I use the RNA Pico Kit but I´m wondering about your gel preparation. In my instructions it says to centrifuge the gel (spin filter) and add the dye AFTER (centrifuge again).
When you said you are adding gel/dye mix to other 3 gel wells but you already added 1 out of 3 gel well the gel/dye mixture for priming. Do you have to add gel/dye mixture again on that well?
Thank you very much for this video. It helps a lot! However, may I check on the reagents (especially all related to the dye) because as what I read in the Agilent High Sensitivity DNA Kit Guide, I understand that the reagents are light-sensitive. But in this video, it does not seem that way. Can anyone help in this matter? Thank you in advance.
Hi Lilian, I use this instrument daily for my job in cancer research and in our lab we always store the reagents in a dark box due to it being light sensitive especially the dye that gets mixed with the gel as well as the other reagents.
Hello everyone, how are you? I wondered how much it would be necessary to pay for a small lab capable of doing NGS? Can anyone help me? Thanks in advance! :)
