DIY Liquid Culture Luer Lid for Fast & Contamination-Free Extraction

Подписаться 3,4 тыс.

Просмотров 10 тыс.

50% 1

Welcome to Real Simple Mushrooms, where we make home mushroom cultivation easy and efficient! In this video, we'll guide you through creating your own Liquid Culture Luer Lid for mason jars. This specialized lid is designed to minimize contamination risks and streamline the process of extracting liquid cultures into syringes. Say goodbye to tedious procedures and make your home cultivation journey smoother!
🍄 Key Features:
Contamination control for healthier cultures
Faster and more efficient liquid culture extraction
Easier on the hands for hassle-free syringe preparation
🔧 Tools & Materials Used:
2" Step Drill Bit: amzn.to/48lycx4
1/4” PP5 Bulkheads: amzn.to/48ivbOb
1/4” PP5 Female Luer Hose Barb Adapter: amzn.to/3tDkSFf
1/4” High Temp Silicone Hose 10’: amzn.to/47DvSR3
6pk Leakproof Widemouth Mason Jar Lids: amzn.to/3tIbW1f
PP5 Dual M/F Luer Lock Caps: amzn.to/4225Qpr
10pk Luer Lock 3 way Stopcocks: amzn.to/3tM4br2
High Temp RTV Gasket Maker: amzn.to/47w7vEQ
🌱 Benefits of Using a Liquid Culture Luer Lid:
Improved success rates in liquid cultures
Reduced risk of contamination during extraction
Time-saving and user-friendly for both beginners and experienced cultivators
🤔 Have questions or suggestions? Drop them in the comments below!
👍 If you found this video helpful, don't forget to like, share, and subscribe for more Real Simple Mushrooms tutorials. Happy cultivating! 🍄✨
Chapters:
0:00 - Intro
0:25 - Why Build These?
1:57 - What You'll Need
4:05 - Assembly
7:51 - How to Use

Опубликовано:

30 июл 2024

Ссылка:

Скачать:

Готовим ссылку...

Добавить в:

Мой плейлист

Посмотреть позже

Комментарии : 116

@radagastjones9339 2 месяца назад

The links to the parts are serious time savers, and the knowledge to build these extraction lids myself is a serious money saver. You have done us all such a great service with the concise, excellent little video. Thank you very much!

@azarbarzincompany5319 16 дней назад

Hello my friend. I am an experimental mushroom researcher from Iran. I found much simpler ways than you, which increases the speed of your work in the production of mushrooms, especially in the stage of making agar plates and sowing seeds, as well as in the production of mushrooms. These methods are used for both types of food and medicine and magic. I have many achievements in this field that are rarely mentioned anywhere. For example, the effect of the type of seed on the fertility and the creation of mushroom contamination, or the formation of hybrid and hybrid breeds, I mean the integration of mushroom breeds with each other and the creation of breeds that did not exist until now.I think I am creative and thoughtful in this field and I have a personality to communicate. I am looking for friends like me who are curious like me. Let's learn and teach from them

@jakeyoung7567 19 дней назад

This was extremely impressive.

@metamike2012 6 месяцев назад

I'm glad someone finally covered this. I'm in the process of doing this in a couple days. I was wondering if all the parts were autoclavable. Thanks for finding out for me. I plan on using a one-way check valve instead of the stop-cock. Until now I used 8 gauge metal dispensing needles, but epoxied them to the lid so they couldn't spin or move. I also use UV glue wherever I can. Keep making great videos, we need them.

@RealSimpleMushrooms 6 месяцев назад

Hahaha, only one way to find out what works in the PC right....lol Glad you're finding the videos helpful. I do enjoy making them.

@williamvallejo5976 3 месяца назад

Yeah, well it was kind of crazy because while it was chugging along at 15 psi, one of those red caps popped off of 1/4 LC jars I made and it was shootin steam out the top of the jar hose so much so that it made the pressure force It's way out the weight valve I almost jumped out of my seat because I'm a quadriplegic 😂but that one jar it happened to was like 150 mL low so it was like a mini little pressure cooker, but inside of a big pressure cooker 😱😱

@goodtoknow1331 2 месяца назад

Why not just use regular lids during the PC and then swap them out for these in front of the laminar flow hood after ?

