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Biochemical Tests for Bacterial Identification (Part-1)_TSI, MIU, MR-VP, Lysin, Urea, Citrate 

MicroChem's Experiments
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Biochemical tests are very conventional & popular method for the detection and confirmation of bacteria after isolation from any sample. This video presents a detailed procedure of several common Biochemical Tests (TSI, MIU, MR-VP, Lysin, Urea, Citrate) those are needed to confirm, specially, a gram negative bacteria according to ISO 6579-1:2017.
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#Biochemicaltest
#Identificationofbacteria
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#Gramnegativebacteria
#Detectionofgramnegativebacteria
#Salmonella
#Salmonellatyphi
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28 июн 2024

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Комментарии : 121   
@navaneeth_us
@navaneeth_us 2 года назад
Beautifully crafted and articulated video. Thank you so much.
@MicroChemsExperiments
@MicroChemsExperiments 2 года назад
You are welcome. Stay with us.
@tayyabaramzan2343
@tayyabaramzan2343 2 года назад
thank you....this video helps me alot in my open-ended lab completion
@MicroChemsExperiments
@MicroChemsExperiments 2 года назад
Thank you
@sridevikannan188
@sridevikannan188 2 года назад
Ur explanation is really a great one and very useful for my research work....thank you so much...
@MicroChemsExperiments
@MicroChemsExperiments 2 года назад
You are most welcome
@snehalaxmichoudhury2084
@snehalaxmichoudhury2084 2 года назад
Nicely described... easy to understand. Thank you
@MicroChemsExperiments
@MicroChemsExperiments 2 года назад
You are most welcome
@safaafrin5391
@safaafrin5391 7 месяцев назад
Great❤ clear explanation
@sonandpets1215
@sonandpets1215 Год назад
You are awesome!! Thank you for sharing!
@MicroChemsExperiments
@MicroChemsExperiments Год назад
Thanks for being with us.
@learnmedicallabtechnology2152
@learnmedicallabtechnology2152 2 года назад
Masha Allah Superb Explanation. Thank you 🌹
@MicroChemsExperiments
@MicroChemsExperiments 2 года назад
Thank you
@rambabu7652
@rambabu7652 Год назад
Excellent and clear definitions.. good effort
@MicroChemsExperiments
@MicroChemsExperiments Год назад
Glad you liked it
@redvelbeth314
@redvelbeth314 Год назад
Very interesting, I’m gonna have to practice soon cos we have a upcoming practical exam about this
@MicroChemsExperiments
@MicroChemsExperiments Год назад
Best of luck!
@redvelbeth314
@redvelbeth314 Год назад
This is very helpful thank youuu
@MicroChemsExperiments
@MicroChemsExperiments Год назад
You're so welcome!
@tubaidatta9662
@tubaidatta9662 Год назад
Well done.
@MicroChemsExperiments
@MicroChemsExperiments Год назад
Thanks
@user-ve2op9dh8u
@user-ve2op9dh8u 2 года назад
Thank you I have understood 😍
@MicroChemsExperiments
@MicroChemsExperiments 2 года назад
You are most welcome
@shereenomer3391
@shereenomer3391 Год назад
Very good 👍
@MicroChemsExperiments
@MicroChemsExperiments Год назад
Thank you 👍
@ajithazhageson7260
@ajithazhageson7260 Год назад
GOOD CLARIFICATION
@MicroChemsExperiments
@MicroChemsExperiments Год назад
Thank you
@waqasmaqsoodkahloon1798
@waqasmaqsoodkahloon1798 2 года назад
Very nice
@MicroChemsExperiments
@MicroChemsExperiments 2 года назад
Thanks
@user-bu1ki1sm5b
@user-bu1ki1sm5b Месяц назад
and also i have methyl red indicator directly how do i use it?
@vinayomar4974
@vinayomar4974 3 года назад
Would you please prepare a video teaching how to calculate Measurements Uncertainty for any test. For eg. Moisture / FAT / pH etc.
@MicroChemsExperiments
@MicroChemsExperiments 3 года назад
Ok we will. Stay with us.
