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This page will give you a good idea about transformation trouble shooting www.thermofisher.com/in/en/home/life-science/cloning/cloning-learning-center/invitrogen-school-of-molecular-biology/molecular-cloning/transformation/bacterial-transformation-troubleshooting-guide.html?open=transformants#transformants
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Let's say 10 plasmids are there in the solution so ideally 10 should go inside ( assuming transformation efficiency is 100% ) . But in reality it's near 60-70% so 7 would make it and 3 would not . In reality we use plasmids in nanomol concentration. Now you can actually calculate the number of plasmid used for transformation.
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Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.
Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.