@willbennett8412 6 месяцев назад

Your videos are a huge help. Thanks!

@RealSimpleMushrooms 6 месяцев назад

Glad to hear it! Happy to help!

@Kevin7G 6 месяцев назад

This is that 2024 content that we need. Liked and subscribed, thank you so mush! Mush love 🍄

@RealSimpleMushrooms 6 месяцев назад

Thanks for the sub!

@roaner4313 4 месяца назад

Ordering all this stuff right 😸 meow! Thanks for all the info

@RealSimpleMushrooms 4 месяца назад

Any time!

@gregorynancekivell4678 6 месяцев назад

Thank you- very helpful.

@RealSimpleMushrooms 6 месяцев назад

Glad it was helpful, and thank you for the kind words.

@GreatfullySeeded 2 месяца назад

thanks for the walk through! 🎉

@RealSimpleMushrooms 2 месяца назад

No problem!!

@jakeyoung7567 19 дней назад

Just wow.

@rawforaging 6 месяцев назад

amazing thank you x

@RealSimpleMushrooms 6 месяцев назад

You’re welcome 😊

@DEFILEX 6 месяцев назад

Thanks for doing this, so hard to find quality instruction on how to make these lids

@RealSimpleMushrooms 6 месяцев назад

Glad it was helpful! They're so easy to build, and people are out here charging $15-$20 per lid and they aren't even as nice as these lol

@linemanlovesyou859 5 месяцев назад

Beooooooo I was just wanting a tutorial on this exact thing!

@RealSimpleMushrooms 5 месяцев назад

Awesome! I love these lids

@nickdeborbon4947 5 месяцев назад

super helpful Content. i have 4"x14g Stainless needles im currently using for my LC, and this is a great way to not need sharps while also being able to reach all of the LC in the jar with less flame sterilization. can just buy blunt fill needles in bulk for inoculating Grain

@RealSimpleMushrooms 5 месяцев назад

Glad it was helpful!

@KOKO-uu7yd 6 месяцев назад

I'm very new, and have had plenty of "learning opportunities" already😂 Just this weekend i worked with lc jars for my first time (i did inoculate them myself, but getting it out of the jar... oof) I made plenty of mistakes! These will address SO MUCH of the challenges i had. Thanks!

@RealSimpleMushrooms 6 месяцев назад

You got this!

@KOKO-uu7yd 6 месяцев назад

@RealSimpleMushrooms Thanks mush! One thing I do have is stubborn tenacity (or aggravating bull-headedness, depending on who's asked 😈.) (Left a new question for you, just in case it's something others would find helpful.) ❤🍄❤

@art755 6 месяцев назад

Thanks

@RealSimpleMushrooms 6 месяцев назад

Sure thing!

@JUSTINOURSE 6 месяцев назад

I made Some last week and I was putting aluminum foil over the draw a tube cuz I couldn't confirm that the red tips were autoclavable.. Thank you so much! I'm really loving your style of content!

@RealSimpleMushrooms 6 месяцев назад

Glad I could help

@PPP-on3vl 6 месяцев назад

@RealSimpleMushrooms do I need to sterilize the valve with alcohol after each use ? Could u pls show this how u handle it

@RealSimpleMushrooms 6 месяцев назад

So for me, when my jar is ready to extract I prefer to pull it all in one sitting. Once the mycelium has colonized the jar to my liking I will empty it into as many syringes as it will fill. If you're only filling a couple you can try to sterilize with ISO, or you just put a fresh sterile stopcock on each time (which will cost more down the road).

@malekith8215 4 месяца назад

Great Video Very Good Idea I am growiing in the tropics where contamination is a huge problem I will try that solution. One question though. Is it possible to also inoculate the LC through that silicon hose? Or will too much stuff get stuck in the hose?

