DNA replication - 3D 

Learning about replication without actually seeing it makes actually no sense. A must watch for anyone studying biology-related sciences.

Anyone else here because their professor cannot explain things simply?

Almost 8 years ago and this is one of the best DNA replication videos I've ever seen so far! Great visualization!

2024 attendence here ❤

As a senior bio major I shed a tear watching this... so beautiful

Here is a basic & simplified version of the video: BASICS: Adenine --> Thymine Cytosine --> Guanine PREPARING TO REPLICATE: -Helicase splits the DNA for replication -Primase adds RNA bases (primer) to the leading strand. -Why does DNA polymerase require a primer before it adds DNA nucleotides? It is because the RNA primer will have a free -OH group at the end that it can nucleotides to! -DNA Polymerase binds to the primer to start replication! REPLICATION: -The leading strand TEMPLATE will have a daughter strand that is created 5' to 3', in the direction of the REPLICATION FORK (where DNA becomes uncoiled). -The lagging strand TEMPLATE will have a daughter strand that is ALSO created in the 5' to 3' direction, but it will replicate in the OPPOSITE direction of the REPLICATION FORK. -That's not okay! Because it is in the opposite direction of the replication fork, it needs to be made in fragments (Okazaki Fragments). FINISHING TOUCHES: -Exonuclease removes the RNA primers that were added to the start. We don't want RNA in our DNA! -DNA polymerase then adds DNA bases to the missing spots where the RNA primers were. -Ligase makes sure all the fragments are sealed CLOSING: -DNA is described as semi-conservative because there is one old and one new strand of DNA in each DNA. Thanks and hopefully this will help you for your bio test I know it did for me :)

حيا الله طلاب السادس العلمي بويه Final year high school students in Iraq welcome you 🥲🇮🇶

Step 1: Helicase Function: Helicase is the initial enzyme in DNA replication. Its primary function is to unwind the DNA double helix. It accomplishes this by breaking the hydrogen bonds between the complementary base pairs, such as A-T and G-C. By doing so, helicase separates the two DNA strands, creating what is called a "replication fork." This single-stranded DNA region is where the actual replication process will take place. Step 2: Primase Function: Primase follows helicase. Its role is to synthesize RNA primers. Primase adds short RNA sequences, known as primers, to the DNA template strands. These primers are essential because DNA Polymerase, the enzyme responsible for adding new DNA nucleotides during replication, can only extend an existing strand. On the leading strand, primase synthesizes a single RNA primer at the 5' end, providing a starting point for DNA Polymerase. Step 3: DNA Polymerase III Function: DNA Polymerase III is the primary enzyme responsible for DNA synthesis during replication. It adds nucleotides to the growing DNA strand. On the leading strand, DNA Polymerase III synthesizesthe new DNA strand in a continuous manner by extending from the 5' to 3' direction, using the parental DNA strand as a template. This strand doesn't encounter the same challenges as the lagging strand, which requires a more intricate process. Step 4: Exonuclease Function: Exonucleases come into play after DNA Polymerase III. Their function is to remove RNA primers from the DNA template. Once the RNA primers are synthesized by primase, exonucleases degrade them, creating gaps in the DNA strands. On the lagging strand, multiple RNA primers are synthesized at different points along the template to provide starting points for DNA Polymerase. Step 5: DNA Polymerase I Function: DNA Polymerase I is an enzyme responsible for gap-filling. After the removal of RNA primers by exonucleases, DNA Polymerase I replaces the RNA nucleotides with DNA nucleotides. On the lagging strand, DNA Polymerase I works in a discontinuous manner, filling in the gaps created by the removal of RNA primers and synthesizing short DNA fragments called Okazaki fragments. Step 6: Ligase Function: Ligase is the final enzyme involved in DNA replication. Its function is to seal the nicks or gaps in the DNA backbone. It does so by catalyzing the formation of phosphodiester bonds between adjacent nucleotides. On the lagging strand, ligase joins the Okazaki fragments together, ensuring the integrity and continuity of the newly synthesized DNA strand. In conclusion, DNA replication involves two strands: the leading strand, which is synthesized continuously, and the lagging strand, which is synthesized discontinuously in Okazaki fragments. Different enzymes work together to ensure the accurate replication of both strands, preserving genetic information during cell division and ensuring the inheritance of genetic traits.

Take notes, biology teachers. This is how you teach this.

