Thank you for making such useful video, if it's possible then pls add text steps by step for better understanding. And one more thing if you have sop of this procedure then pls let me know 🙏
دكتور ..هل ممكن الافادة رسالتي مستخلصات نباتيه ونفحصلهم antibacterial activity لكن في تناقض بين نتائجي ونتاىج المختبر بسبب عدم ايجاد مذيب مناسب للمستخلصات
Dear sir, please help! I am conducting the antibacterial assay of my plant sample following the well diffusion method. But I have faced with a problem. Should I filter my plant sample with the help of 0.2 micron filter inside laminar flow cabinet before adding the samples into the wells. I found that when I filtered my sample, the concentration as well as the volume of my prepared sample also get decreased. I have also gone through severe literatures of my field but found no paper mentioning the use of 0.2 micron filter. So is it necessary to do so or I can skip this step?
You can try filtering through 0.65 u membrane filter. And if you have enough amount of the extract you can autoclave part of the extract. This will sterilize and checks heat stability