@philipholman9898 3 месяца назад

Found everything but the pp5 lid, great video, I'm going to try this out . Thanks. Amazon does have brass and stainless steel stop cocks. I'll use the plastic ones I think though

@RealSimpleMushrooms 3 месяца назад

You should be able to find those ball brand lids at Walmart

@williamvallejo5976 3 месяца назад

I bought everything in the description and did everything according to this video… Everything went great, except for the little red cap with the Luer lock melted and had aluminum foil covering as well, so what did I do wrong? 😂

@RealSimpleMushrooms 3 месяца назад

No, you didn’t do anything wrong. Those Lids seem to be a little hit or miss. I’ve had some survive and I’ve had some melt, I’m looking for a better solution now.

@bradsmith3346 3 месяца назад

@@RealSimpleMushrooms there are nylon and PP ones on McMaster. Did you have problems with contamination after the plugs melted? Or should I be good to just swap the caps out while I wait for new ones to come?

@bevwilliams2105 3 месяца назад

First, this was a great video.. THANK YOU ! Second, I just did the same and had the same issue with the red cap. I went back to Amazon and read the reviews, and older reviews didn't mention an issue with the caps but more recent ones did. I had just ordered new caps from a lab supply company and will reprocessed the jars when they are delivered. Not the end of the world , just annoying.

@SporeWhore661 4 месяца назад

Use a high temp flow control clamp, that way you can pc the whole setup.

@user-hp2hx2ev9k Месяц назад

The red stop caps melt got them from your link made 3 jars spray loc in the pc

@sauceionjiggy7893 3 месяца назад

Where can I get the injection port and what type of port do I need? Can I use the high RTV gasket maker?

@MichaelMorana 4 месяца назад

I was having trouble sourcing female luer to 1/4 barb adapters available on Amzn that were polypropylene. I opted with male adapters which will still connect with a 3 way stopcock and leave 1 connection available for a syringe at the stopcock. Throwing this solution out there to anyone having supply issues with the female adapters rn.

@nandodando9695 Месяц назад

2:42 polypropylene lids for pressure cooker safe.

@irate74 10 дней назад

Very interesting, does that stopcock leak over time? That'd be my only concern because if it doesn't stay air tight at all times gravity will pull the liquid down and draw in dirty air

@drdr3d3d 2 месяца назад

Sorry for thr seemigly stupid question but is a 3 way stopcock essential? Could i use a 2 way one ?

@doctorali53 6 месяцев назад

Instead of home made jars, you can use dextrose water 5% serum, medical grade sterile, with self healing injection port, and use serum set for injecting and aspirating, you could buy from a pharmacy

@RealSimpleMushrooms 6 месяцев назад

There's a variety of ways to accomplish this, the point of this video is to use readily accessible items anyone can get their hands on.

@KOKO-uu7yd 6 месяцев назад

You're talking about iv saline bags. Yes? I can see that being effective and easy. I would prefer to leave that much medical waste for medical purposes, though, if possible. There's also the cost and the volume. I'm not going to need THAT much culture for any one strain, not for a LOOOONG time, anyway. I'd also have to figure out storage. Combine that with the cost per bag (that I've found so far, anyway) and unless I have a family member with "sticky fingers" working in a hospital setting (and I was willing to do that), that could get pretty costly kinda quick. I'll try these reusable forms. I'll keep in mind the bags as a backup, though. I can actually see them being very organized and efficient in a larger, professional setting even. For a home or small-scale mycology enthusiast, though, I think these will tend to be the better option overall. I also will be trying to minimize how much landfill I produce per "grow" where and when I can. All that said, if you have sources for smaller bags or for what you see as more cost effective, please let me know? I'd appreciate it! Good thoughts. Thanks! Agapé 303

@RealSimpleMushrooms 6 месяцев назад

No, I would just take up some sterile water in a syringe, then use the needle to biopsy the mycelium, (push the needle into the agar which should pick up enough mycelium) then just use the sterile water to push the myc into the jar.....does that make sense?@@KOKO-uu7yd