Τhis was actually the only video that made me finally understand it..good job :)

Any neet student here😅

I'd lost all the hope that I'd ever understand replication, after watching this precise , short and easy-understanding video , I'm feeling refreshing now. Hats off to that person who made this...

My biology teacher explained really good and now seeing this video makes dna replication totally clear to me. Thank you!

I’m French and I’ve understood it better in English because professors or unable to EXPLAIN IT CLEARLY IN A SIMPLE WAY !

im literally crying because this is so good. merely reading the process just screwed me. thank you so much for the efforts.

That's a gem. Three & half a minute is more precious than three & half an hour without seeing the animation.

A 3.30 min video made me understand what 3 lectures of 2 hrs each couldn't !

i just passed my biology daily test today about dna after i watched this yesterday night. I wanna give you a BIG thankyou for helping me study☺

this just brings me back to life and makes me forget every "imperfection" I think I have

I learnt DNA replication from ritu man seriously telling mam ki lecture ki baad animation ki jarurat nhi hote h pr m ne yeh dekha or bhi clear ho gaya❤️🔥why not school teacher teach like this ??? Fir bol te h bache nhi pdh te

This video is amazingly incredible. It is precise, up to the point and explains everything in 3:28 min with clear voice with synchronized animation. This video is the best that explains Dna replication on RU-vid. It probably deserves a million likes

Only video that's straight to the point and explicitly states all the steps .. THANK YOU!

I love how the first strand replication process is very simple, while the second strand replication is 100x more complicated lol.

This video is super good in helping visualize the entire replication process. Although I think there was slight oversight in the direction of the DNA strand because in the beginning of the vid, the top strand was labeled 5' on the left side and 3' on the right side, but when DNA polymerase was introduced, the top strand was suddenly 3' on the left side and 5' on the right side.

Life is so perfect.

The best replication animation i have seen yet, thanks a lot!

I'm so glad I speak English. Here in Brazil we have amazing teachers, but i'm a more visual student, so it's a lot easier to learn with 3d videos

This 3 minutes video was more helpful than my madams 3 hours long class. Failed to understood a single word there Thanks!

Finally can understand how lagging strand is replicated. Thank you!

How on earth did someone discover this and all the details?

Amazing❣!!! You have nailed it in minutes. I love how you explained everything about DNA replication. It was perfectly matched with an animation that adds to the total presentation. Your video is fun to watch and easy to understand👏👏

I am an Egyptian student at my third secondary school year and this video helped me to imagine and understand a topic in my curriculum. Thanks a lot!

I don't understand why teachers can't explain like this ; slow and straight to the point . All they do is draw a diagram ( totally not realistic ) and ask us to write notes . And we are like lol what just happened .

It's so interesting to know how our body is made so intricately and it functions in a way that is beyond anything that we would make artificially.

this is the best explanation I've ever watched!!

Very easy to understand whole process of DNA replication through this video...thanks a lot😊

my mind is blown this is the 100th video ive watched tonight and it is the only one i understood god bless

This was by far the best video I've seen of this. Straight and to the point, no annoying music, and simple visuals. Absolutely fantastic!

Very helpful animation! Thanks for visually showing the difference between an RNA primer and an Okazaki fragment; that made it easier to understand.

The best guidance for DNA replication and anyone can understand smoothly...Thank you

I think that my biology teacher didn’t actually know how to explain this so he just resorted to this video lol

Easily the best replication video I've seen to date, thank you. I don't understand why it's so difficult for professors to explain this in a simple step by step process. I get that it's a complex topic, but damn, just go step by step. Stop talking about random enzymes in no discernible order.

しっかり海外でも岡崎フラグメントになってるのなんか感動

this video made me understand the dna replication in a perfect way, the animation is amazing and made it easier to understand especially in the part where the replication is in the opposit direction it was hard to get it at first but after this video i found it actually so simple. thank you for this amazing explication, well done.

As a science teacher this is by far the best replication video out there. Thanks for the clear description!

As a 3D artist, I feel it’s awesome to see these types of animations being used in education. Keep up the great work!

Fantastic explanation...every time before my exams I use to watch this video for better clarity...

My professor litearlly took 8 lessons to explain this process and almost nobody got it, it’s way simpler than I thought thank you

After 20 minutes of watching videos and re-watching them, this one did the most at helping me understand the process.