@user-bu1ki1sm5b
@user-bu1ki1sm5b Месяц назад
i dont have innoculation needle can i do this using Loop?
@charliefasurf5098
@charliefasurf5098 Год назад
In the step three: pure colture - 01, need to inoculate the colonies from XLD to TSA plate. Which XLD plates need to choose? Those previously obtained from selective enrichment of RVS broth or MKTTn broth, or both? Thank you for your videos and for your work.
@MicroChemsExperiments
@MicroChemsExperiments Год назад
Subculture to TSA from XLD is important. You should choose colonies all XLD plates (from MKTTN and RVS, both)
@charliefasurf5098
@charliefasurf5098 Год назад
@@MicroChemsExperimentsI would have another question. The ISO does not specify the non-selective medium to be used, can alternative media to the TSA be used? For example plate count agar or yeast extract agar?
@MicroChemsExperiments
@MicroChemsExperiments Год назад
@@charliefasurf5098 You can use plate count agar
@charliefasurf5098
@charliefasurf5098 Год назад
@@MicroChemsExperiments To confirm the presence of salmonella, all biochemical tests must be positive (in particular TSI, Urea and Lysin). Is it possible the tests give a positive result for colonies obtained from RVS selective enrichment and a negative result for colonies obtained from MKTTn selective enrichment (or the reverse)? In this case what would the outcome be?
@user-kq7ri6zd9t
@user-kq7ri6zd9t 2 года назад
Great video presentation!, May i ask why is there a need to loosen the caps of the tube before incubating it?
@MicroChemsExperiments
@MicroChemsExperiments 2 года назад
To facilitate oxygen transport into the tube (to make the aerobic condition).
@user-kq7ri6zd9t
@user-kq7ri6zd9t 2 года назад
Thank you!
@MicroChemsExperiments
@MicroChemsExperiments 2 года назад
@@user-kq7ri6zd9t You are welcome
@mohdabrar2839
@mohdabrar2839 3 года назад
thanks
@MicroChemsExperiments
@MicroChemsExperiments 3 года назад
You are welcome. Stay with us.
@islaserena1599
@islaserena1599 2 года назад
Hello, can I know what is the volume of media are needed in broth tube for the culture before biochemical test is performed?
@MicroChemsExperiments
@MicroChemsExperiments 2 года назад
It varies on type of media. Some are 5 ml per tube, others are 10 ml for each tube
@livegames2533
@livegames2533 2 года назад
Thsnks,, so much enjoyed
@MicroChemsExperiments
@MicroChemsExperiments 2 года назад
Welcome
@md.mostafijurrahman7561
@md.mostafijurrahman7561 3 года назад
Great, thank you. Can you upload detection test for salmonella species specific test ?
@MicroChemsExperiments
@MicroChemsExperiments 3 года назад
All of the test in this video are also applicable for Salmonella detection. ru-vid.com/video/%D0%B2%D0%B8%D0%B4%D0%B5%D0%BE-BGoueEDj6VQ.html
@md.mostafijurrahman7561
@md.mostafijurrahman7561 3 года назад
@@MicroChemsExperiments Thank you, very informative. What are the sample I can used? I want to use swab from life birds and egg yolk to detect salmonella spp is it possible by using your protocol.
@MicroChemsExperiments
@MicroChemsExperiments 3 года назад
Yes. You can apply this procedure for swab samples.
@md.mostafijurrahman7561
@md.mostafijurrahman7561 3 года назад
@@MicroChemsExperiments great...I will design a project protocol based on the methods...it would be appreciated if I get some written notes about the whole procedure/methods with list of lab equipment and chemical/reagent. Thanks
@MicroChemsExperiments
@MicroChemsExperiments 3 года назад
@@md.mostafijurrahman7561 Sorry. We don't provide any document. Please make a note from our video. Thank you
@SirajulIslam-vp9gp
@SirajulIslam-vp9gp 3 года назад
Thank you very much, Madam. May you help me...about Test method of Staplylocuccus Aureus or any video link.
@MicroChemsExperiments
@MicroChemsExperiments 3 года назад
Will upload soon
@my11account222
@my11account222 2 года назад
Are you a food microbiologist?