@doctorali53 6 месяцев назад

@@KOKO-uu7yd fyi, 5%dextrose water bags, not salin ones, they come in a verity volumes, 300ml up to 1000ml, these sugar bags are relatively cheep, sterile, equipped with self healing injection port, easy to buy from a drugstore , mostly come with sterile serum set which could coupled with syringes and cross connectors for easy usage, could be stored in cabinets for long periods of time, could save time and energy compared to home made sugar jars, with much much less risk of failure/contams. On the other hand, you may get satisfied if you make your own liquid jar while doing mycology as a hobby at home.

@KOKO-uu7yd 6 месяцев назад

@@RealSimpleMushrooms It does, i think, for my other posted question? I'm commenting so much im getting confusing, i think! 😂 So yeah, I think you're talking about using the cockstop to inoculate? If i understand correctly, something already liquid should be fine. If I'm working with something like agar or spore prints, I'll still need to have a "carrier" of sterile solution. Other than that, I should keep itnsimple and not over think. I got thst all about right?😂 Also, THANKS! You've taken some considerable time and responded quickly, to boot! Mighty kind😊😊😊😊😊

@danrampage01 2 месяца назад

Hi I’ve been using these lids a couple times now and they’re the dream. Just wondering if it would still be sterile to refeed through liquid culture through the luer lock with PC liquid culture solution syringes? Or have you already tried this?

@GGg-fx2fn 3 месяца назад

Guys, I can't find the (PP5 Dual M/F Luer Lock Caps) on Amazon to be able to put them in the pressure cooker

@bloodclot 6 месяцев назад

fyi: Theres many reports on the Dual M/F Luer Lock Caps that say they are not autoclaveable. is this url the same product as shown in the video?

@RealSimpleMushrooms 6 месяцев назад

So, I’ve had some survive and some not….

@MyCoskis-lh2yn 6 месяцев назад

Great video but quick question, how do you think inoculating through the stopcock instead of through the injection port would go?

@RealSimpleMushrooms 6 месяцев назад

If you're inoculating from another LC it would work just fine. You could also sterilize some water, draw some into a sterile syinge and then use the needle to basically biopsy an agar plate then inject as well. Just need enough of a sterile carrier fluid so that some mycelium makes it all the way down the pickup tube.

@MyCoskis-lh2yn 6 месяцев назад

@@RealSimpleMushrooms the exact reply i was looking for, thanks my guy keep doing what your doing we appreciate you for it

@badseedkramer 3 месяца назад

What would you be injecting into it

@gustavorodrigues6882 4 месяца назад

u can also do it with a needle and stopcock

@RealSimpleMushrooms 4 месяца назад

Yes you can

@danrampage01 2 месяца назад

This is awesome matey!! any reason you chose 3 way stopcocks over 2 way???

@RealSimpleMushrooms 2 месяца назад

They were the most readily available on Amazon tbh

@danrampage01 2 месяца назад

@@RealSimpleMushrooms yeah I’ve analysed your methodology here and it’s really well thought through. With the 6mm ID on the silicone tubing it’s evening amazingly well suited for SSLC, take a peep if you haven’t already come accross it ;)

@taskos1261 5 месяцев назад

What about that tiny amount of lc that remains above the 3 way valve ?

@RealSimpleMushrooms 5 месяцев назад

Me personally when my LC is ready I will empty the entire jar into syringes in one session. I really only like to access the jar once.

@SK-ql5bx 3 месяца назад

Is the stopcock necessary? What if you just took the red luer lock off and hooked the syringe straight to it without the stopcock? Also, how is the stopcock cleaned and used again for next jar of LC? Is this a one time use? Since you cant autoclave it, how do they get sterilized?

@RealSimpleMushrooms 3 месяца назад

It’s not necessary, I like to use them in case I don’t empty the entire jar into syringes in 1 sitting. As far as re-use they are a 1 and done deal.

@RutikPatil-wc1yt Месяц назад

how do you stir it? will stir bar still work with tube ?

