Reverse Transcriptase PCR (RT PCR) | What are the applications of RT PCR ? | RT PCR protocol 

Sorry, I can't give a 'super thanks', but I would like to express my sincere thanks for your videos. I am able to understand a lot about biotechnology and then passed my exam. I'm a vet student from Indonesia. 🙏

Thank you so much for these very useful short tips!

Hello Bro You have not included the further steps Like After making the hybrid rna-dna .The hybrid's rna is partially digest by Rnase H ,then DNA polymerase I forms a complimentary strand and nick is sealed by DNA ligase and Thus forms a cDNA

Very well explained thanks !! The animations are really good

Sir is this technique used for hiv detection

very good, detailed and concise explaination, thank you sir!

Very good info! Do you have the references??

you saved my todays prentation sir, thank you very much!

Sir can u explain about multiplex pcr

I can't thank u enough for this... just keep going 🙏🙏🙏

Amazing explanation ... thank u sir...🙏🙏🙏🤗🤗🤗🤗🤗🤗

It was very helpful ❤thank you sir😊

Great video, comparison of RT-PCR and PCR was a benefit

Please upload a video on allele specific PCR.

I have a question: when I have transcribed the mRNA into cDNA, do I perform a normal PCR with the cDNA? Can someone pls tell me if i'm right or wrong ? PCR steps 1) denaturation of the template DNA 2) use primer and poymerase 3) amplification RT-PCR: 1) isolation of RNA 2) transcribe with reverse primer and enzyme reverse transcriptase into cDNA 3) follow PCR steps

Hi thank You for the well explained video can you explain microRNAs

Great help on exam eve😇🤩

thank you

Thank you so much sir.

very nice channel thanks from turkey arpan

Thanks, good content, should give you thumb up.

awesome

sir is rt pcr is same technique used in covid rest or it is different .

Thank u sir

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can u plz explain tma and nasba

Dont we use a specific primer in RT pcr with those 2 primers?

Yawrrr such a super duper lecture thank you so much🥹✌️

Inverse pcr k upar ek banayega plz

Hello sir, i want to go for research but i don't know how to find research institute for my topic of research can u help me that how can i search institute.

What if we don't use hexamer primers???

Can anybody tell me if reverse transcriptase posses 5'-3' exonuclease activity then why aren't Hexamers degraded instead they are incorporated 😢😢

hello dear. The RT-PCR consists in 1 cicle only ??

Where does the reverse transcriptase itself come from?

thanks alot

505 Muller Mission

if you claim to be knowledgeable ...... is this a real quotation or not? “Quantitative PCR is an oxymoron.” Kary Mullis, inventor of PCR. As it it is then almost all the stats we have about Covid are bunk.

Wunsch Extensions

References please🙏

Please refer the references i need to write my notes thanks in advance

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