@SirajulIslam-vp9gp
@SirajulIslam-vp9gp 2 года назад
@@my11account222 Yes.
@my11account222
@my11account222 2 года назад
@@SirajulIslam-vp9gp thanks god, can i ask you some questions sir?
@SirajulIslam-vp9gp
@SirajulIslam-vp9gp 2 года назад
@@my11account222 ok
@rumisahoo6961
@rumisahoo6961 2 года назад
Pls mam.. Post Listeria testing procedure ..in Oxford media ...🙏🙏
@MicroChemsExperiments
@MicroChemsExperiments 2 года назад
We will upload it by the next week.
@rumisahoo6961
@rumisahoo6961 2 года назад
@@MicroChemsExperiments Tq mam 🙏
@MicroChemsExperiments
@MicroChemsExperiments 2 года назад
@@rumisahoo6961 Stay with us
@divya821
@divya821 2 года назад
Mam kindly givens in v cholera have whatreacion we can found in biochemical .
@MicroChemsExperiments
@MicroChemsExperiments 2 года назад
You can use these Biochemical Tests for Vibrio cholera too.
@divya821
@divya821 Год назад
Mam I have 45 yr female from fever case patient blood culture after growth only bap only rod and after biochemical test indole- ,urease+,cit-,tsi yellow with black ,and mha have mucoid around the antibiotics disc uti have greenish colony can u identify pls
@MicroChemsExperiments
@MicroChemsExperiments Год назад
Sorry we can't interpret this way
@divya821
@divya821 2 года назад
Mam can u suggest for cholera and salmonella confirmation biochemical test
@MicroChemsExperiments
@MicroChemsExperiments 2 года назад
This video is for Salmonella detection by biochemical tests. You can also apply for Vibrio cholera.
@rajuahmmed1257
@rajuahmmed1257 2 года назад
Please upload the 2nd part
@MicroChemsExperiments
@MicroChemsExperiments 2 года назад
Soon
@dulanjikarunarathna7853
@dulanjikarunarathna7853 2 года назад
plz upload the link of biochemical identification methods for bacteria
@MicroChemsExperiments
@MicroChemsExperiments 2 года назад
Part-2 will be uploaded soon
@mehedihasanrabbi342
@mehedihasanrabbi342 3 года назад
plz,different types of water test parameter,iron,zinc,chloride,mg,fluoride etc.
@MicroChemsExperiments
@MicroChemsExperiments 3 года назад
Noted. We will.
@mehedihasanrabbi342
@mehedihasanrabbi342 3 года назад
@@MicroChemsExperiments waiting for ur drinking water test parameter&procedure.
@MicroChemsExperiments
@MicroChemsExperiments 3 года назад
@@mehedihasanrabbi342 Thanks for your patience
@rakeshkumarsonti9319
@rakeshkumarsonti9319 3 года назад
Please do a video on Aflatoxin b1 test for aqua feed or on raw materials
@MicroChemsExperiments
@MicroChemsExperiments 3 года назад
@@rakeshkumarsonti9319 Noted.
@fariyahnaseem5184
@fariyahnaseem5184 8 месяцев назад
Y tsa? Y not direct ?
@islaserena1599
@islaserena1599 2 года назад
Hello. If I want to test for E.coli, what media is suitable as sources of inoculum for biochemical test?
@MicroChemsExperiments
@MicroChemsExperiments 2 года назад
Culture your suspected colonies on Trypton Soya Agar, Nutrient Agar or Plate Count Agar. Then used colonies for biochemical tests
@islaserena1599
@islaserena1599 2 года назад
@@MicroChemsExperiments Can I use Chromocult Coliform Agar for smear and then streak on TSA agar?
@MicroChemsExperiments
@MicroChemsExperiments 2 года назад
@@islaserena1599 Yes. You can. Where are you from?
@islaserena1599
@islaserena1599 2 года назад
@@MicroChemsExperiments Thank you so much. I am from Malaysia.