@RealSimpleMushrooms 25 дней назад

Yes the stir bar work with the tube, you may just have to offset the jar on your stir plate a bit

@cmrclean 5 месяцев назад

Do you use magnetic stir bars and does it get in the way?

@RealSimpleMushrooms 5 месяцев назад

Yes I use stir bars, the hose usually has a small curve to it and you can move the jar slightly when stirring to avoid hitting the pickup tube. They work just fine

@cmrclean 5 месяцев назад

@@RealSimpleMushrooms amazing man thanks for the tip. Excellent video and thank you for your prompt response. I am in the middle of expanding my LCs and definitely ordering the items from your comprehensive list thank you for taking the time to do that I really appreciate it.

@rawforaging 6 месяцев назад

now that weve covered the basic stuff, how about we get more in depth and talk about recipes for cultures, good substrates for mushrooms and how different species have different needs. I've heard you can just use honey and water as a liquid culture recipe.

@RealSimpleMushrooms 6 месяцев назад

Honey, dextrose, Karo Corn Syrup....lots of stuff. I'll be doing a video on the recipe that I use very soon!

@KOKO-uu7yd 6 месяцев назад

Question for you (I'm rewatching now) - you have an injection port, but could one inoculate with lc or spore syringe via the stopcock? Perhaps a flush of sterile H²O would be needed to get everything down the tube and into the medium... 🤔🤷‍♀️ I'm not sure of this would ve an advantage or not, or even if it would work? Your thoughts? Thanks again!

@RealSimpleMushrooms 6 месяцев назад

Absolutely you can, I put the port there just to have both options.

@RealSimpleMushrooms 6 месяцев назад

you would certainly need some sterile water in the syringe as a transport mechanism.

@KOKO-uu7yd 6 месяцев назад

@@RealSimpleMushrooms I wasn't sure if a couple drops would be enough... like with LC. But, I can always try 😊 Thanks again!

@RealSimpleMushrooms 6 месяцев назад

as long a some mycelium makes it in the broth you should be good. It doesn't take much.

@justinkurtz13 Месяц назад

What is the correct size drill bit for this? I ordered the one from the list above and it's a huge drillbit that is much bigger than yours.. just letting everyone know and wondering which is the exact size to order? Thank You

@RealSimpleMushrooms 25 дней назад

The bit I use is a step bit, each step is a different size hole

@judyhowell7075 2 месяца назад

My culture is so thick, it’s been refrigerating while using.

@jha1425 3 месяца назад

I made a very similar one, but I didn't use a 3-way tap. Is it really necessary? Any reason not to connect the syringe directly to the female?

@RealSimpleMushrooms 3 месяца назад

I only used the 3 way because they were readily available on Amazon. The only reason I wouldn’t connect it right to the female on the bulkhead is storage if you don’t pull all your LC into syringes in one sitting. The stopcock gives you the ability to close it up and store it without worrying about contamination

@danrampage01 2 месяца назад

@@RealSimpleMushrooms once closed up and stored to prevent contamination, will a new stopcock need to be installed if you wanted to use the culture on more than one occasion?

@yorks6181 2 месяца назад

Those pp5 lids are not pressure cook safe, I just built this exact setup 25 minutes in the pressure cooker deformed and melted the lid. Gotta buy another pack now😢 other than that I’m excited to try out once I get some more

@RealSimpleMushrooms 2 месяца назад

The first pack I bought survived, after that not so much :(

@badseedkramer 3 месяца назад

Why would you need the injection port if you have the tubing

@RealSimpleMushrooms 2 месяца назад

Sometimes I will expand a current syringe, and sometimes I’ll use it to pull a test syringe to put on agar

@trip9919 3 месяца назад

You did not list the injection ports in the materials and you did list syringe filters but did not go over anything about them in the video but had the jar with them on one. So exactly why did I purchase them if I'm not using them and you didn't show what they were for?

