Plant Extraction Methods - Decoction and Maceration | JPTV 

That was so helpful man! Thank u doc. Stay immensely blessed ✨

Wow this is 3yrs video and it's helpful to me this year thank you so much Doc.

impressive and practically well-teaching video. thanks

This is a very comprehensive guide

Nice content lalo na for students. Very helpful 👏🏻👏🏻👏🏻👏🏻

Thanks doc. Learned a lot

big help gyud ni sa ako specially sa school.. thank you kuya John Paul ☺️☺️☺️

Great video!

Thank you for sharing your knowledge po ☺

wow this is a great video thanks for this!!!

Thank you so much from Brazil

This is very informative! Support ito, grabe!

Thank you, sir! This has helped me in understanding better the procedures I need to do for my thesis.💚

Love it so much 💖

your video is super helpful. thank you so much

great work

Thank you brotherJohn Paul

it helped me alot!

Thanks doc!

Excellent

Thank u man💚💚

Thank you for sharing bro.....

Good job

2:43 please.. have a question.. what type of solvent you have used for maceration ? I have another question : for ex if we use ethanol-water as solvent, after the extraction we eleminate the ethanol first using ratary-evaporator,but how we eliminate water fraction ? Thanks brother for sharing this video !!

Hi, Sir Doc, I find your video very helpful in our on going project study, I just want to ask if we use boiling of the roots, only through centrifuge is applicable to get the final extract or we can use the rotovap? Thank you Sir! I also subscribed in your youtube channel.

OMG thank you.

Thank you so much for uploading this video. ALLAH BLESS YOU ❤️❤️❤️

in oil extraction,how would you compare maceration and cold pressed using a machine,which do you think is more potent?

Do you have published paper that i can cite you on this method? By the way, thank you for sharing this! Very much appreciated.

thank you

I'm a 4th year pharmacy student and want to be involved with pharmaceutical science research. I've been thinking about taking a Masters in Pharmacy, but I'm second guessing because from what I see the type of research produced is usually assays of plant extracts which I kinda don't like. The type of research I want to do is along the lines of polymers, nanotech, etc. (i haven't quite figured it out yet). Do you have any advice for me?

great work sir , can you help me with a way to extract tannin from a plant ?

I would like to ask if there are other ways of extracting epicuticular wax as water resistant coating from leaves? We're just going to use it for our capstone project. Thank you!

hi Doc, do you have a related study about decoction method that we can use as a citation? we are currently working on a study but we are lack of citations

Hehe! Kawa ka mo ng mga video about make shift lab equipment since hindi naman lahat may boiler at vacuum ek ek. Para sa mga mahilig mag experiment pero wala pambili ng equipment. Hehe

nice...

hello! good am I am a 4th-year high school student I just wanna ask what is the best solvent to use for maceration for extracting the components in the plant and also good for the skin?

Thnq for such a valuable video sir..... Sir can u pls resolve my quiery...if we want to prepare a plant extract (for any activity) for whom we dnt have any idea about its solubility then which solvent will we use?

was hoping u would make the hplc ms/ms portion as well (:

pls do a video proceeding to HPLC analysis :)

Hi sir. Are there any alternatives for rotary evaporator? Can you use simple heating to remove the solvent?

Geez Louise, and we all wonder why our skincare or medicinal extracts costs so much; well....Exhibit A, ladies and gentlemen!! I need to remember this AMAZING process, shown to us by the kind Doctor. the next time I'm shopping for extracts and whining that they "just cost too much" at $14.95 a bottle; am I right? Thank you, Doc, for opening my eyes and giving me some perspective!

Sir I want to add natural vitamin E in my syrup formulation as my research work from vit e rich fruit or vegetable...so how to extract ?plz rply

Hello sir I have ..I've kept coriander leaves for maceration (for antibacterial property against p.acne) .but only using water I did not use ethanol or methanol with the water ..is it possible to obtain the concentrated extract from it and if so wt should be the temperature maintained in the water bath and also the pressure

one thing i want to ask, can you please share the procedure of solvent extraction for plant bark extract, for its phytochemical analysis.

hi sir! i'm currently in jhs and we're planning to extract wax from leaves. do u think this method is applicable?

