The MIQE guidelines published in Clinical Chemistry in 2009 clarify this issue: RT-PCR is reverse transcription PCR. Real-time quantitative PCR is qPCR.
Being new to PCR and stuff, I was really finding it confused , especially after going through the literature. But here? It's a wow, never I had come across of such an explanation which not just cleared my confusions but paved a way for some novel research ideas. Ton of thanks. Warm appreciations and looking for more of this kind. Thanks again. Good day.
I'm suspecting this will be one of those channels that will be incredibly successful in years to come. Growth will be slow especially on RU-vid, but if you continue producing incredibly informative and quality explanations like this, I'm sure you will go far. You explained this concept perfectly. Thank you!
Thank you so much! You are the best explainer ever. Plus, you have such great energy and demeanor, you are also fun and pleasant to watch and to listen to. I hope you are a teacher!
the RT PCR Test used for Covid-19 - Though it is easy to trace the discovery of Reverse transcriptases to Howard Temin , and its independently isolation by David Baltimore in 1970, and it is also very easy to identify the inventor of underpinning PCR Test as Kary Mullis, I have yet to identify a scientist or group of them that take the credit for each of the RT PCR Test (real Time vs. Reverse Transcription). Do you know? Does anyone know?
You are not showing on your graph the number of copies. Like you said ten versus 10000 is not shown on the graph. I get the guicker plateau in fewer cycles and that of course means more copies for that sample, but it's not plotted on the y axis.
So can you use primers for specific genes that code specific proteins and then use RT PCR in both its meanings to quantify how much there is of each mrna?
If there is PCR product after reverse-transcription PCR, then there was SARS-CoV-2 in the original sample. If there is no PCR product, then there was no SARS-CoV-2 in the original sample.
Incredibly clear, engaging, and informative video! I needed a quick refresher and I now am totally comfortable with the concept of RT-PCR and qPCR. Thank you for making this!
Sorry if I sound incorrect here, but through explaining Reverse Transcription PCR you explain how when you have regular DNA at the cDNA stage, does that imply you have left the Uracil in it, with not using RNAse H and Extending the DNA from the fragments to create a double stranded copy DNA of the original mRNA?
Helloo, so we know that DNA polymerase cannot use RNA as a template to build a complementary strand. What is the biological reason therefore? Is it due to structure or direction of activity?
Thank you so much for this!! I needed to understand how exactly real time pcr worked for a case study I have to present in front of pathologists for school.
@@ahmadhegazy7811 I can't recommend specific books in my field - we tend to learn techniques through direct mentorship from other grad students or postdocs in the lab. However, if you're looking for step-by-step video tutorials, definitely check out Jove www.jove.com/
