything must be sterile, you should never stick your hand outside the hood.