Hi doc! What did you use po for filtration? Which is better po to use for filtration, cartridge of UF membrane or qualitative filter paper?

Very nice sir... I have a question? Someone who don't have fuming hood then how can be evaporated the remaining solvent? As by keeping in open air it takes lot of time to evaporate... ur reply will be appreciated sir...

Sir Please suggest food wastes( plant or vegetables) for the extraction of polyphenols for my dissertation

Hello doc! Pls answer this because I really need it for my team's defense How many extracts will be if I have 3kg of bougainvillea bracts using the maceration method?

Hi. What HPLC machine will be good enough to read Thc content pritty exakt?

Thanks doc. What's the ratio of the extract to solvent?

Estoy trabajando con extractos para mi tesis y no logro eliminar el etanol del agua, me puede explicar a que temperatura esta el refrigerante y cual es el vacio que utiliza.

Man I like this laboratory. Thanks for the video. This is the third time I am watching this. So after extraction how do you fortify your product with the extract?

What type of solvent are you using?

How to chose best solvent for Phthalates extraction? GC-MS analysis

Hello sir. Good pm. I am a 4th year college conducting a thesis. I have few questions regarding in our study. we use Flower extracts to develop an alternative highlighter pen inks. now I want to know how to get the concentrations of the inks? I hope you can help me sir. thank you in advance 🙂

Hi Sir, how many plant powder and solvent during maceration in this video? Thankyou

Im interested in plant extraction and recrystalization can you do a video for that

Good day, I would like to ask if what plant extraction is applicable to acquire its epicuticular wax from it?

Thank you for sharing but I need more clarifications how can you help me?

hi ! how much of saluyot did you use in this procedure?

Which paper is used for filtration?

I wanna ask how much the ratio of plant sample and solvent do you use. If i use 5g of dried plant how much of ethanol do i need to dissolve the sample?

Good day! The plant powder that you used in the beginning of the video, was it store-bought or did you prepare the powder extract? Thank you!

Hi sir, may I ask how many grams are in the vial bottle after rotary evaporation. Thank you

so the vapour temperature is basically the solvent's boiling/evaporation point?

I have a stupid question but please help, i want to extract nutrients from Seaweed to use it as fertilizer does boiling water denature or destroy any useful amino acids or plant hormones like auxins or cytokinin etc?

Hey really nice video, so i wanted to extract anthocyanins from Jamun fruit using maceration with ethanol, do I need to dry the fruit or can I just simply put the fruit inside the solvent ?

I always heard extractions where done with alcohol, butane, propane, and co2 Infusions where done with water, oil, viniger or a low proof alcohol like wine ?

Hi doc, can you make content about extracting polyglutamic acid from soybean and turning into a powder?

What is the shelf life of the "extracts" when performing extraction process? In my case, I consider Iyphilization/freeze drying the plant to achieve the same texture of green tea before boiling it. I wanted to store a large amount of dried leaves for future purposes and save it for decoction. Will it affect the shelf life?

what solvent did you use po for the extraction? i am working on my research paper similar with this methodology however i don’t have enough knowledge about it since i am only shs student and i don’t have consultant as well bcs of this pandemic.

Hi. This is really informative, can I ask what are the exact volume / measurement of the used materials here? What is the ratio of the plant to solvent? Will there be a difference in the result if the amount of extracted plants are bigger? Thank you.

Doc how many grams of plant powder was used for macerating?

Is this method of wet extraction of inulin from sisal bole???

Hey doc how r u doing that was a great video. Can you please show us fraction distillation of essential oil to extract different components from it or any other technique. For example I want to seperate citrol and Other components

How to extract any mineral from the plant? Thanks.

Him Thanks for this very informative video. Can decoction be used for stem bark?

How'd you identify what ratio of solute:solvent to use?

