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Enzyme Assay, Enzyme Activity and Specific Activity 

Andrey K
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30 янв 2015

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Комментарии : 54   
@itogisch
@itogisch 9 лет назад
thanks for the clear explanation about enzyme assays. i've been trying to find an explanation without having to read 50 pages about all types of assays.
@AKLECTURES
@AKLECTURES 9 лет назад
itogisch lol :-) you're welcome
@DrNem0
@DrNem0 8 лет назад
Just wanted to say thank you for making this video, and explaining concepts the way you do. :) I've just started my degree in biochem, kind of been struggling in my first term because my mind is a mess! The way this video is structured however just made me focus in a way I haven't been able to for a while. I think it's the fact you started out with the real-life applications of the thing being studied, which is often not done in textbooks in the very beginning making it difficult (for me at least) to engage in the theory. The notes work well, it acts as a visual aid to where I am in the theory and I appreciate how you repeat each key point a final time - it's as if you know my mind would wander off and then by the time I become aware of this and alert you finalise the point I should've been paying attention to, but you don't overdo it as though to appear patronising. Overall, a very balanced video (in terms of different methods of learning), really taught me some things on the way I best learn too. Keep doing what you're doing!
@about_shreya
@about_shreya 3 года назад
Same here.. Just started with the course n the videos helped a lot
@APOLOnl
@APOLOnl 8 лет назад
this is great, thanks alot 👌 a much better explenation than any of my schoolteachers. They use to over complicate things. This is a verry clear and well spoken lecture
@ozzDeveveloperOpenForWork
@ozzDeveveloperOpenForWork 7 лет назад
great explanation! I'm taking biocehm and your videos are helping a lot.
@oluwapeluminexus
@oluwapeluminexus Год назад
You are such a blessing. I was excited to see that you have a video on this topic i needed clarity about because I know you would do absolute justice to it and you didn't disappoint. Thanks for all you put into this. God bless you.
@fatiibnmajdoub7484
@fatiibnmajdoub7484 8 лет назад
Thank you so much for the clarification. Really appreciate your efforts. Best regards
@prudencetchouakeubetnga5718
@prudencetchouakeubetnga5718 6 лет назад
thank you very much for your videos. you have help me a lot in genetic, enzymology, biochemistry and physic domain to pass my exams. Thanks a lot l really enjoy your videos
@sfavrgra
@sfavrgra 2 года назад
I honestly couldn't understand my lecterurs notes so by watching this video it does really helpful. Thank you so much!
@harshilsuthar5350
@harshilsuthar5350 8 лет назад
thank you very much.....i am confuse between enzyme activity and specific activity after showing this all doubts goes into dustbin...good
@ahc3363
@ahc3363 4 года назад
7:05 screen shot
@animeandstuff5377
@animeandstuff5377 3 года назад
lol nice of you but i seen ur comment after having to screenshot twice real quick lol
@dr.danishraja497
@dr.danishraja497 3 года назад
Thank u brother 😂 🥰
@shilpasaroj730
@shilpasaroj730 4 года назад
Thank you so much for explaining this in simplified way 👏I was trying to understand this topic from books, google but ended up getting more confused.
@loisvankampen3256
@loisvankampen3256 8 лет назад
Thank you! I think I will be watching your video's for the entire weekend before my biochemistry exam :-)
@kibanaru221
@kibanaru221 3 года назад
That literally me rn 😂 4 years later and this hold true
@loisvankampen3256
@loisvankampen3256 3 года назад
@@kibanaru221 I passed so good luck!
@applejackzo
@applejackzo 7 лет назад
You're amazing.
@Petinency
@Petinency 6 лет назад
Easiest lectures, thank you!!
@caterine4604
@caterine4604 5 лет назад
YOU ARE MY FAV PERSON IN THE WORLD
@Ozchuck
@Ozchuck 7 лет назад
this helped. thank you.
@jziebert1
@jziebert1 4 года назад
Excellent, well done!
