cDNA Synthesis Protocol by Reverse Transcription 

I was so confused with these terms and you explained so clearly and so well. Thank you so much

Thank you soooo much for this. This thing was more like rocket science to me before watching this video. Cleared all the concepts in just one go. Thanks again. 😊 Have great day ahead

Merci pour la vidéo, elle est très claire et parfaite pour bien comprendre le mécanisme de la retrotransciption ! Thank you !

Thank u so much. before I watch this video I read some what notes but they were boring. ur video were so interesting with smooth sound and full information.

Thank you we need more explanation in different Lab techniques. It is practical and we know need to know the sciences. Most of the time I find myself confused in class but thanks to you now I understand it better. Keep it up we need you. You deserve all the success.

Sehr verständlich, vielen Dank!

Thank You So much Sir.. I watched lot of Videos But No one explained about RNaseH Function and I was really confused. You Really Explained very nicely. Thanks for making this video..

Excellent explanation & graphics. Keep up the good work!

Thank you so much, Sir! the explanation is to be so clear and easy to understand for us.

This is great. Perfect rythm. Thank you.

Very helpful! I finally understand what do we actually use reverse transcription for, thank you!

Sir I have lack of word to thanks you. Really you I m very excited after watched this . Stay bless forever God bless you More and more forever ❣️💝

Excellent video. You made this concept easy to understand, thank you.

Fantastic! Amazing! Incredible! Astounding! Mighty! Wonder! Super!

Thank you so much. The explanation is very intelligible.

Very good explanation man. Thank you so much!

Very well explained concepts are clear about cdna

i was confused for intron thingy and u made it easy

Thank you so much Henrik's Lab. You are doing a very noble job by providing us such useful concept clearing video. Through your videos we are able to understand the topic better than hours of classroom learning. ❤❤

Thanks you so much finally got what i was looking for

This helped me with my genomics class 🔥🤙🏿

Excellent explanation!

it is very nice and please adding brief explanation about recombinant DNA

Muito bom o vídeo, parabéns 👏🏽👏🏽👏🏽💚

My doctor should watch this srsly

Muy buen video! Gracias!

Great explanation!

Keep up the good work

amazing explanation. cheers mate

Amazing! Thanks!

Thank you so much sir❤

thank you very very much!

Thank you very much..

Thank you ❤ God bless you ❤️

Nice explanation ❤️

amazing , keep going

How do you make cohesive ends on the cDNA so you can be able to ligate it with the chosen vector?(using restriction enzymes for the cDNA given would cut out some of the coding sequence right? so how do you add cohesive ends? thanks

Thank you 😊💕💕

Hi, i hope someone will help you the way u helped me today, I wish u the best from France

Best video ever❤️❤️

Thank you so much!

Hey, one question after the synthesis of ds-cDNA. How it will multiply in numbers.................Without having the primer for coding another strand....?

Hello, i am facing problem in cdna synthesis. would you please help me to know your protocol?

Mam 5'AGUCAGGUC3' this is mrna what is the C dna to this sequence

🙏thank you.

Danke, brudi! Arbeiten grade an einer präsi über Gen Chips und das ist verdammt nützlich!!! Love you

Thank you

At 3:20 the last exon shouldn't have blue "legs", because blue was polyA before. Poly A is not in DNA.

Thanks!!!!

Thanks :)

Today, my Professor explained this topic to us, somehow she explained, that after the synthesis of the first cDNA-Strand the Double-Strand would be denaturated into Two seperate ones, later there are inhibitors to Stopp the accurance of Rnase. Later Taq-Polymerase is Synthesizing the other Strand from the cDNA-Strand that was reverse trnascripted before, not like in your video by Nicking from the Rnase and Polymerase 1 and later with Ligase. I asked her about this and she thought that you explained it as an example how it would happen in the body not like in Biotechnology. Im got super confused that she explained it different at some point. Weirdly enough she suggested us your video to learn the reverse transkription, thats why i confronted her today, in order to get clear what is correct for her exam XD Maybe the is some potential to make a different video out of it? Danke für das gute Video. Hab es dennoch gut verstehen können!

Is there no hairpin loop?

Thank you. How can we contact you if we get problem while doing qpcr wirk?

Why cant we use mRNA directly in microarray or qPCR

in 2:57 may I ask how whole mrna are replaced by the DNA? thank you

Is there a way we could translate all the proteins in a human genome and create a lab created human since the entire genome has been sequenced?

Wow! what can I get you from the vending machine

😃😃

I have a question. What´s the meaning of the last product in this process? what is "ds"?

Can you Translation.. to Arabic

Bist du deutscher ? So hörst du dich an XD

deutsch wär echt cooler gewesen

Wait a second people...forgive my lack of understanding but i dont understand why start to syntettize anything about and HUMAN DNA??? Why want to know what GOD did once and why and how and how to REVERSE IT/////?????!! Please someone explain..Many thanks

Bruder sprich doch deutsch

Man trying to play God not our place to mess with God's code that makes us human.