Hi Peter, thanks for asking. In average, 21,000 cells were in the 70 mL cell suspension (= 3 x 10^5 cells/mL). Please find the link to the Instructions here: ibidi.com/img/cms/products/labware/open_removable/E_8XXXX_CI_2Well/IN_8XXXX_CultureInsert_2Well.pdf A detailed protocol for a wound healing assay using MCF7 cells can be found here: ibidi.com/img/cms/support/AN/AN21_Wound_Healing_Assay.pdf Hope this helps!
Hi Setia, thanks for your question. How to quantify these assays can be found in our Application Note AN21: ibidi.com/img/cms/support/AN/AN21_Wound_Healing_Assay.pdf