@RealSimpleMushrooms 2 месяца назад

So I use the injection ports for 2 reasons. 1. in case I want to inoculate the LC jar with a current syringe I know it viable and clean 2. Sometimes I will pull a quick sample through it to agar test it for contam.

@dantefuego 5 дней назад

so after you pressure cook the Liquid Culture Nutrient, you'd wait for it too cool off and then inject the initial LC through that injection port ?

@RealSimpleMushrooms 4 дня назад

Exactly!

@dantefuego 4 дня назад

@@RealSimpleMushrooms Awesome! Will that filter patch suffice for air exchange? A friend of mine told me to never fully seal jars because its probable it can burst in the PC. I'm wondering if the filter patch mitigates that risk

@RealSimpleMushrooms 4 дня назад

I’ve never had an issue and I’ve PC’d hundreds of them

@ShiboTheGodfather 7 дней назад

Hello, how do you sterilize everything?

@RealSimpleMushrooms 4 дня назад

So after I build them I’ll run them through my dishwasher on the sanitize setting then I will put them in a mushroom grain bag and PC them for 60 min at 15psi. I will leave them in the bag until I’m ready to use them.

@bradsmith3346 3 месяца назад

So the red caps are in fact NOT safe in a pressure cooker. Have you tried the ones from McMaster?

@RealSimpleMushrooms 3 месяца назад

I've had some survive and some not, so yea a little bummed about those. I will look into the McMaster ones, thanks for that!

@bradsmith3346 3 месяца назад

@@RealSimpleMushrooms mine were so melted it was a major pain to get them off and replace them. Hoping I dont get contam as I replaced them while on sterilized jars

@sauceionjiggy7893 3 месяца назад

Where can I find these McMaster caps 🧢?

@stephenwalther9702 6 месяцев назад

Your information is amazing! Thanks so much for your information especially where to get the items on Amazon. My question is this: if you don't want to use up all of the liquid culture, can you put a cap on top of the stopcock, after using 70% isopropyl alcohol to sterilize it? It seemss that even if you use a new, sterile stopcock, wouldn't it still have possible contamination when you remove the previous stopcock? Thanks. PS) I think you should patent it! I've seen the Myco lid using a metal transferring needle instead of using your silicone tubing, and your lids are superior!

@RealSimpleMushrooms 6 месяцев назад

As long as your sterile tek is good you should have no problem using the stockcock more than once. What i generally like to do is have a small jar with 70% iso in it and I'll just drop the caps in there while I'm filling, then wipe them down and put them back in when I'm done. It's been working great for me.

@user-rt5en6hb1s 4 месяца назад

sorry for the dumb question: Where is the list of items to buy? Can you reply it to me, rather than directing how I find it?

@RealSimpleMushrooms 4 месяца назад

All the links are in the video description

@user-rt5en6hb1s 4 месяца назад

Soooo Sorry, I've had a stroke, and I'm still stroke stoopid. :-( Thank you. I see it, should have looked there and I thank you for shared knowledge. Stroke cause me to experience the near death experience, but also to hallucinate for about 4.5 years. I discovered mrooms in my quest to answer, 'what the hell did I see?' Fortunately, mrooms did not reproduce the quality of graphics that I saw. DMT did not produce the quality of graphics I saw. Nor did acid. Salvia Divinorum came closest, and scared the daylights out of me multiple times. However, SD nearly duplicated the quality of the graphics of my near death experience, but I saw unrealistic devices and mechanisms. There was none of that in what I identify as penducular hallusinosis. I did lay prostate while a small man put his hand inside of my head. He also pulled the nerves in my head such that my eye was outturned after the hallucination 😲. if you want more of these kinds of narratives but with far more detail including being kidnapped and charged with very strange crimes, you might enjoy, I mean appreciate 'The Mysterious Red Light Camera Murder' I'm looking for a publisher, and prissy literary agents are objecting to the use of curse words and low-life expressions such as 'Cops were trying to inventory my dingle berries.' By far, mrooms were the most prosocial of all the things I mentioned. @@RealSimpleMushrooms