Hi Doc! We are currently working on a research where are going to extract Peroxidase enzyme from papaya to detect glucose in a urine through GOD-POD method, but we are struggling in finding a laboratory that will help us to extract the Peroxidase from Papaya so that we can have a analytical grade peroxidase that we can use for our research. Do you have laboratories that you can refer so that we can contact them?

Hi! your video helped me so much. But can I ask a few questions to clarify? 1. What is the best solvent to use for decoction method for extracting phenolic content? 2. What is the best solvent to use for decoction method for extracting flavonoid content? Your response would be very much appreciated because I need this for my research methodology. Thanks!!

Hai, when you freeze dry. Is it ok to fill almost full or leave a small space in your centrifuge tube? Because the technician in the lab that I'm using freeze dryer would tell to fill just half. but I filled the same as in your video. Thanks.

May I know how can I determine the chemical properties of a certain plant?

Can i ask for list of all lab materials you used in the process?

This video is very insightful. May i ask if you don’t mind, when using the decoction method what is the reasoning behind freezing? And if necessary how will it be possible to obtain a liquid form of the extract instead of the powder? Thank you for any response and for this video.

How to extract Bisphenol A (phytoremediated) from plants... Plz help

Awesome video shall I know the best method for extracting Ginseng herb? can we use the same method? please advise and thanking in advance

I have a question, the powder that i have used for the maceration can i still reuse it? After filtering my solution, there was still leftover solute. After some time, it became dry again. And so i was hoping if i could use it again to make another batch of the maceration process. My plant material is very limited, you see. I used methanol as my solvent btw

hello doc, your video is a big help to me, May I ask, what is the recommended ratio of solid to solvent when performing maceration. thank you so much

Hello Doc, I would like to ask if it is possible that after decoction, I filter the mixture through a whatman filter paper and freeze dry the liquid extract without the use of a centrifuge and rotary evaporator? (I am using an aqueous extract)

Good day Doc. Please which is a better polar solvent between aqueous and ethanol in extracting plants bioactive compounds against microbes?

Hi . Can you tell me the equipments name ?

please help me to answer this question: What strategies can be employed so that the extraction and isolation of natural products are done under mildest possible conditions?

your rot' evap' setup ... a few questions : 1. what is that thing the sample flask attaches to ? it looks like some sort of splash - guard and does it have a part number ? 2. the condenser glass thing ... does it have a part number too ? you did well with the camera work to capture the p/n's for the rotavapor and heat - bath. with all these p/n's one will be able to shop for them

what solvent did you use in maceration of the plant? :)

Hello! I am currently doing my research in plants. I have extracted it with ethanol extract and already airdried it. However, it contains chlorophyll and I am trying to remove it using a separating funnel with non-polar solvents. But there is a lot of confusion, can you explain how can I remove chlorophyll without destroying my active constituents in ethanolic extract also which non polar solvet is best or if there is any other methods.

Good day, Doc. Doc, how long did you store your crude extracts in your fume hood for further evaporation? I mean how long does it take to make the crude extract from syrupsy to dried form, if possible? Thank you in advance, Doc.

hello sir! What if we don't have rotary evaporator. Can u suggest some alternative for the extraction?

Hello po! Thank you po for the informative video! It really helped us understand the methodology needed to conduct our research. May we ask po how much plant material (in grams) do we need to use to extract 5mg, 16.25mg, 35mg of the pure extract? And also how much solvent is required for maceration? We are currently struggling to compute the amount of grams of plant material to get the according milligrams (We tried using the vague references in the video but still struggled). Also po we need to compute the amount of solvent used for maceration and the ratio is 1:2 (w/v). The volume of our final solution is 50ml and we need to dilute it for 3 concentrations (10%, 32.5%, 70%). After solving this, the grams needed for the dilution is already stated above (5mg, 16.25mg, 35mg). To conclude, our group wants to know how much grams of plant material is needed for the final product using the 1:2 ratio for maceration and how much solvent is required. **We know that this request is a bit hard, if it cannot be answered, do you have any suggestions po on how we can compute it?** Thanks po doc!!