Yeast 2 Hybrid (Y2H) system: protein protein interaction technique

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What is Y2H, its requirements and how it works? An easy and animated video. Y2H is a molecular genetics technique to validate protein-protein interaction through downstream activation of a reporter gene. Yeast two-hybrid is based on reconstitution of a transcription factor (TF) fragments fused with two proteins whose interaction needs to be studied.
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by - Abhishek Bhandawat

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17 сен 2024

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Комментарии : 46

@sarthakgupta4915 2 года назад

best explaination of Y2H ...well written definitions and easy to understand

@XploreBio 2 года назад

@Sarthak. Thanks

@diyaroz3068 4 года назад

Thanks for saving time due to your very simple explanation. Keep it going!

@XploreBio 4 года назад

thanks..

@sadiasheikh9362 2 года назад

This is the best explanation of Y2H. Not a single soul on this Earth can explain it this well. Hat's off !

@XploreBio 2 года назад

Thanks a lot.

@mrinalinisharma7483 5 лет назад

best way to explain ; BIG THANK YOU

@XploreBio 5 лет назад

Thanks your comments acts as motivation to make more such videos..

@vishghare 5 месяцев назад

Very nice explanation 🎉

@anumohan5230 3 года назад

Informative, clear my doubt.Thankyou sir

@komalkhedkar6687 2 года назад

there is a mistake in this explanation you said the selective media lacking trp and leucine and plate containing trp and leu. but in diagram you have written selective media lacking trp nd leu. and plate lacking trp and leu. please correct it very confusive

@nepaliandhimalayasmusic1809 5 лет назад

It is really great video, awesome

@juliantorresflores371 4 года назад

Good explanation, !Thank you!

@pateltejasvig.2622 Год назад

Great i understand it very well. Thanks for your efforts 😊

@yasintopcu4042 4 года назад

Very well documented and easy to grasp even small details! Thanx!

@XploreBio 4 года назад

thanks..

@imtiazahmad5631 4 года назад

Awesome 🧡

@shivanikhemka 5 лет назад

Very nicely explained.

@shivanikhemka 5 лет назад

Where do you get the information from? Is it from a paper, a book? Please let me know as my research project is on this and it would be very helpful.

@XploreBio 5 лет назад

My videos usually are based on multiple resources. For this video I read some research articles and books and covered basic concept. Based on my imagination gave it animated. If you are a research student, I recommend you to refer related research articles.

@medielijah 4 года назад

very clear and concise video! namaste

@XploreBio 3 года назад

Glad you enjoyed it!

@weightelk 3 года назад

Very clear. Thank you!

@peerzadazubairahmad464 3 года назад

Very very clear. Thanks.

@ameliac7338 2 года назад

love this! Thanks

@manishakalsain6799 4 года назад

Thanks for the explanation

@XploreBio 4 года назад

Stay tuned. Thanks

@sandeshtelang1608 3 года назад

Very helpful

@ashley-janeduplessis4197 4 года назад

You are a lifesaver

@XploreBio 4 года назад

Thanks Ashley

@agentgunnso 2 года назад

Thank you is very ilustrative! I get it :D

@amnakhalid123 3 года назад

Thanku

@sundarapuraju892 5 лет назад

Unique way of teaching thanks sir

@alpaslanaksoy533 4 года назад

How do we make sure that some other transcription factor (instead of our plasmid inserted bait & prey) can't turn on the transcription of the reporter gene?

@XploreBio 4 года назад

Thats an excellent question. TF binds to specific UAS sequence and not any UAS, hence highly specific in expression of reporter gene. Also, negative controls are always used.

@lebohangmoshanyana1573 2 месяца назад

you could counter-select using the Reverse Yeast 2 Hybrid Screen. Look it up

@alpaslanaksoy533 4 года назад

Namaskar :)

@sumairamajeed9763 4 года назад

Gud

@michaelak6753 4 года назад

Why can both proteins not be on the same plasmid?

@avijitmalakar3059 3 года назад

If we place both of the proteins in one plasmid, we also need to place the AD and DBD in the same plasmid. That means the domains (AD and DBD) are already in close proximity, leading to the recruitment of transcription machinery and ultimately results in the expression of the reporter genes. We can,t study the Protein-Protein interaction by placing both the proteins in one plasmid.