Caffeine extraction from coffee ☕🗜️☕ 

Caffeine* and no, using ethanol would be dumb as heck. You just add extra steps (and solubility in EtOH is much lower which makes the method absolute trash ) Also who would even think of column chromatography or even mention it when the product can be recrystallized...

nope, you should try your own method and let us know how it works

You can always purchase a distillation set up! Id suggest to watch my latest video if you like distillations too

Nation Of Our Hobbies you're right. but probably that's so much work to do with those glasswares used in labs ^^ ... the amount of my rice wine is almost 10 liters! so i decided to make simple distillation tool by myself with some copper pipe and a big plastic basket and silicon hoses... but i love your distillatin videos so much i already watched and subscribe your channel. from Korea

Nice project! And thanks for subscribing to the channel, good to see people come from everywhere around the globe!

In fact is a purine itself! A trymethylated one by the way. So does theobromine which can be found on chocolate and has similar effects :D

Thanks!! Much appreciated!!

Thank you! It is the housebrand hehe

Nice to hear that!! If you liked it, check out my other chem videos too!

Thank!! But i dont know if I can do anything more with coffee ;(

Thank you¡¡ Apreciate the comment :D Yes of course, it can be done without adding HCl. I just wanted to follow a different way than the rest of the people. I didnt know how acid can coffee be itself. I wanted to add HCl because in other extraction I did previously, some caffeine were decomposed during the heating process (during the recrystallization I found that caffeine were dissolved in hot water but then never crystallized. In fact i let the water evaporate and I found some caffeine agglomerates in a viscous oil medium; and that was from relatively pure caffeine crystalls). By adding HCl I was able to increase caffeine solubility in order to not apply so much heat. Anyway, like it is written in the video description, I always want to know how my yield is compared with you, the internet people. Thanks for commenting :D

Oh, thanks for the explanation. It totally makes sense! Also, I believe because you are extracting from the nonsoluble material, HCL gives this extra bit of power to protonate and dissolve caffeine faster. In any case, well done! Will be looking forward to your videos.

I will never say it better. The HCl is exactly doing what you said, giving that extra bit of power :D Thanks for your support!!!

Thank you for watching!!!

Thanks you¡¡¡¡¡ Check out my other videos, maybe you like them too :D

wouldn't this extraction yield a full alkaloid profile? i know that the flavons and antioxidants would be stripped, but the trace amounts of non-caffiene xanthine alkaloids should be in that final product. i would like to see this done with Mitragynina Speciosis leaves, very closely related to tea but contains 7-OHM, a tryptamine like molecule that binds to mu-opioid receptors and produces hydrocodon like effects without danger or histamine release of traditional pain medicine

;)

Nation Of Our Hobbies Where do you get the vacuum filter set ?

Well, I get it by pieces in labbox.com It is not sold as a kit or something, I just connected few diferent pieces

Nation Of Our Hobbies OK Thank you

Thanks! Yes absolutely, I would have loved to have acces to an HPLC instrument to see how dirt and rich in other compounds my caffeine was. I asume it is mostly caffeine as in the last step a recrystallization is performed, but yeah, as you said, many other compounds can be present there. I have a video planed to purify it by sublimation, will upload it some day :D Thanks for commenting fellow!

Try to search it as pipette filler ;D

Ebay :)

Thanks for your comment! I am surely growing in numbers yeah! Many people ask me that lately. I didnt make any research about that but this is a more specific method, as I use some HCl and so... But can guess that it will work for other plan materials too!

Hi! I use Avogadro to make the molecules and its rotation and then use Fraps to capture the video. I just use some bright green or magenta background to then apply the chroma in the video editor ;)

Nation Of Our Hobbies OK thank you because i want to make a video with a molecule's animation.😊😊

It is a suctio adapter for a jointed buchner funnel. In this case with 14/23 joints both female and male

Hi thanks! I have a video planned for purifying it but with a much convenient method: sublimation. I may do a recrist after that to show you the crystalls yes, but the clip you see at 13:38 is pretty much it

Hii!! Suree, it is better in fact, less dangerous. In fact it is easier to crystallize from ethyl acetate by cooling a solution of it ;D Good luck in your extraction!!!

