Compass Blots: Northern, Western, Southern, & Eastern | MCAT 

TL;DR: Compass blots assay for different molecules: Western - protein, Northern - RNA, Eastern - post-translational protein modification, and Southern - DNA.
Today’s MedCat video covers a variety of different so-called “compass blots,” named for their specificity for various types of molecules. Western blots assay for the presence of proteins, often with the use of a labeled antibody and electrophoresis. Northern blots often use radiolabeled probes to assay for DNA; similarly, a Northern blot uses probes to look for RNA. These are the highest-yield compass blots, though we do go over Eastern blots, which assay for post-translational modifications (e.g. phosphorylation, glycosylation, and prenylation) as well as a Southwestern blot, which unsurprisingly is a combination of a Western and Southern blot in that it assays for DNA-binding proteins. Finally, we finish up with a AAMC-derived practice problem.
Link to research article by Hitosugi et al. on phosphorylation of pyruvate dehydrogenase: bit.ly/3wJC7Au
Time Stamps:
Intro: (0:00)
Northern, Western, Southern, Southwestern, and Eastern Blots: (0:08)
AAMC-Derived Practice Problem: (3:28)
Outro: (4:41)
Comprehensive Amino Acid Playlist: bit.ly/3sMGBUG
Check out Aratasaki, the beat maker behind my intro and outro: bit.ly/2Pma5v0
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#mcat #compassblots #westernblot
Compass Blots: Northern, Western, Southern, & Eastern | MCAT
Compass Blots: Northern, Western, Southern, & Eastern | MCAT
Compass Blots: Northern, Western, Southern, & Eastern | MCAT
AAMC FL (full-length) 2 B/B (Bio/Biochem) #23