한국어 자막 달아주신분 감사합니다 덕분에 잘 공부하고 갑니다

This is the best explanation I could've ever got for DNA Replication🔥🔥🔥🔥

This video is so wonderfully helpful. I was struggling thru diagrams in the text trying to get all the weird terms to stick and this is exactly what I needed to see and how I needed it explained. Thants!

Best video ever to show DNA replication! No other video that I could find really explains in a short but well detailed manner. Other videos I feel are for those who are already familiar with the process. This is my first year learning this process and I'm grateful I found this video!!

What took Biology 12 between 3 - 4 weeks to explain, explained in 3 minutes. All I’ve got to say

One of the best videos on the internet, so underrated

Absolutely a great video 😍 was unable to understand replication process but this animated video has put away my all confusion 👍

The old but gold DNA replication 😁 This is so inspirational. Thank you so much, It helps a lot in learning, but also revising.

This was very helpful, I've been studying this for a few days now but I've always had a fuzzy picture of how the lagging strand is formed. Your video cleared everything up. Thank you!

Best and easy way to understand and better than protracted videos

My god this 3 minute video single handedly gets me though the exam. Thank you so so much for ever making this video.

Excellent animation. I wished it showed how topoisomerase did it's work in separating the overcoiled knots of daughter DNA strands.

Out of all the DNA replication videos this one is so good, very detailed and easy to understand thank you

Thank you so much, I had to learn for my biology exam today and this video made things al lot easier to understand!!

one of the best videos on RU-vid NO CAP

This is sooooo amazing with 3d visualisation I have chills when watching this... Gonna watch 9-10 times probably to remember it better...

I must say, for the very first time I can really understand what DNA replication is all about.... Thanks a lot for such a amazing video

This video single handily helped me finally understand the process.

ty alot guys your explanations are as clear as crystal ! neat !

Wow you are a life safer. Thank you so much. This animation should be shown in every biology class on this topic. Edit: I suggested it to my teacher. It might have an impact. Good job!!!

Such a short clip is worth more than a thousand books.

Perfectly summarises everything.

The best genetics video I've watched so far

Short and concise! Best explanation I found so far on the internet. It also shows how easy it is for this process to make small mistakes hereby distorting the DNA replication process with all the consequences that follow.

정말정말정말 감사합니다 Thank you sooo much 자막 달아주신분두요ㅜㅜ

Wow, what an amazing video! Incredible animation of this complicated process...helped me a lot with my schoolwork.

That's the video I was looking for...excellent animation

can't believe i haven't found this earlier after so much of my time wasted trying to look for a simple explanation like this. thank you so much i finally understand it.

My life I must be a visual learner this is the first time i have ever had a clue about the lagging strand

3 minutes and you made everything clear luv uuuuu

Simple explanation. Beautiful animation. I couldn't possibly wrap my mind around this Okazaki business before I watched this.

this is so magical that i can’t stop rewatching

Biology teachers should learn how to teach replication and some other topics soo easily as in this video. Finally I understood Replication with the help of this video. Thanks a lot 👍👍👏👏😊😊

Best video ive seen on DNA replication!

Simple and comprehensive video .. At first I thought it was an obscure concept , but after watching this video I got it...

This is one of the best videos for explaining DNA replication, thank you!

One of the most helpful video I've ever seen. Thanks for it.

this was way better than what i understood in class so thank you!

Isn't this enough to give us a thought about why r we created so perfectly ?

hands down the best video explaining this concept, and trust me I looked at lots

Extremely helpful! You have done a wonderful job. Thanks a lot.

Learned more from the 3 minute video than I did from my professors 2 hour long lecture that mainly consisted of word vomit and pointing at words on a PowerPoint. Thanks

I said it and repeat : It's the best type of explanation ever.

Finally I'll be visualize the process of DNA replication

Best animation ever,and the lady was explaining very good👏👏👏👏👏👏👏👏👏👏

映像で見るとやはり分かりやすい。復習する際にも、動画で流れを確認すると思い出しやすい。

Beautiful. I am in love with these videos. So easy to understand for a concept I have been struggling to understand for a long time. A big thank you to the one who took pains to make this video. Please keep providing students with such useful concepts in easy form.

So much easier to understand when its visualized

Thank you very much! very simple explanation. I used this video for my genetics exam and it was very useful

so simplified and yet all points covered I will cry buckets right now .

A very easy and clear way of understanding this, thankyou