@@Glattuh Thank you for the very quick answer :)

@@Glattuh how about using acetone?

Thank you¡¡ Im moving to another place to record all this lab videos, will think about that ;D

Nation Of Our Hobbies Nice ;) Take an example of NileRed on RU-vid he has made videos on caffeine to

+Random Experiments International Thanks!!!

Well in theory you can but hydroxide by itself is a much more strong base and also nucleophile so it may degrade your organic compounds, not even caffeine but it can make the whole process much more difficult ;( From my point of view, carbonate is cheaper and more safe to use so its a goto in this experiment (and in many others for the same reasons)

I run a chem YT channel. But in reality i dont need it anymore, have a good sponsor that provides me with everything I need :D

Hi. This method is not to make coffee but to extract one if its compounds and purifying it. The liquid is no longer edible once it touches chemichals such as DCM which is an organic solvent. You can think I am """decaffeinating""" the coffee but, as I said, it is not longer edible, both coffee and caffeine extracted.

@@Glattuh why not? Even if a simple under-the-air drying does not remove all of the solvent from the crystal matrix of caffeine, it can be either dried even more deeply under a good vacuum to reduce the residual solvent content to a safe level, or washed by a food-safe solvent such as ethanol.

Hola¡ No existen no. HCl, acetona y metanol se venden en muchos supermercados como productos de limpieza, normalmente en forma diluida El metanol por ejemplo forma parte de lo que se conoce como alcoholes de quemar (de coloracion azul). El carbonato de sodio no entraña ningun riesgo y tambien se vende para limpieza, con algunas otras aplicaciones. Un saludo ;D

@@Glattuh en argentina, aun siendo farmaceutica, hay restricciones de compra, una verguenza. gracias x tu respuesta

Well, I am a bit confused. People there prepare coffee not caffeine. Caffeine is the main alkaloid responsible of making you active and excited. Caffeine is extracted industrially by superfluid extraction. This is done by chemists :D

I wish I could haha. It is my style I guess! About the other comment, well, this videos have time actually, but thanks for commenting. Check the new ones if you are interested! Glad to see you discovered my channel, thanks for stopping by! Bests, NOOH

@@Glattuh For sure. This is a great video! Much more detailed than most other caffeine extraction videos. Maybe try doing a similar one for extracting piperine from black pepper? Or extracting capsaicin from ghost peppers? Just a few ideas. Regardless, keep it up! I plan on starting a channel similar to yours sometime in the near future, hopefully. I have a lot of cool chemistry experiments that I dont see covered very well in YT videos. Thanks again bud.

Yess I will do them for sure!

@@Glattuh Do you know anything about _pseudohalogens?_ I think that could make a cool video... Showing how a compound containing a halogen could have the halogen substituted with its pseudohalogen but still have nearly identical traits/attributes. Learned about them when researching Fulminates

Wow never heard about them! I will look for it for sure, that is not a common thing I guess

Hi! Caffeine needs to be in basic aqueous conditions in order to be extracted because it is an alkaloid, which among other things means that it is alkaline or basic. If an acid is present it forms a salt which goes to the water phase instead of the organic one. By addind a base, in this case a carbonate, we force it to be in its free base form, which can go to the organic phase in a higher propotion.

Well the quick answer is that regarding of its low content it is powerful enough to make you excited, awake and even nervous, and that can lead to health issues of course. Just to mention, these are my own results and are based on dry coffe grain, not the liquid you drink. And thanks for that!!