@MicroChemsExperiments
@MicroChemsExperiments 2 года назад
@@islaserena1599 Okay. Thank you for staying with us
@syednoorulmurtuza245
@syednoorulmurtuza245 2 года назад
i need your help for salmonella ,satph.aureus,e.coli0157,bacillus cereus for food microbiology analysis
@MicroChemsExperiments
@MicroChemsExperiments 2 года назад
We uploaded video for Salmonella and E. coli. We will work on Staph and bacillus soon
@syednoorulmurtuza245
@syednoorulmurtuza245 2 года назад
Ok Thank you very much
@syednoorulmurtuza245
@syednoorulmurtuza245 2 года назад
Can you share your Mobile Number plz
@jawharkhan7098
@jawharkhan7098 5 месяцев назад
Hello can you tell me about LiM medium LiM stand for ?
@MicroChemsExperiments
@MicroChemsExperiments 5 месяцев назад
LIM stand for Lysine Indole Motility
@jawharkhan7098
@jawharkhan7098 5 месяцев назад
@@MicroChemsExperiments thank you for timely response
@jawharkhan7098
@jawharkhan7098 5 месяцев назад
Can you recommend me 1 dilution Media 2 slective enrichment broth media 3 slective culture media for staphylococus aerus & pseudomonas ariginosa for food analysis?
@ajitpatil2153
@ajitpatil2153 2 года назад
Kya aap muze salmonella biochemical test kaise karte hai pdf mail lar sakte hai.. ???
@MicroChemsExperiments
@MicroChemsExperiments 2 года назад
Sorry we don't provide any document.
@syednoorulmurtuza245
@syednoorulmurtuza245 2 года назад
hello i am syed from bahrain medical lab
@MicroChemsExperiments
@MicroChemsExperiments 2 года назад
Welcome to this channel
@syednoorulmurtuza245
@syednoorulmurtuza245 2 года назад
Can you share your Mobile Number plz
@gracetanyaempasis1968
@gracetanyaempasis1968 5 месяцев назад
If we pay for identification of bacteria how would these cost?
@MicroChemsExperiments
@MicroChemsExperiments 5 месяцев назад
Please write us at mail.mic.chem@gmail.com
@realtimetraveller
@realtimetraveller Год назад
Genome sequencing video?
@MicroChemsExperiments
@MicroChemsExperiments Год назад
In future we will upload
@mehedihasanrabbi342
@mehedihasanrabbi342 3 года назад
free fatty acid definition,plz
@MicroChemsExperiments
@MicroChemsExperiments 3 года назад
Free fatty acids (FFA) are produced by the hydrolysis of oils and fats. Since FFA are less stable than neutral oil, they are more prone to oxidation and to turning rancid. Thus, FFA is a key feature linked with the quality and commercial value of oils and fats.
@prince_ranaofficial398
@prince_ranaofficial398 3 года назад
Thanks...oh hellow...where are you now...please answer me
@MicroChemsExperiments
@MicroChemsExperiments 3 года назад
India
@prince_ranaofficial398
@prince_ranaofficial398 3 года назад
@@MicroChemsExperiments may apse bangla mai bat karsat ta ho
@MicroChemsExperiments
@MicroChemsExperiments 3 года назад
@@prince_ranaofficial398 No. We dont speak bangla.
@prince_ranaofficial398
@prince_ranaofficial398 3 года назад
@@MicroChemsExperiments okey...one question...What it used Selenite Broth chemical test of microbiology
@MicroChemsExperiments
@MicroChemsExperiments 3 года назад
@@prince_ranaofficial398 Selenite Broth is used for the selective enrichment of Salmonella spp. E. Coli and other coliforms are inhibited in this broth.
@user-dt1bg4lu9c
@user-dt1bg4lu9c 11 месяцев назад
Wow You made it not simplified
@MicroChemsExperiments
@MicroChemsExperiments 11 месяцев назад
Not simplified? You mean hard? Opss sorry
@kuganeswarykali3539
@kuganeswarykali3539 Год назад
May I get Biochemical Test Part 2?
@MicroChemsExperiments
@MicroChemsExperiments 11 месяцев назад
We will make video later. Stay with us
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