Please do make more of the DNA videos! I used your videos last year when revising for AS and it was so helpful, it's easier to understand when someone explains it instead of just reading a book all the time. Thankyou :)
This video is soo useful! going to recommend it to many people who are also struggling with this topic! am i the only person who feels like the sound of the video is really low though?
I am doing an intensive A-level in biology and your videos are absolutely fantastic for all topics. Thank you very much! You have gained a subscriber :D I have also been letting my classmates for AS and A2 know about you. ;
+Mr Pollock I know you must be so busy but I really do hope you make some more on Unit 5 genetics! I really struggle with that section and you do such a good job at explaining biology. Thank you for all your other videos :)
For the Fluorescent Markers - are you saying that we extract the GFP gene from the Jelly fish (using restriction endonuclease I suppose?) and then insert it in into the plasmid, and then insert the gene we are interested in onto the GFP gene we just inserted into the plasmid (using restriction endonuclease again)? I'm so confused!
For the replica plating method, would it be possible to do it the other way around, so the DNA fragment is inserted into the segment of DNA in the plasmid containing the Ampicillin gene instead of inserting it into the segment with the Tetracycline gene? So when the plasmid is inserted back into the bacteria successfully, it wouldn't be resistant to Ampicillin but would only be resistant to Tetracycline. If this were the case would we first grow the bacteria cells in the medium containing Tetracycline and the copy it onto a replica plate containing Ampicillin? And therefore the successful bacteria would be the ones killed by the A plate but survived on the T plate? I'm just wondering if this method would be possible because I'm using 3 different text books and they all use the example where the DNA fragment is only inserted into the tetracycline segment of plasmid at the start... Also what if the plasmid only contains one gene for antibiotic resistance (So could only be resistant to say, Ampicillin), could the replica plating method still be used in any way? And if it can't, why not?
Extremely confused.. Why would you cut up a gene forming sticky ends just to put them back together using the DNA ligase? How does the DNA ligase form a double stranded sugar phosphate backbone?
wait can someone explain to me how this actually clones the DNA? it seems to me like it just allows the protein to be made but it doesnt actually make more of the same dna unlike pcr, just utilises it. or do people harvest the rna the cell produces? does the bacteria replicate the plasmid once within? im lost :( (great video though!)