Hi¡ No it isnt. Nothing is food grade in my opinion if you made it by yourself instead of a certified laboratory or company. I used organic solvents and also an acid to recover it. The video is just to show you how I extracted the caffeine ;D

Chloroform is the same as DCM, but whit one chlorine atom instead of an H. DCM and chloroform are pretty similar and both will dissolve caffeine, so yes it can be used.

You can not! For liquid-liquid extraction you need two liquids that are not miscible with each other. Acetone is miscible (soluble in all concentrations) with water so you wont observe a separated phase

@@Glattuh how about using ether?

That's another story! Yes ether can be used too

@@Glattuh thanks for answering late quation

Oh boy, drinking coffee with HCL? Good luck

Thanks for commenting! I am glad you found a pattern across this method on the internet. What is clear is that the method of extraction is not so effective, plus, it could be a mix of the other variables you mentioned. On the other hand keep in mind that this is not an analytical type of chemistry, just an amateur extraction. If you really want to know how much caffeine is within certain coffe, you would need another method, techniques and work with very small amounts, not a whole kg as I did.

Nicotine is a volatile liquid at room temperature, and gram-for-gram a ton more toxic, so not the best idea. It also readily oxidizes on contact with air into cotinine. Solvent extraction of nicotine generally works by placing finely-shredded tobacco leaf in a base solution (as the nicotine present in plant material is generally a salted form, so this liberates the freebase), then performing a steam distillation using super-heated steam injection (around 250C), and the resulting nicotine freebase dissolved in a suitable carrier (eg. kerosene). Then it's cleaned and re-acidified for transport (as the salts are generally less volatile). Again: Nicotine is /insanely/ toxic relative to caffeine on a weight basis, and it's a volatile liquid. If you have to ask how it's done, you shouldn't even consider attempting it.

@@AikkuKun Thank a lot. but I need to extract nicotine for e-cig ( I want to quit smoking). Can I use ethyl alcohol instead of kerosene?

@@kongkiatc If you need nicotine for e-liquids, you buy it pre-made (generally 12-24mg/mL when ready-to-use, 80-150mg/mL for diluting with PG/VG/PEG/DH2O, etc.). Concentrated (and especially: pure) nicotine should only be handled by skilled chemists in professional laboratories with all safeties in place. I seriously cannot stress this enough: *Nicotine in its pure and/or concentrated forms (and especially as salt) is absolutely /deadly/; do NOT play around with it.* It should never even be present outside of sealed environments in a professional laboratory or processing facility. Even tiny amounts (2-3mg) are enough to make you sick, and because of how volatile it is (especially during the steam distillation), you would be exposed to a lot more than this without professional equipment and training, and you might not even realize it until too late, at which point you may collapse and need urgent medical help to avoid organ failure. I only answered as a curiosity in regards to the chemistry in play; it was - and is - NEVER meant to be performed, and is only for educational purposes. All that out of the way, and again *only* for educational purposes: Freebase nicotine appears to be somewhat soluble in both polar and non-polar solvents but moreso in polar ones (ie. water), which may explain the need to use superheated steam distillation; EtOH would 'work' about as well as any other solvent, but I imagine that it would also evaporate the nicotine if you attempt to dry it (it might be possible to dry a solid nicotine salt under vacuum, but most of them are liquids afaik, and I'm not sure how stable they are; they might decompose into freebase + acid mists).

@@AikkuKun Thank you very much for the advice. And that worried me I will follow your advice.

@yabon banania I eventually googled it. According to science direct its dichloromethane and its a common solvent for extraction.

A scale has a maximum capacity. So one can only weigh this minus, well the tare. Brutto (capacity) minus tare (the container even a piece of paper for powders etc.) makes netto (what you actually weigh). In other terms a scale is not a calculator even with the count button. Maximum capacity still applies.

How would this process look? I am looking to do a school project

Thanks for the idea! Will be an interesting video to film :D

In fact, I extracted a lot of caffeine using this method. I did not found any video at that moment that extracted 1000mg of the desired compound :D

read much?