The sample extract as well diffusion, and the positive control or standard as disc diffusion in the same plate. Can we differ like this sir?? Just curious to know 🤔
Sir, I want to know, when one is using a plant extract, unlike conventional antibiotics that have a standard, how can one determine the concentration of the plant extract that inhibits the bacteria when using MIC
U can add 2-3 drops of melted MHA to the well (purpose is just to seal the lower part of the well. But if the extract is viscous no need to seal the well. Extract u can add up to 100μl.
Thank you for the video! May ask in what book can I find this step-by-step process? Just so I can put a reference book for an activity for school. Thank you!
Sir in comments ,you said that if the extract is viscous ,we dont need to add melted MH agar at bottom, in your experiment also the extract is viscous then why you added MH agar on bottom?
I dont think the size of the well matters in the case. Its what you add to the well that gives you the clearance zone. so the more powerful the antimicrobial activity of the solution/extract, the bigger the zone of clearance and vice versa.
Dear Sir, would you please tell me about how to prepare the sample? In my case, when I filtered my sample through 0.2 micron filter inside a laminar flow cabinet, my sample's concentration as well as the volume also get decreased. So, I am now in confusion about how to prepare the sample correctly. Please help!!
Dear sir How to measure the ZOI in agar well diffusion method. since incase of Disc Diffusion assay the ZOI is measured end to end which also includes the area under the disc. in this case there is a well and couldnt be counted as clearing zone
Hello my products are neem extract nutmeg extract combo of neem & nutmeg extract herbal gel & marketed preparation. My need extract is viscous with what solvent I should dilute with?? Tween 80?? Can't I add above products directly?????
Thank you for the video sir. What control disc have you used in this video sir? I have a similar experiment with curcumin nanoparticles, but they are in paste form, not liquid form, do you have any suggestion in how I should load my sample inside the wells?
When u do this procedure first u add full con: of ur extract in to the well and check the zone of inhibition and compare the zone with antibiotic zone (commonly used antibiotic used for the bacteria u inoculated). If the zone is big then u can perform the MIC/ dilution and again repeat the procedure to get a good result with less dilution.
Before adding the extract, seal the underside of the well. When adding the extract, ensure not to exceed 100 microL. After incubation, if no clear zone is observed or if bacterial growth surrounds the well, it indicates that the extract concentration is insufficient........
hello sir, i am working on fungus but I am unable to culture it because of contamination, and how else can I try to culture and isolate fungus from seed cake surface.please help by suggesting something
U can transfer the fungus to the media by loop. When fungus grows, u can see different colours (means different type of fungus). With a sterile loop Touch only on to the top (spores) of one type of fungus (touch only 1 color) and transfer to the media and incubate the plates in an inverted position.
Well diffusion technique helps to check whether ur extract is having good antimicrobial activity or not. When u get good zone of inhibition after this technique, U can perform broth dilution method with ur extract for MIC. www.slideshare.net/RejoJacobJoseph/antibiotic-sensitivity-test-by-dilution-method