@cybersufer_27
@cybersufer_27 Год назад
You're an excellent teacher! Thank you so much! 🤓
@TomasBruno-ww6tg
@TomasBruno-ww6tg 4 года назад
Hi. This video is great! I only have one commentary about it: the enzyme activity can actually be defined in many other ways, since it can only be measured indirectly, the units and the definition of the activity scale depends on the experiment that takes place in order to obtain such value. I think it's important to reinforce this to prevent anyone to believing this is the only possibility.
@saddamali666
@saddamali666 5 лет назад
You're the best I wish you did a CARS series!
@1730298
@1730298 8 лет назад
Thanks for your best video , its really helpful just to say if you could make one more video about how to calculate other related things such as Total protein, Total activity, Yield, Specific activity and purification factor . thanks again
@duvetumelo7265
@duvetumelo7265 3 года назад
Quite a super tutor👍
@ankushsaini9977
@ankushsaini9977 4 года назад
Thanks 😊😘 for uploading video on this topic
@alyssa-abcde
@alyssa-abcde 4 года назад
Thank you very much, sir. You've helped me a lot in many, many ways.
@sakthivelb9074
@sakthivelb9074 Год назад
what is the sample used for enzyme assay
@xuejingli1657
@xuejingli1657 3 года назад
excellent, thank you very much!
@Abbyblackblack
@Abbyblackblack 6 лет назад
thank you so much !
@arunkumars6257
@arunkumars6257 Год назад
Thank you so much!
@ashareelsrrr8089
@ashareelsrrr8089 Год назад
Helpful video
@lorettadass3335
@lorettadass3335 3 года назад
Well explained
@plutoniumiscool
@plutoniumiscool 4 года назад
What assay would you recomend for protease activity with microgram concentration?
@christinac1195
@christinac1195 6 лет назад
thank youuu
@naeemamatuoog9539
@naeemamatuoog9539 8 лет назад
I would like to thank you for the clarification. can you help me . plz ? I want to know How do I test the lipase enzyme activity in the lab? (Laboratory experience for their immobilization lipase process
@bestcameroon9051
@bestcameroon9051 5 лет назад
Hello Everyone, please I have a question regarding the calculation of relative activity of enzyme during regular intervals of Time over a long time period ( 3 weeks for example). I am working on the immobilization of cellulase enzyme for cellulose hydrolysis and I would like to estimate thé relative activity of immobilized cellulase over 1, 2 and 3 weeks. As the activity of cellulase is defined as the amount of glucose produced per minutes, its activity over a long Time ( 1, 2 or 3 weeks) will be significantly affected by the incubation Time. Then I would like to ask how Can i calculate this rekative activity taking in consideration the reaction Time ( in minutes).
@nananor3656
@nananor3656 5 лет назад
What does the enzyme that has high absorabnce with lower rate of change mean?
@LovelyLife365
@LovelyLife365 2 года назад
Excellently described Sir
@gunslingerxxi6194
@gunslingerxxi6194 7 лет назад
How to calculate the enzyme concentration?
@bhawanagahlot9540
@bhawanagahlot9540 4 года назад
I loved every vedio... But this vedio was just above my mind... I was unable to understand it
@ooiTzAmii
@ooiTzAmii 7 лет назад
How does one calculate percentage recovery?
@josedilsondasilva8231
@josedilsondasilva8231 4 года назад
Hello. How to turn activity 1: 10000 to U / mg? Thank you very much.
@eruishikawa9978
@eruishikawa9978 4 года назад
How do you find out the moles of product? Lets say we have various conecentrations of an enzyme and want to figure out the no. of moles of each sample. How would you do that?
@tanvi_1840
@tanvi_1840 2 года назад
My question exactly.
@arianalevitt3686
@arianalevitt3686 2 года назад
not all heros where capesthanks dude:)
@josefinesfilmtube
@josefinesfilmtube 2 года назад
why can you not use specific activity when high purity is required
@sakthivelbaskar1586
@sakthivelbaskar1586 Год назад
How to calculate the concentration
@adrinashafiqah1759
@adrinashafiqah1759 3 года назад
Survived online classes
@himanibhatt1170
@himanibhatt1170 2 года назад
Is there anything this channel doesn't offers ???
@nandini1149
@nandini1149 Год назад
Write the words in big, because I can't understanding,it's so small wards
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